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Retrospective detection and phylogenetic analysis of pseudorabies virus in dogs in China

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Abstract

Pseudorabies virus (PRV), the causative agent of Aujeszky’s disease, has gained increased attention in China in recent years as a result of a recent outbreak of pseudorabies. The causative agent has a wide spectrum of hosts, including pigs, cattle, sheep, dogs, cats, bats, bears, and even some avian species. Although dog-related cases of pseudorabies have been reported regularly, many cases are overlooked, and few PRV strains are isolated because death occurs rapidly after PRV infection and veterinarians often do not test for PRV in dogs. Here, we performed a retrospective detection of PRV in dogs from July 2017 to December 2018. We found that PRV (including gE-deleted strains, classical strains, and variant strains) is prevalent in dogs regardless of season and region and that the epidemic PRV strains in dogs share high sequence similarity with gC and gE genes of swine epidemic strains and commercial vaccine strains. Collectively, our findings underscore the importance of PRV surveillance in dogs, which is beneficial for understanding the epidemiology of PRV in dogs and assists in efforts aimed at effectively controlling this disease.

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Acknowledgements

This work was funded by the National Key Research and Development Program of China (2016YFD0501003 &2 017YFD0501603) and the Natural Science Foundation of Guangdong Province (2018A030310206). We thank Dr. Yungfu Chang at Cornell University for critical reading of the manuscript.

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Author notes

  1. Lu Tu, Jiamin Lian and Yanling Pang contributed equally.

Authors and Affiliations

  1. Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, College of Animal Science, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Jiamin Lian, Yanling Pang & Wencheng Lin

  2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China

    Lu Tu, Cun Liu & Shangjin Cui

Authors
  1. Lu Tu
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  2. Jiamin Lian
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  3. Yanling Pang
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  4. Cun Liu
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  5. Shangjin Cui
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  6. Wencheng Lin
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Contributions

LT and JL collected samples from southern China, isolated three PRV strains, and sequenced the gE and gC genes; YP sequenced and analyzed the genomic sequences of three PRV strains and performed the phylogenic analysis of the gE genes; CL collected samples from northern China; LT, JL, SC, and WL wrote the original draft. All authors revised the main manuscript and cooperated for its improvement.

Corresponding authors

Correspondence to Shangjin Cui or Wencheng Lin.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The Committee on the Ethics of Animal Experiments at South China Agricultural University approved the animal experiment protocols (approval ID: SYXK-2014-0136). Permission was issued for the field studies, and all of the owners of dogs gave informed consent to conduct the study at this site.

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Handling Editor: Akbar Dastjerdi.

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Tu, L., Lian, J., Pang, Y. et al. Retrospective detection and phylogenetic analysis of pseudorabies virus in dogs in China. Arch Virol 166, 91–100 (2021). https://doi.org/10.1007/s00705-020-04848-4

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  • DOI: https://doi.org/10.1007/s00705-020-04848-4

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