Abstract
Objectives
The aim of this study was the evaluation of microleakage in class V cavities using micro-CT imaging and dye penetration.
Materials and methods
Sixty class V cavities were prepared, randomly divided into five groups (Adper Single Bond Plus (ASB), ExciTE (EX), iBond TE (ITE), Optibond Solo Plus (OSP), Prime & Bond NT (PB)) and restored with Venus Diamond. After thermocycling and dye penetration test with 50 wt% ammoniacal silver nitrate, eight samples per group were sectioned longitudinally and evaluated under a coaxial incident light microscope (enamel (E) and dentin (D) measurement in mm). The remaining samples were analyzed by counting voids in the coronal and cervical adhesive areas by means of micro-CT.
Results
In dentin, no statistically significant difference in dye penetration was found. In enamel, OSP showed significant higher microleakage than all other adhesives (p < 0.001). Groups ITE, ASB, PB, and EX showed a significantly lower penetration depth in E than in D (p < 0.001). The results of the micro-CT examination in dentin are contrary to the findings of the microleakage evaluation. Regarding enamel, only group PB showed significant more voids than EX in enamel (p < 0.02). Within the adhesive groups, PB showed significantly more voids in E than in D (p < 0.04).
Conclusions
This test method must be optimized by developing a new leakage tracer for a better differentiation between hard tissues and dental materials.
Clinical relevance
The micro-CT is not yet a vulnerable tool to evaluate the marginal integrity of resin composites.
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References
Pecie R, Krejci I, Garcia-Godoy F, Bortolotto T (2011) Noncarious cervical lesions—a clinical concept based on the literature review. Part 1: prevention. Am J Dent 24:49–56
Ferracane JL, Mitchem JC (2003) Relationship between composite contraction stress and leakage in class V cavities. Am J Dent 16:239–243
Davidson CL, De Gee AJ, Feilzer AJ (1984) The competition between the composite dentin bond strength and polymerization contraction stress. J Dent Res 63:1396–1399
Kidd EA, Beighton D (1996) Prediction of secondary caries around tooth-colored restorations: a clinical and microbiological study. J Dent Res 75:1942–1946
Alomari QD, Barrieshi-Nusair K, Ali M (2011) Effect of C-factor and LED curing mode on microleakage of class V resin composite restorations. Eur J Dent 5:400–408
Atoui JA, Chinelatti MA, Palma-Dibb RG, Corona SA (2010) Microleakage in conservative cavities varying the preparation method and surface treatment. J Appl Oral Sci 18:421–425
Lührs AK, Guhr S, Schilke R, Borchers L, Geurtsen W, Günay H (2008) Shear bond strength of self-etch adhesives to enamel with additional phosphoric acid etching. Oper Dent. doi:10.2341/07-63
Mousavinasab SM, Atai M, Alavi B (2011) To compare the microleakage among experimental adhesives containing nanoclay fillers after the storages of 24 hours and 6 months. Open Dent J. doi:10.2174/1874210601105010052
Peumans M, De Munck J, Van Landuyt K, Lambrechts P, Van Meerbeek B (2007) Five-year clinical effectiveness of a two-step self-etching adhesive. J Adhes Dent 9:7–10
Geerts S, Bolette A, Seidel L, Guéders A (2012) An in vitro evaluation of leakage of two etch and rinse and two self-etch adhesives after thermocycling. Int J Dent. doi:10.1155/2012/852841
Luque-Martinez I, Muñoz MA, Mena-Serrano A, Hass V, Reis A, Loguercio AD (2015) Effect of EDTA conditioning on cervical restorations bonded with a self-etch adhesive: a randomized double-blind clinical trial. J Dent 43:1175–1183
Hickel R, Peschke A, Tyas M, Mjör I, Bayne S, Peters M, Hiller KA, Randall R, Vanherle G, Heintze SD (2010) FDI world dental federation—clinical criteria for the evaluation of direct and indirect restorations. Update and clinical examples. J Adhes Dent 12(4):259–272
Faye B, Sarr M, Bane K, Aidara AW, Niang O, Kane AW (2015) One-year clinical evaluation of the bonding effectiveness of a one-step self-etch adhesive in noncarious cervical lesion therapy, Int J Dent doi:org/10.1155/2015/984065
Baracco B, Fuentes MV, Ceballos L (2015) Five-year clinical performance of a silorane- vs a methacrylate-based composite combined with two different adhesive approaches. Clin Oral Invest. doi:10.1007/s00784-015-1591-4
Fiasconaro J, Sherman HA (1952) A quantitative comparative evaluation of the sealing properties of the self-curing resins. J Am Dent Assoc 31:503–508
Kidd EA (1976) Microleakage: a review. J Dent 4:199–206
Fabianelli A, Pollington S, Davidson CL, Cagidiaco MC, Goracci C (2007) The relevance of micro-leakage studies. Int Dent SA 9:64–74
Hickel R, Peschke A, Tyas M, Mjör I, Bayne S, Peters M, Hiller KA, Randall R, Vanherle G, Heintze SD (2010) FDI World Dental Federation—clinical criteria for the evaluation of direct and indirect restorations. Update and clinical examples. J Adhes Dent 12:259–272
Heintze S, Forjanic M, Cavalleri A (2008) Microleakage of class II restorations with different tracers—comparison with SEM quantitative analysis. J Adhes Dent 10:259–267
Mente J, Ferk S, Dreyhaupt J, Deckert A, Legner M, Staehle HJ (2010) Assessment of different dyes used in leakage studies. Clin Oral Investig. doi:10.1007/s00784-009-0299-8
Youngson CC, Jones JC, Manoque M, Smith IS (1998) In vitro penetration by tracers used in microleakage studies. Int Endod J 31:90–99
Zhao X, Li SB, Gu L, Li Y (2014) Detection of marginal leakage of class V restorations in vitro by micro-computed tomography. Oper Dent. doi:10.2341/12-182-L
Sun J, Lin-Gibson S (2008) X-ray microcomputed tomography for measuring polymerization shrinkage of polymeric dental composites. Dent Mater 24:228–234
De Santis R, Mollica F, Prisco D, Rengo S, Ambrosio L, Nicolais L (2005) A 3D analysis of mechanically stressed dentin-adhesive-composite interfaces using X-ray micro-CT. Biomaterials 26:257–270
Van Meerbeek B, Yoshihara K, Yoshida Y, Mine A, De Munck J, Van Landuyt KL (2011) State of the art of self-etch adhesives. Dent Mater. doi:10.1016/j.dental.2010.10.023
Zeiger DN, Sun J, Schumacher GE, Lin-Gibson S (2009) Evaluation of dental composite shrinkage and leakage in extracted teeth using X-ray microcomputed tomography. Dent Mater 25:1213–1220
Kakaboura A, Rahiotis C, Watts D, Silikas N, Eliades G (2007) 3D-marginal adaptation versus setting shrinkage in light-cured microhybrid resin composites. Dent Mater 23:272–278
Vinay S, Shivanna V (2010) Comparative evaluation of microleakage of fifth, sixth, and seventh generation dentin bonding agents: an in vitro study. J Conserv Dent 13:136–140
Peumans M, Kanumilli P, De Munck J, Van Landuyt K, Lambrechts P, Van Meerbeek B (2005) Clinical effectiveness of contemporary adhesives: a systematic review of current clinical trails. Dent Mater 21:864–881
Heintze SD, Ruffieux C, Rousson V (2010) Clinical performance of cervical restorations—a meta-analysis. Dent Mater 26:993–1000
Peumans M, De Munck J, Mine A, Van Meerbeek B (2014) Clinical effectiveness of contemporary adhesives for the restoration of non-carious cervical lesions. A systematic review. Dent Mater 30:1089–1103
International Organization for Standardization. Dentistry—testing of adhesion to tooth structure. ISO/TS 11405: 2013
Hilton TJ (1998) Can modern restorative procedures and materials reliably seal cavities? In vitro investigations. Part 1. Am J Dent 15:198–210
Costa JF, Siqueira WL, Loguercio AD, Reis A, Ed O, Alves CM, Bauer JR, Grande RH (2011) Characterization of aqueous silver nitrate solution for leakage tests. J Appl Oral Sci 19:254–259
Hammesfahr PD, Huang CT, Shaffer SE (1987) Microleakage and bond strength of resin restorations with various bonding agents. Dent Mater 3:194–199
Tay FR, Pashley DH (2003) Water treeing—a potential mechanism for degradation of dentin adhesives. Am J Dent 16:6–12
Swain MV, Xue J (2009) State of the art of micro-CT applications in dental research. Int J Oral Sci 1:177–188
Heintze SD (2007) Systematic reviews: I. The correlation between laboratory tests on marginal quality and bond strength. II. The correlation between marginal quality and clinical outcome. J Adhes Dent 9(Suppl 1):77–10639
Carrera AC, Lan C, Escobar-Sanabria D, Li Y, Rudney J, Aparicio C, Fok A (2015) The use of micro-CT with image segmentation to quantify leakage in dental restorations. Dent Mater 31:382–390
Kierklo A, Tabor Z, Petryniak R, Dohnalik M, Jaworska M (2014) Application of microcomputed tomography for quantitative analysis of dental root canal obturations. Postepy Hig Med Dosw. doi:10.5604/17322693.1095271
Kierklo A, Tabor Z, Pawińska M, Jaworska M (2015) A microcomputed tomography-based comparison of root canal filling quality following different instrumentation and obturation techniques. Med Princ Pract. doi:10.1159/000368307
Wolf M, Küpper K, Reimann S, Bourauel C, Frentzen M (2014) 3D analyses of interface voids in root canals filled with different sealer materials in combination with warm gutta-percha technique. Clin Oral Invest. doi:10.1007/s00784-013-0970-y
Neves AA, Jaecques S, Van Ende A, Cardoso MV, Coutinho E, Lührs A-K, Zicari F, Van Meerbeek B (2014) 3D-microleakage assessment of adhesive interface: exploratory findings by μCT. Dent Mater 30:799–807
Ishii K, Matsuo M, Hoshi N, Takahashi SS, Kawamata R, Kimoto K (2015) Effect of ultraviolet irradiation of implant surface on progression of periimplantitis—a pilot study in dogs. Implant Dent [EPub ahead of print]
Borba M, Cesar PF, Griggs JA, Bona ÁD (2011) Adaptation of all-ceramics fixed partial dentures. Dent Mater. doi:10.1016/j.dental.2011.08.004
Borba M, Miranda WG jr, Cesar PF, Griggs JA, Bona ÁD (2013) Evaluation of the adaptation of zirconia-based fixed partial dentures using micro-CT technology. Braz Oral Res doi:10.1590/S1806-83242013000500003
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The authors declare that they have no competing interests.
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All procedures performed in this study were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments.
The use of extracted human teeth was approved by the ethical committee of our university (no. 2092–2013). The manuscript does not contain clinical studies or patient data.
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Silke Jacker-Guhr and Gabriela Ibarra contributed equally to this work.
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Jacker-Guhr, S., Ibarra, G., Oppermann, L.S. et al. Evaluation of microleakage in class V composite restorations using dye penetration and micro-CT. Clin Oral Invest 20, 1709–1718 (2016). https://doi.org/10.1007/s00784-015-1676-0
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DOI: https://doi.org/10.1007/s00784-015-1676-0