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Pulpal responses after application of current adhesive systems to deep cavities

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Abstract

The aim of the present study was to evaluate comparatively the pulpal tissue reactions of four adhesive systems placed in experimental cavities of healthy dog’s teeth. Class V cavities with a mean value of remaining dentin thickness (RDT) ranging between 0.55 ± 0.30–0.68 ± 0.38 mm were prepared. The cavities were treated with the following adhesive systems: Etch and Prime 3.0 (EP), Single Bond (SB), Clearfil SE Bond (CSE), and Prompt L-Pop (PLP). The pulpal tissue responses to dentin adhesives were assessed histopathologically at postoperative periods of 7, 21, and 65 days, and the results were subjected to statistical analysis. A significantly greater adverse inflammatory response was observed with the materials EP and PLP, while a significantly lesser degree of disorganization in the odontoblastic zone was found with the materials SB and CSE, in the postoperative period of 65 days. In addition, a thicker predentin zone was observed where SB material was applied. Application of the selected adhesive systems to non-exposed cavities, with an RDT, which ranged between the above-mentioned rates, was correlated with slight to moderate inflammation and odontoblast reduction depending on the materials used as well as upon the RDT.

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References

  1. About I, Murray PE, Franquin J-C, Remusat M, Smith AJ (2001) Pulpal inflammation responses following non-carious class V restorations. Oper Dent 26:336–342

    PubMed  Google Scholar 

  2. Bouillaguet S, Wataha JC, Hanks CT, Ciucchi B, Holz J (1996) In vitro cytotoxicity and dentin permeability of HEMA. J Endod 22:244–248

    Article  PubMed  Google Scholar 

  3. Chan K, Tay F, King N, Imazato S, Pashley D (2003) Bonding of mild self-etching primers/adhesives to dentin with thick smear layers. Am J Dent 16:340–346

    PubMed  Google Scholar 

  4. Costa CAS,Vaerten MA, Edwards CA, Hanks CT (1999) Cytotoxicity of current dental adhesive systems on immortalized odontoblast cell line MDPC-23. Dent Mater 15:434–441

    Article  PubMed  Google Scholar 

  5. Costa CAS, Hebling J, Hanks CT (2000) Current status of pulp capping with dentin adhesive systems: a review. Dent Mater 16:188–199

    Article  PubMed  Google Scholar 

  6. Costa CAS, Nascimento ABL, Teixeira HM (2002) Response of human pulps following acid conditioning and application of a bond agent in deep cavities. Dent Mater 18:543–551

    Article  Google Scholar 

  7. Frankenberger R, Krämer N, Petschelt A (2000) Technique sensitivity of dentin bonding: effect of application mistakes on bond strength and marginal adaptation. Oper Dent 25:324–330

    PubMed  Google Scholar 

  8. Frankenberger R, Perdigăo J, Rosa B, Lopes M (2001) “No bottle-bottle” vs “multi-bottle” dentin adhesives: a microtensile bond strength and morphological study. Dent Mater 17:373–380

    Article  PubMed  Google Scholar 

  9. Gwinnett AJ, Tay F (1998) Early and intermediate time response of the dental pulp to an acid etch technique in vivo. Am J Dent 11:S35–S44

    PubMed  Google Scholar 

  10. Hanks CT, Strawn RR, Wataha JC, Craig RG (1991) Cytotoxic effects of resin components on cultured mammalian fibroblasts. J Dent Res 70:1450–1455

    PubMed  Google Scholar 

  11. Hashimoto M, Ohno H, Kaga M,Endo K, Sano H, Oguchi H (2000) In vivo degradation of resin–dentin bonds in humans over 1–3 years. J Dent Res 79:1385–1391

    PubMed  Google Scholar 

  12. Hebling J, Giro EMA, Costa GAS (1999) Human pulp response after an adhesive system application in deep cavities. J Dent 27:557–564

    Article  PubMed  Google Scholar 

  13. Inoue S, Van Meerbeek B, Vargas M, Yoshida Y, Lambrechts P, Vanherle G (1999) Adhesion mechanism of self-etching adhesives. Am J Dent 10:131–175

    Google Scholar 

  14. Inoue S, van Meerbeek B, Vargas M, Yoshida Y, Lambrechts P, Vanherle G (2000) Adhesion mechanism of self-etching adhesives. In: Tagami J, Toledano M, Prati C (eds) Advanced adhesive dentistry. 3rd International Kuraray Symposium, 1999, Cranada, Spain. Crafiche Erredue, Crimido (Como), Italy, pp 131–148

  15. Inoue S, Vargas MA, Abe Y, Yoshida Y, Lambrechts P, Vanherle G, Sano H, Van Meerbeek B (2001) Micro-tensile bond strength of eleven contemporary modern adhesives to dentine. J Adhes Dent 3:237–245

    PubMed  Google Scholar 

  16. Lesot H, Begue-Kirn C, Kubler JD, Meyer JM, Smith AJ, Cassidy N, Ruch JV (1993) Experimental induction of odontoblast differentiation and stimulation during reparative processes. Cells Mater 3:201–217

    Google Scholar 

  17. Murray PE, Lumley PJ, Ross HF, Smith AJ (2000) Tooth Slice organ culture for cytotoxicity assessment of dental materials. Biomaterials 21:1711–1721

    Article  PubMed  Google Scholar 

  18. Murray PE, About I, Lumley PJ, Franquin J-C, Remusat M, Smith AJ (2002) Cavity remaining dentin thickness and pulpal activity. Am J Dent 15:41–46

    PubMed  Google Scholar 

  19. Murray PE, Smith AJ, Windsor LJ, Mjör IA (2003) Remaining dentine thickness and human pulp responses. Int Endod J 36:33–43

    Article  PubMed  Google Scholar 

  20. Pashley EL, Agee KA, Pashley DH, Tay FR (2002) Effects of one versus two applications of an unfilled, all-in-one adhesive on dentine bonding. J Dent 30:83–90

    Article  PubMed  Google Scholar 

  21. Sano H, Yoshikawa T, Pereira PNR, Kanemura N, Morigami M, Tagami J, Pashley DH (1999) Long-term durability of dentin bonds made with a self-etching primer. J Dent Res 78:906–911

    PubMed  Google Scholar 

  22. Stanley HR (1968) Design for a human pulp study. Part 1. Oral Surg Oral Med Oral Pathol 25:633–647

    Article  PubMed  Google Scholar 

  23. Tay FR, Gwinnet JA, Wei SHY (1998) Relation between water content in acetone/alcohol-based primer and interfacial ultrastructure. J Dent 26:147–156

    Article  PubMed  Google Scholar 

  24. Tay FR, Sano H, Carvalho R, Pashley E, Pashley DH (2000a) An ultrastructural study of the influence of acidity of self-etching primers and smear layer thickness on bonding to intact dentin. J Adhes Dent 2:83–98

    PubMed  Google Scholar 

  25. Tay FR, Carvalho R, Sano H, Pashley DH (2000b) Effect of smear layers on the bonding of a self -etching primer in dentin. J Adhes Dent 2:99–116

    PubMed  Google Scholar 

  26. Tziafas D, Koliniotou-Koumpia E, Tziafa C, Papadimitriou S (2007) Effects of a new antibacterial adhesive on the repair capacity of the pulp-dentine complex in infected teeth. Int Endod J 40:58–66

    Article  PubMed  Google Scholar 

  27. Van Meerbeek B, Yoshida Y, Inoue S, Vargas S, Abe Y, Fukuda R, Okazaki M, Lambrechts P, Vanherle G (2000) Bonding mechanism and micro-tensile bond strength of a 4-MET-based self-etching adhesive. J Dent Res 79(special issue):249 (abstract #845)

    Google Scholar 

  28. Van Meerbeek B, Vargas S, Inoue S, Yoshida Y, Peumans M, Lambrechts P, Vanherle G (2001) Adhesives and cements to promote preservation dentistry. In: Proceeding from the international symposium on management alternatives for the carious lesions. Oper Dent 26(Suppl 6):119–144

    Google Scholar 

  29. Van Meerbeek B, De Munck J, Yoshida Y, Inoue S, Vargas M, Vijay P, Van Landuyt K, Lambrechts P, Vanherle G (2003) Adhesion to enamel and dentin: current status and future challenges. Buonocore memorial lectured. Oper Dent 28:215–235

    PubMed  Google Scholar 

  30. Wang Y, Spencer P (2004) Physicochemical interactions at the interfaces between self-etch adhesive systems and dentine. J Dent 32:567–579

    Article  PubMed  Google Scholar 

  31. Wang Y, Spencer P (2005) Continuing etching of an all-in-one adhesive in wet dentin tubules. J Dent Res 84(4):350–354

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The present study was partially co-funded by the European Union-European Social Fund and the National Fund Pythagoras-EPEAEK II.

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Correspondence to Eugenia Koliniotou-Koumpia.

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Koliniotou-Koumpia, E., Papadimitriou, S. & Tziafas, D. Pulpal responses after application of current adhesive systems to deep cavities. Clin Oral Invest 11, 313–320 (2007). https://doi.org/10.1007/s00784-007-0121-4

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  • DOI: https://doi.org/10.1007/s00784-007-0121-4

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