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Genome and proteome characterization of the psychrophilic Flavobacterium bacteriophage 11b

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Abstract

Virion DNA of bacteriophage 11b (Φ11b), which infects a psychrophilic Flavobacterium isolate from Arctic sea-ice, was determined to consist of 36,012 bp. With 30.6% its GC content corresponds to that of host-genus species and is the lowest of all phages of Gram-negative bacteria sequenced so far. Similarities of several of 65 predicted ORFs, genome organization and phylogeny suggest an affiliation to ‘mesophilic’ nonmarine siphoviruses, e.g. to bacteriophages SPP1 and HK97. Early genes presumably encode an essential recombination factor (ERF), a single strand binding (SSB) protein, an endonuclease, and a DNA methylase. The late gene segment is likely to contain a terminase, portal, minor head, protease and a major capsid gene. Five ORFs exhibited similarities to Bacteroidetes species and seem to reflect the host specificity of the phage. Among PAGE-separated virion proteins that were identified by MALDI-ToF mass spectrometry are the portal, the major capsid, and a putative conserved tail protein. The Φ11b genome is the first to be described of a cultivated virus infecting a psychrophilic host as well as a Bacteroidetes bacterium.

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Acknowledgments

We thank Hansjörg Lehnherr for critical reading of the manuscript and Dr. Steffen Krüger for helpful comments during the sequencing and sequence assembly procedure. We are grateful for the support of Michael Hecker in the analysis of the phage proteome.

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Authors and Affiliations

  1. Institute of Marine Biotechnology, W.-Rathenau-Str. 49a, 17489, Greifswald, Germany

    Michael Borriss & Thomas Schweder

  2. Max-Planck-Institute for Marine Microbiology, Celsius-Str. 1, 28359, Bremen, Germany

    Thierry Lombardot & Frank Oliver Glöckner

  3. Institute of Microbiology, Ernst-Moritz-Arndt-University, F.-L.-Jahn-Str. 15, 17487, Greifswald, Germany

    Dörte Becher & Dirk Albrecht

  4. Institute of Pharmacy, Ernst-Moritz-Arndt-University, F.-L.-Jahn-Str. 17, 17487, Greifswald, Germany

    Thomas Schweder

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  1. Michael Borriss
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  2. Thierry Lombardot
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  3. Frank Oliver Glöckner
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  4. Dörte Becher
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  6. Thomas Schweder
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Correspondence to Thomas Schweder.

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Communicated by K. Horikoshi

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Borriss, M., Lombardot, T., Glöckner, F.O. et al. Genome and proteome characterization of the psychrophilic Flavobacterium bacteriophage 11b. Extremophiles 11, 95–104 (2007). https://doi.org/10.1007/s00792-006-0014-5

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  • DOI: https://doi.org/10.1007/s00792-006-0014-5

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