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Salivary microbiome diversity in Chinese children with various caries states

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Abstract

Objective

This study aimed to explore oral microbiome diversity among children with various caries status based on dmft scores.

Methods

A total of 320 children aged 3–5 years were recruited, with 66 healthy children and 254 children affected by dental caries. According to dmft scores, these children with dental caries were classified as “mild group” (dmft score 1–3), “moderate group” (dmft score 4–6), and “severe group” (dmft score 7–14). Healthy children with dmft score of 0 served as control group. Illumina MiSeq sequencing was employed to analyze all salivary samples collected from these children.

Results

The salivary microbial diversity among four groups was similar (p > 0.05). A total of five bacterial genera were highly abundant in the control group including Bergeyella, Acidimicrobiales, Acidimicrobiia, Halomonas, and Blautia (p < 0.05). For mild group, there were nine bacterial genera identified to be predominant: Porphyromonadaceae, Porphyromonas, Enterobacteriales, Enterobacteriaceae, Weissella, Leuconostocaceae, Alphaproteobacteria, Stenotrophomonas, and Rhizobiales (p < 0.05). Only one genus, Aggregatibacter was predominant in moderate group (p < 0.05). There were six bacterial genera (Alistipes, Lachnoclostridium, Escherichia-Shigella, Romboutsia, Sphingomonadales, and Denitratisoma) enriched in severe group (p < 0.05).

Conclusion

Oral microbial profile was different in children with various caries status based on dmft scores.

Clinical relevance

The results might be beneficial to deeply understand microbiological diversity of early childhood caries (ECC) at various stages and inform effective strategies for ECC prevention.

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Data availability

Data will be uploaded as supplementary material and deposited in a public accessible database.

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Acknowledgements

We thank all of the subjects recruited for this project.

Funding

This study was financially supported by Innovation Group Project from the Fundamental Research Funds for the Central Universities (Northwest Minzu University; no. 31920180017).

Author information

Authors and Affiliations

  1. Department of Dentistry, Key Laboratory of Oral Diseases of Gansu Province, Key Laboratory of Stomatology of State Ethnic Affairs Commission, Northwest Minzu University, No 1 Xibeixincun Baiyin Road, Chengguan District, Lanzhou, 730030, Gansu Province, China

    Lingli Wu, Bin Ma, Zhongming Ma, Qingtao Meng, Zhiqiang Li & Haijing Zhou

  2. Department of Dentistry, Lanzhou University, Lanzhou, Gansu Province, China

    Fei Yu

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  1. Lingli Wu
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Contributions

Lingli Wu contributed to the design of the work and supervised the data collection, the data analysis, and manuscript preparation. Zhiqiang Li and Haijing Zhou contributed to supervise the data collection, data analysis, and critical revisions. Bin Ma contributed to the data analysis and critical revisions. Fei Yu, Zhongming Ma, and Qingtao Meng contributed to the data collection and manuscript revisions. All of the authors have read and approved the final version of the manuscript submitted for publication.

Corresponding authors

Correspondence to Zhiqiang Li or Haijing Zhou.

Ethics declarations

Ethical approval

This study was approved by the ethics committee of the Medical College of Northwest Minzu University (XBMU-YX-2018020). All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Parental written consent for all children included in this study was obtained.

Conflict of interest

The authors declare no competing interests.

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Wu, L., Ma, B., Yu, F. et al. Salivary microbiome diversity in Chinese children with various caries states. Clin Oral Invest 27, 773–785 (2023). https://doi.org/10.1007/s00784-022-04825-y

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