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Alterations in the sugar metabolism and in the vacuolar system of mesophyll cells contribute to the desiccation tolerance of Haberlea rhodopensis ecotypes

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Abstract

Haberlea rhodopensis belongs to the small group of resurrection plants having the unique ability to survive desiccation to air dry state retaining most of its chlorophyll content and then resume normal function upon rehydration. It prefers the shady valleys and northward facing slopes of limestone ridges in mountain zones with high average humidity. Nevertheless, it can be found rarely on rocks directly exposed to the sunlight, without the coverage of the canopy. In the present study, we follow the alterations in the subcellular organization of mesophyll cells and sugar metabolism upon desiccation of shade and sun H. rhodopensis plants. Composition and content of soluble carbohydrates during desiccation and rehydration were different in plants grown below the trees or on the sunny rocks. Sucrose, however, was dominating in both ecotypes. The amount of starch grains in chloroplasts was inversely related to that of sugars. Concomitantly with these changes, the number of vacuoles was multiplied in the cells. This can be explained by the development of small (secondary) vacuoles peripherally in the cytoplasm, rather than by the fragmentation of the single vacuole, proposed earlier in the literature. Accordingly, the centripetal movement of chloroplasts and other organelles may be a result of the dynamic changes in the vacuolar system. Upon rehydration, the inner vacuoles enlarged and the organelles returned to their normal position.

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Acknowledgments

We would like to thank to Csilla Gergely for technical assistance. This work was supported by the Bilateral Research Agreement of Bulgarian and Hungarian Academies of Sciences and the Bilateral Research Agreement of Bulgarian Academy of Sciences and National Council of Research, Italy. Á. Solti was also supported by the Bolyai János Research Scholarship of the Hungarian Academy of Sciences (BO/00207/15/4).

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Authors and Affiliations

  1. Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., Bl. 21, BG-1113, Sofia, Bulgaria

    K. Georgieva & G. Mihailova

  2. Institute of Biometeorology, National Research Council, Via Gobetti 101, 40129, Bologna, Italy

    F. Rapparini & G. Bertazza

  3. Department of Plant Physiology and Molecular Plant Biology, Institute of Biology, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary

    É. Sárvári & Á. Solti

  4. Department of Plant Anatomy, Institute of Biology, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary

    Á. Keresztes

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  1. K. Georgieva
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  2. F. Rapparini
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  3. G. Bertazza
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  4. G. Mihailova
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  5. É. Sárvári
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  6. Á. Solti
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Correspondence to Á. Keresztes.

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Georgieva, K., Rapparini, F., Bertazza, G. et al. Alterations in the sugar metabolism and in the vacuolar system of mesophyll cells contribute to the desiccation tolerance of Haberlea rhodopensis ecotypes. Protoplasma 254, 193–201 (2017). https://doi.org/10.1007/s00709-015-0932-0

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  • DOI: https://doi.org/10.1007/s00709-015-0932-0

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