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Reactive oxygen species and nitric oxide are involved in polyamine-induced growth inhibition in wheat plants

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Abstract

Polyamines (PAs) produce H2O2 and nitric oxide (NO) during their normal catabolism and modulate plant growth and development. To explore the biochemical basis of PAs-induced growth inhibition in Triticum aestivum L seedlings, we examined the role of O2·-, H2O2 or NO in shoot and root development. Although all PA treatments resulted in a variable reduction of root and shoot elongation, spermine (Spm) caused the greater inhibition in a similar way to that observed with the NO donor, sodium nitroprusside (SNP). In both cases, O2·- production was completely blocked whereas H2O2 formation was high in the root apex under SNP or Spm treatments. Catalase recovered root and shoot growth in SNP but not in Spm-treated plants, revealing the involvement of H2O2 in SNP-root length reduction. The addition of the NO scavenger, cPTIO, restored root length in SNP- or Spm-treated plants, respectively, and partially recovered O2·- levels, compared to the plants exposed to PAs or SNP without cPTIO. A strong correlation was observed between root growth restoration and O2·- accumulation after treating roots with SNP + aminoguanidine, a diamine oxidase inhibitor, and with SNP + 1,8-diaminoctane, a polyamine oxidase inhibitor, confirming the essential role of O2·- formation for root growth and the importance of the origin and level of H2O2. The differential modulation of wheat growth by PAs through reactive oxygen species or NO is discussed.

Polyamines, nitric oxide and ROS interaction in plants during plant growth

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Abbreviations

AG:

Aminoguanidine

1,8-DO:

1,8-diaminoctane

SNP:

Sodium nitroprusside

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Funding

This work was supported by grants from the Universidad de Buenos Aires, Argentina (UBACYT 20020130100178BA), from Consejo Nacional de Investigaciones Científicas y Técnicas (PIP 266, IQUIFIB-CONICET, Argentina) and ANPCyT (Agencia Nacional de Promoción Científica y Tecnológica, Argentina). L.R is a Universidad de Buenos Aires fellow, A.V is an CONICET fellow, and M.D.G and M.P.B are career investigators from CONICET.

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  1. Facultad de Farmacia y Bioquímica, Departamento de Química Biológica, Cátedra de Química Biológica Vegetal, Universidad de Buenos Aires, Junín 956 1º piso, C1113AAC, Buenos Aires, Argentina

    Laura Recalde, María D. Groppa & María Patricia Benavides

  2. IQUIFIB-CONICET, Junín 956, C1113AAC, Buenos Aires, Argentina

    Analía Vázquez, María D. Groppa & María Patricia Benavides

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  1. Laura Recalde
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  2. Analía Vázquez
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  3. María D. Groppa
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  4. María Patricia Benavides
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Contributions

L.R and A.V performed experimental work; L.R, M.D.G and M.P.B performed data analysis, L.R and M.P.B oversaw project planning; L.R, M.D.G and M.P.B wrote the paper.

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Correspondence to María Patricia Benavides.

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Supplemental Fig. 1

Root growth of wheat plants after 5 d in the respective treatment as it is indicated in the picture: 0.1 mM SNP; 1 mM Put, Spd or Spm, with the addition of ROS, NO or PAs modulators: 1000 U ml-1 CAT; 200 μM cPTIO; 0.5 mM AG; 0.3 mM 1,8 OD; 2 mM CaCl2. The figure is representative of five different experiments (PPTX 3674 kb)

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Recalde, L., Vázquez, A., Groppa, M.D. et al. Reactive oxygen species and nitric oxide are involved in polyamine-induced growth inhibition in wheat plants. Protoplasma 255, 1295–1307 (2018). https://doi.org/10.1007/s00709-018-1227-z

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