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Gene expression profiling of hybridoma cells after bursal-derived bioactive factor BP5 treatment

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Abstract

Bursa of Fabricius is the acknowledged vital humoral immune system for B cell differentiation and antibody production. To study the molecular mechanism underlying the effect of bursal-derived BP5, we used gene microarray to analyze the genomic expression profiling of BP5-treated hybridoma cells. BP5 exhibited an immunomodulatory effect on antibody production in hybridoma cells and induced alterations in the gene expression profiles related to the immune-related biological processes, such as T cell activation and proliferation, B cell activation, B cell-mediated immunity, and cytokines cytokine production involved in immune response. In addition, 26 biological pathways associated with immunomodulatory functions were regulated in BP5-treated hybridoma cells, in which p53 signal pathway played an important role in antitumor. Among these regulated genes, 12 differentially expressed genes were verified by qRT-PCR. The activation of p53 activity by BP5 was further confirmed by p53 luciferase reporter assay and p53 expression. Our data revealed that bursal-derived BP5 could regulate various immune-related cellular processes, including antitumor factor p53 signal pathway, perhaps partially accounting for the reported immunomodulatory roles and novel antiproliferation on tumor cells functions of bursal-derived bioactive factor BP5.

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Acknowledgments

This work was supported by Grant No. 200803020 and No. 201203082 from the National Agriculture Special Research Project for Non-Profit Trades, Ministry of Agriculture and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and the National Special Research Programs for Cultivating New Transgenic Organisms, Ministry of Agriculture (NO. 2009ZX08006-007B). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. And the gene microarray was performed by KangChen Bio-tech (China, Shanghai).

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The authors declared no conflict of interest.

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Authors and Affiliations

  1. Division of Key Lab of Animal Disease Diagnosis and Immunology of China’s Department of Agriculture, College of Veterinary Medicine, Nanjing Agriculture University, Nanjing, 210095, China

    Xiu L. Feng, Rui B. Cao, Bin Zhou, De Y. Li, Yuan P. Zhang, Ke Liu, Xiao D. Liu, Xin F. Li & Pu Y. Chen

  2. Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China

    Qing T. Liu

  3. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, 200241, China

    Jian C. Wei, Ya F. Qiu & Zhi Y. Ma

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  1. Xiu L. Feng
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Feng, X.L., Liu, Q.T., Cao, R.B. et al. Gene expression profiling of hybridoma cells after bursal-derived bioactive factor BP5 treatment. Amino Acids 43, 2443–2456 (2012). https://doi.org/10.1007/s00726-012-1323-x

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