Abstract
We developed a hollow fiber centrifugal ultrafiltration (HFCF-UF) method to study the change of plasma levels of free glycine (Gly) in patients with acute ischemic stroke (AIS). Twenty-four patients with AIS confirmed by diffusion-weighted imaging (DWI) were enrolled. During the study period, the patients did not receive any supplemental amino acids therapy that could affect the obtained results. Our results showed that although AIS patients adopted different methods of treatment (thrombolytic and non-thrombolytic), the clinical NIHSS score of AIS showed a downward trend whereas Gly concentration showed increased trend. Moreover, plasma free Gly concentration was positively correlated with ASPECTS score. The correlation between Gly levels and infarct volume showed a statistical significance. That is to say, higher Gly level predicted smaller infarct size. Thus, the change of free Gly level in plasma could be considered as a potential biomarker of AIS.
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This study was supported by the Natural Science Foundation of Hebei Province [grant numbers H2021206299].
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The study conception and design, acquisition of data, and first draft of the manuscript were performed by HW. The authors XN and WD helped in the acquisition of data, material preparation, respectively. WQ and LX helped in the collection of clinical plasma sample. YJ and LX are responsible for the guidance of the method part and the clinical part, respectively. All authors contributed to the final version of the manuscript.
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The study protocol was approved by the ethics committee of The Second Hospital of Hebei Medical University, in accordance with the ethical guidelines of the 1975 Declaration of Helsinki. Written consent was obtained from all the patients. Research Ethics Committee Ethical Approval number: 2022-R318.
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Wang, H., Ni, X., Dong, W. et al. Accurately quantified plasma free glycine concentration as a biomarker in patients with acute ischemic stroke. Amino Acids 55, 385–402 (2023). https://doi.org/10.1007/s00726-023-03236-x
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DOI: https://doi.org/10.1007/s00726-023-03236-x