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Odontogenesis-related candidate genes involved in variations of permanent teeth size

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Abstract

Objective

The aim of the study was to evaluate the association between genetic polymorphisms in RUNX2, BMP4, BMP2, TGFβ1, EGF, and SMAD6 and variations in permanent tooth size (TS).

Materials and methods

This cross-sectional study evaluated 110 individuals’ dental casts to determine the maximum tooth crown size of all fully erupted permanent teeth (third molars were excluded) in the mesiodistal (MD) and buccolingual (BL) dimensions. Genomic DNA was obtained from the epithelial cells of the oral mucosa to evaluate the genetic polymorphisms in RUNX2 (rs59983488 and rs1200425), BMP4 (rs17563), BMP2 (rs235768 and rs1005464), TGFβ1 (rs1800470), EGF (rs4444903), and SMAD6 (rs2119261 and rs3934908) through real-time PCR. The data were submitted to statistical analysis with a significance level of 0.05.

Results

The genetic polymorphisms rs59983488, rs1200425, rs17563, rs235768, rs1005464, rs1800470, and rs4444903 were associated with MD and BL TS of the upper and lower arches (p < 0.05). The polymorphism rs2119261 was associated with variation in TS only in the upper arch (p < 0.05). The rs3934908 was not associated with any TS measurement (p > 0.05).

Conclusions

In summary, this study reports novel associations between variation in permanent TS and genetic polymorphisms in RUNX2, BMP4, BMP2, TGFβ1, EGF, and SMAD6 indicating a possible role of these genes in dental morphology.

Clinical relevance

Polymorphisms in odontogenesis-related genes may be involved in dental morphology enabling a prediction of permanent TS variability. The knowledge regarding genes involved in TS might impact the personalized dental treatment, considering that patients’ genetic profile would soon be introduced into clinical practice to improve patient management.

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Acknowledgments

The authors want to thank Lucas Ramazzotto for providing technical assistance.

Funding

This work was supported by the São Paulo Research Foundation (FAPESP) (funding number: 2015/06866-5) and individual scholarships (FAPESP and CAPES).

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Authors and Affiliations

  1. School of Health Sciences, Positivo University, 5300 Professor Pedro Viriato Parigot de Souza Street, Campo Comprido, Curitiba, PR, 81280-330, Brazil

    Jennifer Tsi Gerber, Katheleen Miranda dos Santos, Bruna Karas Brum, Maria Fernanda Pivetta Petinati & Michelle Nascimento Meger

  2. Department of Stomatology, School of Dentistry, Federal University of Parana, 632 Prefeito Lothario Meissner Avenue, Curitiba, PR, 80210-170, Brazil

    Delson João da Costa & Rafaela Scariot

  3. Department of Medical and Anatomical Sciences, College of Ostheopathic Medicine of the Pacific, Western Universitiy, 615 E 3rd St, Pomona, CA, 91766, USA

    Mohammed Elsalanty

  4. Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida do Café s/n – Campus da USP, Ribeirao Preto, SP, 14040-904, Brazil

    Erika Calvano Küchler

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Contributions

J.T.G contributed to formal analyses, investigation, data curation, writing original draft, reviewing, and editing the manuscript; K.M.S. and B.K.B contributed to investigation and data curation; M.F.P contributed to data visualization; M.N.M performed the investigation and data curation; D.J.C. contributed to investigation and supervision; M.E contributed to writing, reviewing, and editing the manuscript; E.C.K contributed to study design, resources, funding acquisition, laboratorial analysis, and reviewing the manuscript; R.S. contributed to conceptualization, methodology, project administration, funding acquisition, and reviewing the manuscript. All authors gave final approval and agreed to account for all aspects of the work.

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Correspondence to Rafaela Scariot.

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Gerber, J.T., dos Santos, K.M., Brum, B.K. et al. Odontogenesis-related candidate genes involved in variations of permanent teeth size. Clin Oral Invest 25, 4481–4494 (2021). https://doi.org/10.1007/s00784-020-03760-0

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