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Strategy for reducing cytotoxicity and obtaining esthetic efficacy with 15 min of in-office dental bleaching

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Abstract

Objectives

Evaluate in vitro the esthetic efficacy and cytotoxicity of a bleaching gel containing 35% hydrogen peroxide (BG-35%H2O2), applied for different time intervals, on enamel coated or not with polymeric biomaterials.

Materials and methods

Nanofiber scaffolds (NSc) and a primer catalyst (PrCa) were used to coat the bovine enamel/dentin discs before the application of BG-35%H2O2, according to the following groups: G1—negative control (NC, without treatment); G2, G3, and G4—BG-35%H2O2 applied for 3 × 15, 2 × 15, and 15 min; G5, G6, and G7—BG-35%H2O2 applied on enamel coated with NSc and PrCa for 3 × 15; 2 × 15, and 15 min, respectively. The culture medium with components of gel diffused through the discs was applied on MDPC-23 cells, which were evaluated regarding to viability (VB), integrity of the membrane (IM), and oxidative stress (OxS). The quantity of H2O2 diffused and esthetic efficacy (ΔE/ΔWI) of the dental tissues were also analyzed (ANOVA/Tukey; p < 0.05).

Results

Only G7 was similar to G1 regarding VB (p > 0.05). The lowest value of H2O2 diffusion occurred in G4 and G7, where the cells exhibited the lowest OxS than G2 (p < 0.05). Despite G5 showing the greatest ΔE regarding other groups (p < 0.05), the esthetic efficacy observed in G7 was similar to G2 (p > 0.05). ΔWI indicated a greater bleaching effect for groups G5, G6, and G7 (p < 0.05).

Conclusion

Coating the dental enamel with polymeric biomaterials reduced the time and the cytotoxicity of BG-35%H2O2.

Clinical significance.

Coating the dental enamel with polymeric biomaterials allows safer and faster BG-35%H2O2 application.

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Funding

The work was supported by financial support from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (grant 88887.513642/2020–00), the Fundação de Amparo à Pesquisa do Estado de São Paulo (grant 2017/22739–9) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (grants 408721/2018–9 and 302047/2019–0).

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Authors and Affiliations

  1. Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, São Paulo State University (Unesp), Rua Humaitá, 1680, Araraquara, SP, 14801-903, Brazil

    Uxua Ortecho-Zuta, Carla Caroline de Oliveira Duque, Rafael Antonio de Oliveira Ribeiro & Maria Luísa Leite

  2. Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Rua Alameda Dr. Octávio Pinheiro Brisolla, 9-75, Bauru, SP, 17012-901, Brazil

    Diana Gabriela Soares

  3. Departament of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, University São Paulo State (Unesp), Rua Humaitá, 1680, Araraquara, SP, 14801-903, Brazil

    Josimeri Hebling

  4. Department of Restorative Dentistry, Araçatuba, School of Dentistry University São Paulo State University (Unesp), Rua José Bonifácio 1193, Vila Mendonça, 16015-050, Brazil

    André Luiz Fraga Briso

  5. Department of Physiology and Pathology, Araraquara School of Dentistry, São Paulo State University (Unesp), Rua Humaitá, Araraquara, SP, 168014801-903, Brazil

    Carlos Alberto de Souza Costa

Authors
  1. Uxua Ortecho-Zuta
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  2. Carla Caroline de Oliveira Duque
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  3. Rafael Antonio de Oliveira Ribeiro
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  4. Maria Luísa Leite
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  5. Diana Gabriela Soares
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  6. Josimeri Hebling
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  7. André Luiz Fraga Briso
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  8. Carlos Alberto de Souza Costa
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Correspondence to Carlos Alberto de Souza Costa.

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Ortecho-Zuta, U., de Oliveira Duque, C.C., de Oliveira Ribeiro, R.A. et al. Strategy for reducing cytotoxicity and obtaining esthetic efficacy with 15 min of in-office dental bleaching. Clin Oral Invest 26, 4099–4108 (2022). https://doi.org/10.1007/s00784-022-04379-z

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