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DNA binding and oxidative DNA damage induced by a quercetin copper(II) complex: potential mechanism of its antitumor properties

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Abstract

The interaction of a quercetin copper(II) complex with DNA was investigated using UV–vis spectra, fluorescence measurement, viscosity measurement, agarose gel electrophoresis, and thiobarbituric acid reactive substances assay. The results indicate that the quercetin copper(II) complex can promote the cleavage of plasmid DNA, producing single and double DNA strand breaks, and intercalate into the stacked base pairs of DNA. Moreover, the complex can induce oxidative DNA damage involving generation of reactive oxygen species such as H2O2 and Cu(I)OOH. In addition, the cytotoxicity experiments carried out with A549 cells confirmed its apoptosis-inducing activity. And we also demonstrate that the levels of survivin protein expression in A549 cells decreased, and that relative activity of caspase-3 increased significantly after treatment with the complex. So our results suggest that the antitumor mechanism of the quercetin copper(II) complex involves not only its oxidative DNA damage with generation of reactive oxygen species but also its specific interaction with DNA.

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Acknowledgments

This research was financially supported by the Science and Technology Project of Education Commission of Chongqing (grant no. KJ051503) and a China Postdoctoral Science Foundation funded project.

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Authors and Affiliations

  1. Bioengineering College, Chongqing University, No. 174 Shapingba Main Street, 400030, Chongqing, China

    Jun Tan, Bochu Wang & Liancai Zhu

  2. Department of Life Science and Chemistry, Chongqing Education College, 400067, Chongqing, China

    Jun Tan

  3. Mechanical Resource and Environment College, Post-doctoral Research Center of Mechanics, Chongqing University, 400030, Chongqing, China

    Jun Tan

Authors
  1. Jun Tan
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  2. Bochu Wang
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  3. Liancai Zhu
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Correspondence to Jun Tan or Bochu Wang.

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Tan, J., Wang, B. & Zhu, L. DNA binding and oxidative DNA damage induced by a quercetin copper(II) complex: potential mechanism of its antitumor properties. J Biol Inorg Chem 14, 727–739 (2009). https://doi.org/10.1007/s00775-009-0486-8

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  • DOI: https://doi.org/10.1007/s00775-009-0486-8

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