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Molecular and biological characterization of a novel citrus tristeza virus isolate that causes severe symptoms in Citrus junos cv. Ziyangxiangcheng

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Abstract

The complete genomic sequence of a novel citrus tristeza virus (CTV) isolate, CT91-A1, from Orah tangor grafted on Citrus junos cv. Ziyangxiangcheng rootstock in China was determined by transcriptome sequencing. Sequence alignments showed that isolate CT91-A1 shared 83.3 to 95.5% nucleotide sequence identity with extant CTV genotypes at the whole-genome level, with the highest similarity to the S1 genotype. Phylogenetic analysis revealed that CT91-A1 clustered in a unique subclade with the S1 genotype. Isolate CT91-A1 induced severe stem pitting in Mexican lime and C. junos cv. Ziyangxiangcheng and moderate stem pitting in Guanximiyou pummelo and Duncan grapefruit. It was successfully transmitted by Aphis citricidus, and it can potentially cause significant damage to the citrus industry in China.

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Fig. 1

Data availability statement

The datasets generated during and/or analysed during the current study are available in the NCBI repository, [https://www.ncbi.nlm.nih.gov/].

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Acknowledgements

This work was partially supported by the China Agriculture Research System of MOF and MARA, Chongqing and Frontier Technology (cstc2019jcyj-msxmX0557).

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Author notes

  1. Yangyang Qin and Yingjie Liu contributed equally to this work.

Authors and Affiliations

  1. Citrus Research Institute, Southwest University, Chongqing, 400712, China

    Yangyang Qin, Yingjie Liu, Jinfa Zhao, Jiajun Wang, Xiao Ye & Yan Zhou

  2. Citrus Pest Detect Program CCTEA, Tulare, CA, USA

    Subhas Hajeri

Authors
  1. Yangyang Qin
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  2. Yingjie Liu
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  3. Jinfa Zhao
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  4. Subhas Hajeri
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  5. Jiajun Wang
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  6. Xiao Ye
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  7. Yan Zhou
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Correspondence to Yan Zhou.

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Qin, Y., Liu, Y., Zhao, J. et al. Molecular and biological characterization of a novel citrus tristeza virus isolate that causes severe symptoms in Citrus junos cv. Ziyangxiangcheng. Arch Virol 168, 59 (2023). https://doi.org/10.1007/s00705-022-05644-y

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  • DOI: https://doi.org/10.1007/s00705-022-05644-y

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