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A bursal pentapeptide (BPP-I), a novel bursal-derived peptide, exhibits antiproliferation of tumor cell and immunomodulator activity

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Abstract

The bursa of Fabricius (BF) is the central humoral immune organ unique to birds. Here, we isolated a novel bursal pentapeptide I (BPP-I), LGPGP, from BF. BPP-I could play inhibition effect on MCF-7 but not on CEF or Vero cell proliferation in vitro, and enhance antitumor factor p53 protein expression. Also, BPP-I stimulated antibody production in a dose-dependent manner in hybridoma cell. Furthermore, BPP-I could induce various immune responses in mice immunization experiments, including increase antibody production and cytokines IL-4 and IFN-γ level, and induce T-cell immunophenotyping. These results suggest that BPP-I is a potential immunomodulator of antitumor and immunity. The study could provide some novel insights on the probable candidate reagent for the antitumor and immune improvement.

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Acknowledgements

This work was supported by the National Agriculture Special Research Project for Non-Profit Trades, Ministry of Agriculture (No. 200803020). Also, we thank Elsevier Language Editing Services (https://languageediting.elsevier.com/) for professional language editing service in the preparation of this manuscript.

Conflict of interest

The authors declared no conflict of interest.

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Authors and Affiliations

  1. Division of Key Lab of Animal Disease Diagnosis and Immunology of China’s Department of Agriculture, College of Veterinary Medicine, Nanjing Agriculture University, Nanjing, 210095, People’s Republic of China

    Xiu L. Feng, Rui B. Cao, Bin Zhou, Fang Q. Wang, Wen L. Deng, Yu Zhang, Hassan Ishag & Pu Y. Chen

  2. Nanjing Tianbang Bio-Industry Co. Ltd, Nanjing, People’s Republic of China

    Qing T. Liu

  3. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, 200241, People’s Republic of China

    Ya F. Qiu & Zhi Y. Ma

  4. Division of National Veterinary Biological Medicine Engineering Research Center, Nanjing, People’s Republic of China

    Qi S. Zheng

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  1. Xiu L. Feng
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  2. Qing T. Liu
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  3. Rui B. Cao
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  4. Bin Zhou
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  5. Fang Q. Wang
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  8. Yu Zhang
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  9. Hassan Ishag
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  10. Zhi Y. Ma
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  12. Pu Y. Chen
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Correspondence to Pu Y. Chen.

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Feng, X.L., Liu, Q.T., Cao, R.B. et al. A bursal pentapeptide (BPP-I), a novel bursal-derived peptide, exhibits antiproliferation of tumor cell and immunomodulator activity. Amino Acids 42, 2215–2222 (2012). https://doi.org/10.1007/s00726-011-0961-8

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  • DOI: https://doi.org/10.1007/s00726-011-0961-8

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