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Complete genome sequence of a novel citrus virus with characteristics of members of the family Tymoviridae

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Abstract

A novel positive-stranded RNA virus provisionally named "citrus virus C" (CVC) was discovered in citrus trees displaying mottling symptoms. Its genome comprises 7,215 nucleotides (nt), excluding the 3’ poly(A) tail, and contains two open reading frames (ORFs) that encode a replication-associated polyprotein (RP) and a putative coat protein (CP). The CVC genome contains a 16-nt ‘marafibox’, which is highly conserved in most viruses belonging to the genus Marafivirus of the same family. Sequence analysis suggested that the virus is most closely related to grapevine Red Globe virus (GRGV), which is yet to be officially classified in the family Tymoviridae. The sequence identities between CVC and GRGV in the whole genome (50.7%, nt) and CP (49.4% for amino acid, and 53.9% for nt) are lower than the thresholds (80% in the genome and 90% in the CP) for species demarcation in the family. Therefore, it is legitimate to propose that CVC is a member of new species in the family Tymoviridae.

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Acknowledgements

This research was supported by the National Key R&D Program of China (2019YFD1001800), the National Natural Science Foundation of China (32072389), the Innovation Program for Chongqing's Overseas Returnees (cx2019013), and the Overseas Expertise Introduction Project for Discipline Innovation (111 Center) (B18044). We thank the editor and two anonymous reviewers for their constructive comments and suggestions.

I have read and abided by the statement of ethical standards for manuscripts submitted to Archives of Virology.

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Authors and Affiliations

  1. National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China

    Liu Yang, Song Zhang, Shiqiang Mei, Qiyan Liu, Yan Zhou & Mengji Cao

  2. Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China

    Liu Yang, Song Zhang, Shiqiang Mei, Qiyan Liu, Yan Zhou & Mengji Cao

  3. National Germplasm Resources Laboratory, USDA-ARS, Beltsville, MD, 20705, USA

    Ruhui Li

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  1. Liu Yang
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  2. Song Zhang
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  3. Shiqiang Mei
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  4. Qiyan Liu
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  5. Yan Zhou
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  7. Mengji Cao
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Correspondence to Mengji Cao.

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Yang, L., Zhang, S., Mei, S. et al. Complete genome sequence of a novel citrus virus with characteristics of members of the family Tymoviridae. Arch Virol 166, 2055–2058 (2021). https://doi.org/10.1007/s00705-021-05082-2

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  • DOI: https://doi.org/10.1007/s00705-021-05082-2

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