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Cumulative effect of nitrogen and sulphur on Brassica juncea L. genotypes under NaCl stress

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Abstract

In the present study, N and S assimilation, antioxidant enzymes activity, and yield were studied in N and S-treated plants of Brassica juncea (L.) Czern. & Coss. (cvs. Chuutki and Radha) under salt stress. The treatments were given as follows: (1) NaCl90 mM+N0S0 mg kg-1 sand (control), (2) NaCl90 mM+N60S0 mg kg-1 sand, (3) NaCl90 mM+N60S20 mg kg-1 sand, (4) NaCl90 mM+N60S40 mg kg-1 sand, and (5) NaCl90 mM+N60S60 mg kg-1 sand. The combined application of N (60 mg kg−1 sand) and S (40 mg kg−1 sand) proved beneficial in alleviating the adverse effect of salt stress on growth attributes (shoot length plant−1, fresh weight plant−1, dry weight plant−1, and area leaf−1), physio-biochemical parameters (carbonic anhydrase activity, total chlorophyll, adenosine triphosphate-sulphurylase activity, leaf N, K and Na content, K/Na ratio, activity of nitrate reductase, nitrite reductase, glutamine synthetase, glutamate synthase, catalase, superoxide dismutase, ascorbate peroxidase and glutathione reductase, and content of glutathione and ascorbate), and yield attributes (pods plant−1, seeds pod−1, and seed yield plant−1). Therefore, it is concluded that combined application of N and S induced the physiological and biochemical mechanisms of Brassica. The stimulation of antioxidant enzymes activity and its synergy with N and S assimilation may be one of the important mechanisms that help the plants to tolerate the salinity stress and resulted in an improved yield.

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Acknowledgment

We thank anonymous reviewers for their valuable suggestions and critical reading of the manuscript and Professor Adel Salah Abdul-Jabbar (director of Attracting Outstanding Faculty and Researchers Program) for providing the opportunity to work in the Department of Botany and Microbiology, King Saud University, Saudi Arabia.

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  1. Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia

    Manzer H. Siddiqui & Mohamed H. Al-Whaibi

  2. Plant Physiology Section, Department of Botany, Aligarh Muslim University, Aligarh, UP, 202002, India

    Firoz Mohammad & M. Masrooor A. Khan

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  1. Manzer H. Siddiqui
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Siddiqui, M.H., Mohammad, F., Khan, M.M.A. et al. Cumulative effect of nitrogen and sulphur on Brassica juncea L. genotypes under NaCl stress. Protoplasma 249, 139–153 (2012). https://doi.org/10.1007/s00709-011-0273-6

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