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Structural characterization of the lipid A from the LPS of the haloalkaliphilic bacterium Halomonas pantelleriensis

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Abstract

Halomonas pantelleriensis DSM9661Τ is a Gram-negative haloalkaliphilic bacterium isolated from the sand of the volcanic Venus mirror lake, closed to seashore in the Pantelleria Island in the south of Italy. It is able to optimally grow in media containing 3–15 % (w/v) total salt and at pH between 9 and 10. To survive in these harsh conditions, the bacterium has developed several strategies that probably concern the bacteria outer membrane, a barrier regulating the exchange with the environment. In such a context, the lipopolysaccharides (LPSs), which are among the major constituent of the Gram-negative outer membrane, are thought to contribute to the restrictive membrane permeability properties. The structure of the lipid A family derived from the LPS of Halomonas pantelleriensis DSM 9661T is reported herein. The lipid A was obtained from the purified LPS by mild acid hydrolysis. The lipid A, which contains different numbers of fatty acids residues, and its partially deacylated derivatives were completely characterized by means of ESI FT-ICR mass spectrometry and chemical analysis. Preliminary immunological assays were performed, and a comparison with the lipid A structure of the phylogenetic proximal Halomonas magadiensis is also reported.

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Abbreviations

ESI FT-ICR:

Electrospray ionization Fourier transform-ion cyclotron resonance

GC–MS:

Gas chromatography–mass spectrometry

EI–MS:

Electron ionization–mass spectrometry

NMR:

Nuclear magnetic resonance

CID MS/MS:

Collision-induced dissociation

Th1:

T helper 1

Th2:

T helper 2

IFN-γ:

Interferon-γ

TNF-α:

Tumor necrosis factor-α

IL-4:

Interleukin 4

IL-10:

Interleukin 10

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Authors and Affiliations

  1. National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, County Dublin, Ireland

    Sara Carillo

  2. Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy

    Giuseppina Pieretti, Angela Casillo, Rosa Lanzetta & Maria Michela Corsaro

  3. Division of Bioanalytical Chemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 10, 23845, Borstel, Germany

    Buko Lindner

  4. Istituto di Chimica Biomolecolare ICB-CNR, Via Campi Flegrei 34, 80078, Pozzuoli, Italy

    Ida Romano & Barbara Nicolaus

  5. Department of Biology, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy

    Michelangelo Parrilli

  6. Department of Experimental Medicine, Second University of Naples, via de Crecchio 7, 80138, Naples, Italy

    Mariateresa Giuliano & Marcella Cammarota

Authors
  1. Sara Carillo
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  2. Giuseppina Pieretti
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  3. Angela Casillo
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  4. Buko Lindner
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  5. Ida Romano
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  6. Barbara Nicolaus
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  7. Michelangelo Parrilli
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  8. Mariateresa Giuliano
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  9. Marcella Cammarota
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  10. Rosa Lanzetta
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  11. Maria Michela Corsaro
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Corresponding author

Correspondence to Maria Michela Corsaro.

Additional information

Communicated by A. Oren.

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Carillo, S., Pieretti, G., Casillo, A. et al. Structural characterization of the lipid A from the LPS of the haloalkaliphilic bacterium Halomonas pantelleriensis . Extremophiles 20, 687–694 (2016). https://doi.org/10.1007/s00792-016-0858-2

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  • DOI: https://doi.org/10.1007/s00792-016-0858-2

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