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Effect of ozone to remineralize initial enamel caries: in situ study

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Abstract

Objectives

Effect of ozonated water in remineralizing artificially created initial enamel caries was investigated using laser fluorescence and polarized light microscopy in an in situ study.

Materials and methods

Teeth specimens (buccal sections) were immersed in 5-ml solution of 2 mM CaCl2, 2 mM NaH2P04, and 50 mM CH3COOH at pH of 4.55 for 5 h in an incubator at 37° to create subsurface demineralization. After which, they were randomly allocated into one of the following remineralization regimens: ozone (ozonated water 0.1 mg/l and 10 % nano-hydroxyapatite paste, AclaimTM), without ozone (only 10 % nano-hydroxyapatite paste, AclaimTM), and control (subjects’ saliva alone). Specimens were embedded in acrylic retainers worn by orthodontic patients throughout the 21-day study duration and constantly exposed to their saliva. Laser fluorescence was recorded for all the specimens at baseline, after demineralization, and remineralization using DIAGNOdent, and the results were validated using polarized microscopic examination. The results were analyzed using repeated measures, one-way ANOVA with post hoc multiple comparisons.

Results

Reduced DIAGNOdent scores and greater depth of remineralization following application of ozonated water and nano-hydroxyapatite were found compared to those of the without ozone and control groups (P < 0.001), and the ozone-treated group exhibited maximum remineralization under the polarized light microscopy.

Conclusion

Ozonated water can be considered an effective agent in reversing the initial enamel caries alongside with nano-hydroxyapatite compared to nano-hydroxyapatite alone and saliva.

Clinical relevance

Ozone water can be used to remineralize incipient carious lesions, and it enhances the remineralizing potential of nano-hydroxyapatite thereby preventing the tooth from entering into the repetitive restorative cycle.

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Authors and Affiliations

  1. Department of Public Health Dentistry, Thai Moogambigai Dental College and Hospital, Chennai, India

    S. R. Samuel

  2. Department of Oral and Maxillofacial Pathology, Manipal College of Dental Sciences, Mangalore, India

    S. Dorai

  3. Department of Public Health Dentistry, Government Dental College, Nagpur, India

    S. G. Khatri

  4. Department of Public Health Dentistry, Sri Krishna Institute of Medical and Dental Sciences, Karad, India

    S. T. Patil

Authors
  1. S. R. Samuel
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  2. S. Dorai
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  3. S. G. Khatri
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  4. S. T. Patil
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Corresponding author

Correspondence to S. R. Samuel.

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Conflict of interest

The authors declare that they have no competing interests.

Funding

No funding was received for this study.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and national research committee (ICMR) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Samuel, S.R., Dorai, S., Khatri, S.G. et al. Effect of ozone to remineralize initial enamel caries: in situ study. Clin Oral Invest 20, 1109–1113 (2016). https://doi.org/10.1007/s00784-016-1710-x

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  • DOI: https://doi.org/10.1007/s00784-016-1710-x

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