Abstract
In this study, we have shown the applicability of electroporation and hygromycin B as a convenient selectable marker for stable nuclear transformation of Coccomyxa subellipsoidea C-169. Since it is the first sequenced eukaryotic microorganism from polar environment, this offers unique opportunities to study adaptation mechanisms to cold.
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Abbreviations
- BBM:
-
Bold’s Basal Medium
- BFP:
-
Blue fluorescent protein
- hygB:
-
Hygromycin B
- basta:
-
DL-phosphinothricin
- str:
-
Streptomycin
- kan:
-
Kanamycin
- gen:
-
Gentamycin
- neo:
-
Neomycin
- chl:
-
Chloramphenicol
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Kania, K., Zienkiewicz, M. & Drożak, A. Stable transformation of unicellular green alga Coccomyxa subellipsoidea C-169 via electroporation. Protoplasma 257, 607–611 (2020). https://doi.org/10.1007/s00709-019-01447-2
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DOI: https://doi.org/10.1007/s00709-019-01447-2