Abstract
The investigation was to determine the effect of camel milk fermented with Limosilactobacillus fermentum KGL4 (MTCC 25515) on ACE-inhibiting, anti-inflammatory, and diabetes-preventing properties and also to release the novel peptides with antidiabetic and anti-hypertensive attributes with molecular interaction studies. Growth conditions were optimised on the basis of total peptide production by inoculating the culture in camel milk at different rates (1.5, 2.0, and 2.5%) along with different incubation periods (12, 24, 36, and 48 h). However, after 48 h of fermentation with a 2.5% rate of inoculum, the highest proteolytic activity was obtained. Reverse phase high-pressure liquid chromatography (RP-HPLC) was used to calculate the % Rpa from permeates of 3 kDa and 10 kDa fractions. Molecular weight distributions of fermented and unfermented camel milk protein fractions were compared using SDS-PAGE. Spots obtained from 2D gel electrophoresis were separated on the basis of pH and molecular weight. Spots obtained from 2D gel were digested with trypsin, and the digested samples were subjected to RP-LC/MS for the generation of peptide sequences. The inhibition of tumour necrosis factor alpha, interleukin-6, and interleukin-1 during fermentation was studied using RAW 264.7 macrophages. In the study, fermented camel milk with KGL4 (CMKGL4) inhibited LPS-induced nitric oxide (NO) production and pro-inflammatory cytokine production (TNF-α, IL-6, and IL-1β) by the murine macrophages. The results showed that the peptide structures (YLEELHRLNK and YLQELYPHSSLKVRPILK) exhibited considerable binding affinity against hPAM and hMGA during molecular interaction studies.
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Data availability
The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability
Not Applicable.
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Conceptualization: PS, SH; Data curation: PS, AS, RM, MB, KKK, SH; Formal analysis: CK, SH, AS, MS, KKK; Funding acquisition: SH; Investigation: CK, RM, AS, BB, MB, KKK, SH; Methodology: CK, RM, AS, AKR, SP; Project administration: SH, KKK, AS; Resources: SH, AS, MB, ZL, KKK; Software: AS, CK, SH, SP; Supervision: SH, AS, KKK; Validation: CK, SD, SH, KKK; Visualization: SD, AS, CK, RM; Writing-original draft: PS, BB, SP, AKR; Writing-review and editing: BB, RM, SH, AS, ZL, KKK.
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Shukla, P., Sakure, A., Basaiawmoit, B. et al. Molecular binding mechanism and novel antidiabetic and anti-hypertensive bioactive peptides from fermented camel milk with anti-inflammatory activity in raw macrophages cell lines. Amino Acids 55, 1621–1640 (2023). https://doi.org/10.1007/s00726-023-03335-9
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DOI: https://doi.org/10.1007/s00726-023-03335-9