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A novel victorivirus isolated from the tobacco spot blight fungus Stagonosporopsis cucurbitacearum in China

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Abstract

Stagonosporopsis cucurbitacearum is an important plant-pathogenic fungus that causes stem and leaf blight diseases in a variety of crops. Here, we report the characterization of a novel victorivirus, tentatively named “Stagonosporopsis cucurbitacearum victorivirus 1” (ScVV-1), isolated from the S. cucurbitacearum isolate M-7. The ScVV-1 genome is 5,165 bp in length with a predicted GC content of 60.1% and contains two large open reading frames (ORF 1 and ORF2) encoding putative proteins that share significant sequence similarity with coat proteins (CPs) and RNA-dependent RNA polymerases (RdRps) of mycoviruses of the family Totiviridae. The ScVV-1 RdRp appears to be translated using a stop-initiation pentanucleotide UAAUG sequence. Phylogenetic analysis based on CP and RdRp amino acid (aa) sequences both indicated that ScVV-1 belongs to the genus Victorivirus in the family Totiviridae. To our knowledge, this is the first full-length genome sequence of a victorivirus infecting S. cucurbitacearum.

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Data availability

The datasets generated during the current study are available in the GenBank database with accession number ON563739.

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Funding

This study was supported by the fund of Innovation projects of Zhengzhou Tobacco Research Institute of CNTC (122021CR0030) and China Tobacco Jiangsu Industry Co., Ltd (122021DG0570).

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Authors and Affiliations

  1. Key Laboratory of Eco-environment and Leaf Tobacco Quality, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, Henan, China

    Mengmeng Yang, Guiyao Wang, Wenjun Mu, Jianhua Guo, Jiaqin Xi, Liwei Hu, Kexin Liang & Jizhen Song

  2. China Tobacco Jiangsu Industry Co., Ltd, Nanjing, 210000, Jiangsu, China

    Qiang Xu & Zongyu Hu

Authors
  1. Mengmeng Yang
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  2. Guiyao Wang
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  3. Wenjun Mu
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  4. Jianhua Guo
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  5. Jiaqin Xi
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  6. Liwei Hu
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  7. Kexin Liang
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  8. Qiang Xu
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  9. Zongyu Hu
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  10. Jizhen Song
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Contributions

Mengmeng Yang performed the experiments and wrote the manuscript. Guiyao Wang participated in some trials and selected the manuscript format. Mengmeng Yang and Guiyao Wang contributed equally to this work. Wenjun Mu, Jianhua Guo, Jiaqin Xi, Liwei Hu, and Kexin Liang were involved in the design of the investigation. Qiang Xu helped collected leaf samples to obtain fungal isolates. Zongyu Hu and Jizhen Song designed and supervised the investigation.

Corresponding authors

Correspondence to Zongyu Hu or Jizhen Song.

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The authors have no relevant financial or non-financial interests to disclose.

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Handling Editor: Ioly Kotta-Loizou.

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Yang, M., Wang, G., Mu, W. et al. A novel victorivirus isolated from the tobacco spot blight fungus Stagonosporopsis cucurbitacearum in China. Arch Virol 167, 2845–2850 (2022). https://doi.org/10.1007/s00705-022-05618-0

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  • DOI: https://doi.org/10.1007/s00705-022-05618-0

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