Summary.
We have previously reported the construction of a recombinant feline herpesvirus type 1 (FHV-1), designated C7301ddlTK-gag, expressing the Gag precursor protein of feline immunodeficiency virus (FIV). In this study, we report the construction of a further recombinant FHV-1 (ddlTK(gBp)-gag) which carries an FHV-1 gB promoter sequence upstream of the FIV gag gene of C7301ddlTK-gag. Strong expression of the FIV Gag protein by ddlTK(gBp)-gag was confirmed by immunoblot analyses and enzyme-linked immunosorbent assays. Although C7301ddlTK-gag and ddlTK(gBp)-gag failed to induce anti-FIV Gag antibodies in cats, we confirmed the infectivity and stability of these recombinants in cats.
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Received January 14, 2000 Accepted August 4, 2000
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Sato, E., Miyazawa, T., Nishimura, Y. et al. Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus. Arch. Virol. 146, 379–387 (2001). https://doi.org/10.1007/s007050170182
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DOI: https://doi.org/10.1007/s007050170182