Skip to main content

Advertisement

SpringerLink
Log in

Recombinant human antibodies specific for hepatitis C virus proteins

  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Human antibodies to hepatitis C virus core, NS4A and NS3 were cloned in a prokaryotic vector and expressed as soluble Fab fragments and as phage-displayed Fabs. The recombinant Fabs were shown to be a suitable tool for immunohistochemistry, since they recognize the cognate antigen expressed in mammalian cells. The nucleotide sequence of the cDNA for the variable domains of these antibodies was determined and the V-gene usage was derived. On the basis of the deduced amino acid sequence, a structural model of the V domains of the Fabs was constructed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Istituto di Ricerche di Biologia Molecolare P. Angeletti (IRBM), Pomezia (Roma), Italy, Italy

    G. Esposito, V. Morea, E. Scarselli, N. La Monica & C. Traboni

  2. Istituto di Clinica delle Malattie Infettive, I.R.C.C.S. Policlinico S. Matteo, University of Pavia, Pavia, Italy, Italy

    A. Cerino & M. U. Mondelli

Authors
  1. G. Esposito
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. Morea
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Scarselli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Cerino
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. U. Mondelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. N. La Monica
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. C. Traboni
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Esposito, G., Morea, V., Scarselli, E. et al. Recombinant human antibodies specific for hepatitis C virus proteins. Arch. Virol. 142, 601–610 (1997). https://doi.org/10.1007/s007050050106

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s007050050106

Search

Navigation