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Effect of pulverized natural bone mineral on regeneration of three-wall intrabony defects. A preclinical study

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Abstract

Aims

The objective of this study is to evaluate the effects of a paste-like bone substitute material with easy handling properties and improved mechanical stability on periodontal regeneration of intrabony defects in dogs.

Materials and methods

Mandibular and maxillary first and third premolars were extracted, and three-wall intrabony defects were created on second and fourth premolars. After a healing period of 3 months, acute type defects were filled with a paste-like formulation of deproteinized bovine bone mineral (DBBM) (particle size, 0.125–0.25 mm) in a collagenous carrier matrix (T1), pulverized DBBM (particle size, 0.125–0.25 mm) without the carrier (T2), or Bio-Oss® granules (particle size, 0.25–1.00 mm) as control (C). All defects were covered with a Bio-Gide® membrane. The dogs were sacrificed after 12 weeks, and the specimens were analyzed histologically and histometrically.

Results

Postoperative healing of all defects was uneventful, and no histological signs of inflammation were observed in the augmented and gingival regions. New cementum, new periodontal ligament, and new bone were observed in all three groups. The mean vertical bone gain was 3.26 mm (T1), 3.60 mm (T2), and 3.81 mm (C). That of new cementum was 2.25 mm (T1), 3.88 mm (T2), and 3.53 mm (C). The differences did not reach statistical significance. The DBBM particles were both incorporated in new bone and embedded in immature bone marrow.

Conclusions

The results of this preclinical study showed that the 0.125–0.25-mm DBBM particles in a powder or paste formulation resulted in periodontal regeneration comparable to the commercially available DBBM. Osteoconductivity, in particular, was not affected by DBBM size or paste formulation.

Clinical relevance

The improved handling properties of the paste-like bone substitute consisting of small DBBM particles embedded in a collagen-based carrier hold promise for clinical applications.

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Acknowledgments

The authors wish to thank Ms. Monika Aeberhard, Ms. Thuy-Trang Nguyen, and Mr. David Reist for the histological preparation of the specimens and Mr. Walter Bürgin for performing the statistical analyses. The study was funded by Geistlich Pharma, Wolhusen, Switzerland.

Conflict of interest

The authors report no conflicts of interest related to this study.

Author information

Authors and Affiliations

  1. Department of Periodontology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, 3010, Bern, Switzerland

    A. Ivanovic, D. D. Bosshardt, R. Gruber & A. Sculean

  2. Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Bern, Switzerland

    D. D. Bosshardt & R. Gruber

  3. Robert K. Schenk Laboratory of Oral Histology, School of Dental University of Bern, Bern, Switzerland

    D. D. Bosshardt

  4. Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany

    I. Mihatovic & F. Schwarz

  5. Laboratory of Oral Cell Biology, School of Dental University of Bern, Bern, Switzerland

    R. Gruber

Authors
  1. A. Ivanovic
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  2. D. D. Bosshardt
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  3. I. Mihatovic
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  4. F. Schwarz
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  5. R. Gruber
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  6. A. Sculean
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Correspondence to A. Sculean.

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Ivanovic, A., Bosshardt, D.D., Mihatovic, I. et al. Effect of pulverized natural bone mineral on regeneration of three-wall intrabony defects. A preclinical study. Clin Oral Invest 18, 1319–1328 (2014). https://doi.org/10.1007/s00784-013-1089-x

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  • DOI: https://doi.org/10.1007/s00784-013-1089-x

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