Skip to main content

Advertisement

Log in

Evaluation of microleakage in class V composite restorations using dye penetration and micro-CT

  • Original Article
  • Published:
Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Objectives

The aim of this study was the evaluation of microleakage in class V cavities using micro-CT imaging and dye penetration.

Materials and methods

Sixty class V cavities were prepared, randomly divided into five groups (Adper Single Bond Plus (ASB), ExciTE (EX), iBond TE (ITE), Optibond Solo Plus (OSP), Prime & Bond NT (PB)) and restored with Venus Diamond. After thermocycling and dye penetration test with 50 wt% ammoniacal silver nitrate, eight samples per group were sectioned longitudinally and evaluated under a coaxial incident light microscope (enamel (E) and dentin (D) measurement in mm). The remaining samples were analyzed by counting voids in the coronal and cervical adhesive areas by means of micro-CT.

Results

In dentin, no statistically significant difference in dye penetration was found. In enamel, OSP showed significant higher microleakage than all other adhesives (p < 0.001). Groups ITE, ASB, PB, and EX showed a significantly lower penetration depth in E than in D (p < 0.001). The results of the micro-CT examination in dentin are contrary to the findings of the microleakage evaluation. Regarding enamel, only group PB showed significant more voids than EX in enamel (p < 0.02). Within the adhesive groups, PB showed significantly more voids in E than in D (p < 0.04).

Conclusions

This test method must be optimized by developing a new leakage tracer for a better differentiation between hard tissues and dental materials.

Clinical relevance

The micro-CT is not yet a vulnerable tool to evaluate the marginal integrity of resin composites.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig 1
Fig 2
Fig. 3
Fig 4

Similar content being viewed by others

References

  1. Pecie R, Krejci I, Garcia-Godoy F, Bortolotto T (2011) Noncarious cervical lesions—a clinical concept based on the literature review. Part 1: prevention. Am J Dent 24:49–56

    PubMed  Google Scholar 

  2. Ferracane JL, Mitchem JC (2003) Relationship between composite contraction stress and leakage in class V cavities. Am J Dent 16:239–243

    PubMed  Google Scholar 

  3. Davidson CL, De Gee AJ, Feilzer AJ (1984) The competition between the composite dentin bond strength and polymerization contraction stress. J Dent Res 63:1396–1399

    Article  PubMed  Google Scholar 

  4. Kidd EA, Beighton D (1996) Prediction of secondary caries around tooth-colored restorations: a clinical and microbiological study. J Dent Res 75:1942–1946

    Article  PubMed  Google Scholar 

  5. Alomari QD, Barrieshi-Nusair K, Ali M (2011) Effect of C-factor and LED curing mode on microleakage of class V resin composite restorations. Eur J Dent 5:400–408

    PubMed  PubMed Central  Google Scholar 

  6. Atoui JA, Chinelatti MA, Palma-Dibb RG, Corona SA (2010) Microleakage in conservative cavities varying the preparation method and surface treatment. J Appl Oral Sci 18:421–425

    Article  PubMed  Google Scholar 

  7. Lührs AK, Guhr S, Schilke R, Borchers L, Geurtsen W, Günay H (2008) Shear bond strength of self-etch adhesives to enamel with additional phosphoric acid etching. Oper Dent. doi:10.2341/07-63

    PubMed  Google Scholar 

  8. Mousavinasab SM, Atai M, Alavi B (2011) To compare the microleakage among experimental adhesives containing nanoclay fillers after the storages of 24 hours and 6 months. Open Dent J. doi:10.2174/1874210601105010052

    PubMed  PubMed Central  Google Scholar 

  9. Peumans M, De Munck J, Van Landuyt K, Lambrechts P, Van Meerbeek B (2007) Five-year clinical effectiveness of a two-step self-etching adhesive. J Adhes Dent 9:7–10

    PubMed  Google Scholar 

  10. Geerts S, Bolette A, Seidel L, Guéders A (2012) An in vitro evaluation of leakage of two etch and rinse and two self-etch adhesives after thermocycling. Int J Dent. doi:10.1155/2012/852841

    PubMed  PubMed Central  Google Scholar 

  11. Luque-Martinez I, Muñoz MA, Mena-Serrano A, Hass V, Reis A, Loguercio AD (2015) Effect of EDTA conditioning on cervical restorations bonded with a self-etch adhesive: a randomized double-blind clinical trial. J Dent 43:1175–1183

    Article  PubMed  Google Scholar 

  12. Hickel R, Peschke A, Tyas M, Mjör I, Bayne S, Peters M, Hiller KA, Randall R, Vanherle G, Heintze SD (2010) FDI world dental federation—clinical criteria for the evaluation of direct and indirect restorations. Update and clinical examples. J Adhes Dent 12(4):259–272

    PubMed  Google Scholar 

  13. Faye B, Sarr M, Bane K, Aidara AW, Niang O, Kane AW (2015) One-year clinical evaluation of the bonding effectiveness of a one-step self-etch adhesive in noncarious cervical lesion therapy, Int J Dent doi:org/10.1155/2015/984065

  14. Baracco B, Fuentes MV, Ceballos L (2015) Five-year clinical performance of a silorane- vs a methacrylate-based composite combined with two different adhesive approaches. Clin Oral Invest. doi:10.1007/s00784-015-1591-4

    Google Scholar 

  15. Fiasconaro J, Sherman HA (1952) A quantitative comparative evaluation of the sealing properties of the self-curing resins. J Am Dent Assoc 31:503–508

    Google Scholar 

  16. Kidd EA (1976) Microleakage: a review. J Dent 4:199–206

    Article  PubMed  Google Scholar 

  17. Fabianelli A, Pollington S, Davidson CL, Cagidiaco MC, Goracci C (2007) The relevance of micro-leakage studies. Int Dent SA 9:64–74

    Google Scholar 

  18. Hickel R, Peschke A, Tyas M, Mjör I, Bayne S, Peters M, Hiller KA, Randall R, Vanherle G, Heintze SD (2010) FDI World Dental Federation—clinical criteria for the evaluation of direct and indirect restorations. Update and clinical examples. J Adhes Dent 12:259–272

  19. Heintze S, Forjanic M, Cavalleri A (2008) Microleakage of class II restorations with different tracers—comparison with SEM quantitative analysis. J Adhes Dent 10:259–267

    PubMed  Google Scholar 

  20. Mente J, Ferk S, Dreyhaupt J, Deckert A, Legner M, Staehle HJ (2010) Assessment of different dyes used in leakage studies. Clin Oral Investig. doi:10.1007/s00784-009-0299-8

    PubMed  Google Scholar 

  21. Youngson CC, Jones JC, Manoque M, Smith IS (1998) In vitro penetration by tracers used in microleakage studies. Int Endod J 31:90–99

    Article  PubMed  Google Scholar 

  22. Zhao X, Li SB, Gu L, Li Y (2014) Detection of marginal leakage of class V restorations in vitro by micro-computed tomography. Oper Dent. doi:10.2341/12-182-L

    PubMed  Google Scholar 

  23. Sun J, Lin-Gibson S (2008) X-ray microcomputed tomography for measuring polymerization shrinkage of polymeric dental composites. Dent Mater 24:228–234

    Article  PubMed  Google Scholar 

  24. De Santis R, Mollica F, Prisco D, Rengo S, Ambrosio L, Nicolais L (2005) A 3D analysis of mechanically stressed dentin-adhesive-composite interfaces using X-ray micro-CT. Biomaterials 26:257–270

    Article  PubMed  Google Scholar 

  25. Van Meerbeek B, Yoshihara K, Yoshida Y, Mine A, De Munck J, Van Landuyt KL (2011) State of the art of self-etch adhesives. Dent Mater. doi:10.1016/j.dental.2010.10.023

    Google Scholar 

  26. Zeiger DN, Sun J, Schumacher GE, Lin-Gibson S (2009) Evaluation of dental composite shrinkage and leakage in extracted teeth using X-ray microcomputed tomography. Dent Mater 25:1213–1220

    Article  PubMed  PubMed Central  Google Scholar 

  27. Kakaboura A, Rahiotis C, Watts D, Silikas N, Eliades G (2007) 3D-marginal adaptation versus setting shrinkage in light-cured microhybrid resin composites. Dent Mater 23:272–278

    Article  PubMed  Google Scholar 

  28. Vinay S, Shivanna V (2010) Comparative evaluation of microleakage of fifth, sixth, and seventh generation dentin bonding agents: an in vitro study. J Conserv Dent 13:136–140

    Article  PubMed  PubMed Central  Google Scholar 

  29. Peumans M, Kanumilli P, De Munck J, Van Landuyt K, Lambrechts P, Van Meerbeek B (2005) Clinical effectiveness of contemporary adhesives: a systematic review of current clinical trails. Dent Mater 21:864–881

    Article  PubMed  Google Scholar 

  30. Heintze SD, Ruffieux C, Rousson V (2010) Clinical performance of cervical restorations—a meta-analysis. Dent Mater 26:993–1000

    Article  PubMed  Google Scholar 

  31. Peumans M, De Munck J, Mine A, Van Meerbeek B (2014) Clinical effectiveness of contemporary adhesives for the restoration of non-carious cervical lesions. A systematic review. Dent Mater 30:1089–1103

    Article  PubMed  Google Scholar 

  32. International Organization for Standardization. Dentistry—testing of adhesion to tooth structure. ISO/TS 11405: 2013

  33. Hilton TJ (1998) Can modern restorative procedures and materials reliably seal cavities? In vitro investigations. Part 1. Am J Dent 15:198–210

    Google Scholar 

  34. Costa JF, Siqueira WL, Loguercio AD, Reis A, Ed O, Alves CM, Bauer JR, Grande RH (2011) Characterization of aqueous silver nitrate solution for leakage tests. J Appl Oral Sci 19:254–259

    Article  PubMed  PubMed Central  Google Scholar 

  35. Hammesfahr PD, Huang CT, Shaffer SE (1987) Microleakage and bond strength of resin restorations with various bonding agents. Dent Mater 3:194–199

    Article  PubMed  Google Scholar 

  36. Tay FR, Pashley DH (2003) Water treeing—a potential mechanism for degradation of dentin adhesives. Am J Dent 16:6–12

    PubMed  Google Scholar 

  37. Swain MV, Xue J (2009) State of the art of micro-CT applications in dental research. Int J Oral Sci 1:177–188

    Article  PubMed  PubMed Central  Google Scholar 

  38. Heintze SD (2007) Systematic reviews: I. The correlation between laboratory tests on marginal quality and bond strength. II. The correlation between marginal quality and clinical outcome. J Adhes Dent 9(Suppl 1):77–10639

    PubMed  Google Scholar 

  39. Carrera AC, Lan C, Escobar-Sanabria D, Li Y, Rudney J, Aparicio C, Fok A (2015) The use of micro-CT with image segmentation to quantify leakage in dental restorations. Dent Mater 31:382–390

    Article  PubMed  PubMed Central  Google Scholar 

  40. Kierklo A, Tabor Z, Petryniak R, Dohnalik M, Jaworska M (2014) Application of microcomputed tomography for quantitative analysis of dental root canal obturations. Postepy Hig Med Dosw. doi:10.5604/17322693.1095271

    Google Scholar 

  41. Kierklo A, Tabor Z, Pawińska M, Jaworska M (2015) A microcomputed tomography-based comparison of root canal filling quality following different instrumentation and obturation techniques. Med Princ Pract. doi:10.1159/000368307

    PubMed  Google Scholar 

  42. Wolf M, Küpper K, Reimann S, Bourauel C, Frentzen M (2014) 3D analyses of interface voids in root canals filled with different sealer materials in combination with warm gutta-percha technique. Clin Oral Invest. doi:10.1007/s00784-013-0970-y

    Google Scholar 

  43. Neves AA, Jaecques S, Van Ende A, Cardoso MV, Coutinho E, Lührs A-K, Zicari F, Van Meerbeek B (2014) 3D-microleakage assessment of adhesive interface: exploratory findings by μCT. Dent Mater 30:799–807

    Article  PubMed  Google Scholar 

  44. Ishii K, Matsuo M, Hoshi N, Takahashi SS, Kawamata R, Kimoto K (2015) Effect of ultraviolet irradiation of implant surface on progression of periimplantitis—a pilot study in dogs. Implant Dent [EPub ahead of print]

  45. Borba M, Cesar PF, Griggs JA, Bona ÁD (2011) Adaptation of all-ceramics fixed partial dentures. Dent Mater. doi:10.1016/j.dental.2011.08.004

    Google Scholar 

  46. Borba M, Miranda WG jr, Cesar PF, Griggs JA, Bona ÁD (2013) Evaluation of the adaptation of zirconia-based fixed partial dentures using micro-CT technology. Braz Oral Res doi:10.1590/S1806-83242013000500003

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to S. Jacker-Guhr.

Ethics declarations

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

All procedures performed in this study were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments.

The use of extracted human teeth was approved by the ethical committee of our university (no. 2092–2013). The manuscript does not contain clinical studies or patient data.

Additional information

Silke Jacker-Guhr and Gabriela Ibarra contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jacker-Guhr, S., Ibarra, G., Oppermann, L.S. et al. Evaluation of microleakage in class V composite restorations using dye penetration and micro-CT. Clin Oral Invest 20, 1709–1718 (2016). https://doi.org/10.1007/s00784-015-1676-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00784-015-1676-0

Keywords

Navigation