Abstract
Sulfur (S) is an essential macronutrient required by plants. Plants absorb and transport S through sulfate transporters (SULTRs). In this study, we cloned 8 SULTR genes (CsSULTR1;1/1;2/2;1/3;1/3;2/3;3/3;5/4;1) from tea plant (Camellia sinensis), all of which contain a typical sulfate transporter and antisigma factor antagonist (STAS) conserved domain. Phylogenetic tree analysis further divided the CsSULTRs into four main groups. Many cis-acting elements related to hormones and environmental stresses were found within the promoter sequence of CsSULTRs. Subcellular localization results showed that CsSULTR4;1 localized in the vacuolar membrane and that other CsSULTRs localized to the cellular membrane. The tissue-specific expression of the 8 CsSULTR genes showed different expression patterns during the active growing period and dormancy period. In particular, the expression of CsSULTR1;1 was highest in the roots, but that of CsSULTR1;2 was lowest in the dormancy period. The expression of CsSULTR1;1/1;2/2;1/3;2 was stimulated under different concentrations of selenium (Se) and S; moreover, CsSULTR1;2/2;1/3;3/3;5 was upregulated in response to different valences of Se.
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Abbreviations
- S:
-
Sulfur
- Se:
-
Selenium
- SULTRs:
-
Sulfate transporters
- CsSULTRs:
-
Sulfate transporters of tea plant (Camellia sinensis)
- GSH:
-
Glutathione
- h-GSH:
-
Homo-GSH
- PCs:
-
Phytochelatins
- TMDs:
-
Transmembrane domains
- STAS:
-
Sulfate transporter and antisigma factor antagonist
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Funding
This work was supported by the Central Public-interest Scientific Institution Basal Research Fund (1610212016001), the Agricultural Sciences and Technology Innovation Program of Chinese Academy of Agricultural Sciences (ASTIP), the Special R & D Program Project of Chinese Academy of Se-enriched Industry (2019QCY-1.2), and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences (CAAS-XTCX20190025-7).
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JMZ, XH, and LW conceived and designed the experiments. HZ, JJZ, and LG performed the experiments. HZ, JJZ, and YW analyzed the data and prepared some figures and tables. NL, XH, and HR provided advice on the analysis and interpretation of the data. HZ, JJZ, and YW prepared some figures and tables. HZ and XH wrote the paper. All authors read and approved the final manuscript.
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Zhang, H., Hao, X., Zhang, J. et al. Genome-wide identification of SULTR genes in tea plant and analysis of their expression in response to sulfur and selenium. Protoplasma 259, 127–140 (2022). https://doi.org/10.1007/s00709-021-01643-z
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DOI: https://doi.org/10.1007/s00709-021-01643-z