Skip to main content
SpringerLink
Log in

Salinicoccus salitudinis sp. nov., a new moderately halophilic bacterium isolated from a saline soil sample

  • Original Paper
  • Published:
Extremophiles Aims and scope Submit manuscript

Abstract

A novel pale-yellow-pigmented, moderately halophilic, facultatively alkaliphilic, non-motile, non-spore-forming, catalase- and oxidase-positive, obligately aerobic Gram-positive coccus, strain YIM-C678T was isolated from a saline soil sample collected from a hypersaline habitat in the Qaidam basin, northwest China. The organism grew at 4–37°C and pH 6.0–11.0, with optimum growth at 25°C and pH 8.0. Strain YIM-C678T grew optimally in the presence of 10–12% (w/v) NaCl and growth was observed in 1–25% (w/v) NaCl. The cell wall murein type was l-Lys-Gly5. Major cellular fatty acids were anteiso-C15:0, iso-C15:0, iso-C16:0 and C16:0. Menaquinone 6 (MK-6) was the major respiratory quinone. The DNA G + C content was 46.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain YIM-C678T belonged to the family Staphylococcaceae and was most closely related to the eight described species of the genus Salinicoccus with sequence similarities from 92.2 (S. luteus YIM 70202T) to 97.5% (S. kunmingensis YIM Y15T). The DNA–DNA relatedness between strain YIM-C678T and S. kunmingensis YIM Y15T was 35.4%. Chemotaxonomic data and 16S rRNA gene sequence analysis supported the affiliation of strain YIM-C678T with the genus Salinicoccus. The combination of phylogenetic analysis, phenotypic characteristics, chemotaxonomic differences and DNA–DNA hybridization data supported the view that the bacterium represents a novel species of the genus Salinicoccus, for which the name Salinicoccus salitudinis sp. nov. is proposed, with YIM-C678T (=DSM 17846 = CGMCC 1.6299) as the type strain.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Aslam Z, Lim JH, Im WT, Yasir M, Chung YR, Lee ST (2007) Salinicoccus jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 57: 633–638

    Article  PubMed  CAS  Google Scholar 

  • Atlas RM (1993) Handbook of microbiological media. Parks LC (Ed.) CRC, Boca Raton

    Google Scholar 

  • Chen HH, Li WJ, Tang SK, Kroppenstedt RM, Stackbrandt E, Xu LH, Jiang CL (2004) Corynebacterium halotolerans sp. nov., isolated from saline soil in the west of China. Int J Syst Evol Microbiol 54:779–782

    Article  PubMed  CAS  Google Scholar 

  • Chen YG, Cui XL, Pukall R, Li HM, Yang YL, Xu LH, Wen ML, Peng Q, Jiang CL (2007) Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, southwest China. Int J Syst Evol Microbiol 57 (in press)

  • Cui XL, Mao PH, Zeng M, Li WJ, Zhang LP, Xu LH, Jiang CL (2001) Streptomonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51:357–363

    Google Scholar 

  • Cui XL, Schumann P, Stackebrandt E, Kroppenstedt RM, Pukall R, Xu LH, Rohde M, Jiang CL (2004) Myceligenerans xiligouense gen. nov., sp. nov., a novel hyphae-forming member of the family Promicromonosporaceae. Int J Syst Evol Microbiol 54:1287–1293

    Article  PubMed  CAS  Google Scholar 

  • De Ley J, Cattoir H, Reynaerts A (1970) The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142

    Article  PubMed  Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: An approach using the bootstap. Evolution 39:783–791

    Article  Google Scholar 

  • Felsenstein J (1993) PHYLIP (phylogenetic inference package), version 3.5. Distributed by the author. Department of Genetics, University of Washington, Seattle, USA

  • Franca L, Rainey FA, Nobre MF, Costa MS (2006) Salinicoccus salsiraiae sp. nov.: a new moderately halophilic gram-positive bacterium isolated from salted skate. Extremophiles 10:531–536

    Article  PubMed  CAS  Google Scholar 

  • Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Krieg NR, Philips GB (1981) Manual of methods for general bacteriology. Am Soc Microbiol, Washington, DC

    Google Scholar 

  • Gregersen T (1978) Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:123–127

    Article  Google Scholar 

  • Hiraishi A, Ueda Y, Ishihara J, Mori T (1996) Comparative lipoquinone analysis of influent sewage and activated sludge by highperformance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469

    Article  CAS  Google Scholar 

  • Hopwood DA, Bibb MJ, Chater KF, Kieser T, Bruton CJ, Kieser HM, Lydiate DJ, Smith CP, Ward JM (1985) Preparation of Chromosomal, plasmid and phage DNA. In Genetic manipulation of Streptomyces: a laboratory manual, F. Crowe and Sons, Norwich, pp 79–80

    Google Scholar 

  • Huß VAR, Festl H, Schleifer KH (1983) Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192

    Google Scholar 

  • Jahnke KD (1992) BASIC computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15:61–73

    Article  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  PubMed  CAS  Google Scholar 

  • Kluge AG, Farris FS (1969) Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32

    Article  Google Scholar 

  • Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245

    Article  PubMed  CAS  Google Scholar 

  • Kushner DJ (1993) Growth and nutrition of halophilic bacteria. In: Vreeland RH, Hochstein LI (eds) The biology of halophilic bacteria. CRC, Boca Raton, pp 87–103

    Google Scholar 

  • Li WJ, Zhang YQ, Schumann P, Tian XP, Zhang YQ, Xu LH, Jiang CL (2006) Sinococcus qinghaiensis gen. nov., sp. nov., a novel member of the order Bacillales from a saline soil in China. Int J Syst Evol Microbiol 56:1189–1192

    Article  PubMed  CAS  Google Scholar 

  • Li WJ, Schumann P, Zhang YQ, Xu P, Chen GZ, Xu LH, Stackebrandt E, Jiang CL (2005) Proposal of Yaniaceae fam. nov. and Yania flava sp. nov. and emended description of the genus Yania. Int J Syst Evol Microbiol 55:1933–1938

    Article  PubMed  CAS  Google Scholar 

  • Mandel M, Marmur J (1968) Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206

    Article  Google Scholar 

  • Marquez MC, Ventosa A, Ruiz-Berraquero F (1990) Marinococcus hispanicus, a new species of moderately halophilic Gram-positive cocci. Int J Syst Bacteriol 40:165–169

    Google Scholar 

  • Pakdeeto A, Tanasupawat S, Thawai C, Moonmangmee S, Kudo T, Itoh T (2007) Salinicoccus siamensis sp. nov., isolated from fermented shrimp paste in Thailand. Int J Syst Evol Microbiol 57:2004–2008

    Article  PubMed  CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4(4):406–425

    PubMed  CAS  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16

    Google Scholar 

  • Schleifer KH (1985) Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156

    CAS  Google Scholar 

  • Schumann P, Cui XL, Stackebrandt E, Kroppenstedt RM, Xu LH, Jiang CL (2004) Jonesia quinghaiensis sp. nov., a new member of the suborder Micrococcineae. Int J Syst Evol Microbiol 54:2181–2184

    Article  PubMed  CAS  Google Scholar 

  • Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Google Scholar 

  • Staneck JL, Roberts GD (1974) Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231

    PubMed  CAS  Google Scholar 

  • Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849

    CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  PubMed  CAS  Google Scholar 

  • Ventosa A, Quesada E, Rodriguez-Valera F, Ruiz-Berraquero F, Ramos-Cormenzana A, Kocur M (1982) Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128:1959–1968

    Google Scholar 

  • Ventosa A, Marquez MC, Ruiz-Berraquero F, Kocur M (1990) Salinicoccus roseus gen. nov., sp. nov., a new moderately halophilic Gram-positive coccus. Syst Appl Microbiol 13:29–33

    Google Scholar 

  • Ventosa A, Marquez MC, Weiss N, Tindall BJ (1992) Transfer of Marinococcus hispanicus to the genus Salinicoccus as Salinicoccus hispanicus comb. nov. Syst Appl Microbiol 15:530–534

    Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, other authors (1987) International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  • Zhang YQ, Yu LY, Liu HY, Zhang YQ, Xu LH, Li WJ (2007) Salinicoccus luteus sp. nov., isolated from a desert soil. Int J Syst Evol Microbiol 57:1901–1905

    Article  PubMed  Google Scholar 

  • Zhang YQ, Schumann P, Yu LY, Liu HY, Zhang YQ, Xu LH, Stackebrandt E, Jiang CL, Li WJ (2006) Zhihengliuella halotolerans gen. nov., sp. nov., a novel member of the family Micrococcaceae. Int J Syst Evol Microbiol 56:1018–1023

    Google Scholar 

  • Zhang YQ, Schumann P, Li WJ, Chen GZ, Tian XP, Stackebrandt E, Xu LH, Jiang CL (2005) Isoptericola halotolerans sp. nov., a novel actinobacterium isolated from saline soil from Qinghai province, northwest China. Int J Syst Evol Microbiol 55:1867–1870

    Article  PubMed  CAS  Google Scholar 

  • Zhang W, Xue Y, Ma Y, Zhou P, Ventosa A, Grant WD (2002) Salinicoccus alkaliphilus sp. nov., a novel alkaliphile and moderate halophile from Baer Soda lake in inner Mongolia autonomous region, China. Int J Syst Evol Microbiol 52:789–793

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (NSFC) (30460004, 30660004, 30760006, 30560001), SRF for ROCS (SEM), the Yunnan Provincial Sciences and Technology Department (2005PY01-1, 2004C0002Z, 2006C0006M) and the National Basic Research Program of China (973 Program, No. 2004CB719601). We are grateful to Prof. Dr. Hans G. Trüper for recommending the proper etymology and to Ms. Li-Xia Duan for her excellent technical assistance.

Author information

Authors and Affiliations

  1. Yunnan Institute of Microbiology, Key Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan, 650091, People’s Republic of China

    Yi-Guang Chen, Xiao-Long Cui, Wen-Jun Li, Li-Hua Xu, Meng-Liang Wen, Qian Peng & Cheng-Lin Jiang

  2. College of Bio-resources and Environmental Science, Jishou University, Jishou, Hunan, 416000, People’s Republic of China

    Yi-Guang Chen

Authors
  1. Yi-Guang Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiao-Long Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wen-Jun Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Li-Hua Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Meng-Liang Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qian Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Cheng-Lin Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xiao-Long Cui.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, YG., Cui, XL., Li, WJ. et al. Salinicoccus salitudinis sp. nov., a new moderately halophilic bacterium isolated from a saline soil sample. Extremophiles 12, 197–203 (2008). https://doi.org/10.1007/s00792-007-0116-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00792-007-0116-8

Keywords

Search

Navigation