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The complete genome sequence of a divergent grapevine virus I isolate naturally infecting grapevine in Greece

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Abstract

A significant number of new members of the genus Vitivirus have been identified recently, mainly due to the advent of high-throughput sequencing (HTS). Grapevine virus I (GVI), which was identified in New Zealand in 2018, is one of these viruses. RNAseq HTS analysis of a Greek grapevine (cv. Daphnia), revealed the presence of a GVI-like isolate (D2-1/19). Sequence analysis confirmed the classification of D2-1/19 as GVI. However, both sequence and phylogenetic data exhibited high levels of variability between D2-1/19 and the previously characterized GVI isolates. This study provides the full-length sequence of a divergent GVI isolate, adding knowledge to the limited information available about this recently identified virus.

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Authors and Affiliations

  1. Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloníki, Greece

    L. Lotos, P. Panailidou, N. I. Katis & V. I. Maliogka

  2. Instituto Valenciano de Investigaciones Agrarias, 46113, Moncada, Valencia, Spain

    A. B. Ruiz-García & A. Olmos

Authors
  1. L. Lotos
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  2. A. B. Ruiz-García
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  3. P. Panailidou
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  4. A. Olmos
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  5. N. I. Katis
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  6. V. I. Maliogka
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Correspondence to L. Lotos.

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Electronic supplementary material

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705_2020_4762_MOESM1_ESM.jpg

Supplementary material 1 Nucleotide sequence identity values for the replicase (ORF1), RdRp region, and 18 kDa (ORF2) coding regions of D2-1/19 and isolates of grapevine virus E, grapevine virus I, and grapevine virus L (JPEG 1249 kb)

705_2020_4762_MOESM2_ESM.jpg

Supplementary material 2 Nucleotide sequence identity value for the MP (ORF3), CP (ORF4), and NABP (ORF5) coding regions of D2-1/19 and isolates of grapevine virus E, grapevine virus I, and grapevine virus L (JPEG 1151 kb)

705_2020_4762_MOESM3_ESM.jpg

Supplementary material 3 Amino acid sequence identity values for the replicase (ORF1), RdRp region, and 18 kDa (ORF2) proteins of D2-1/19 and isolates of grapevine virus E, grapevine virus I, and grapevine virus L (JPEG 1233 kb)

705_2020_4762_MOESM4_ESM.jpg

Supplementary material 4 Amino acid sequence identity values for the MP (ORF3), CP (ORF4), and NABP (ORF5) proteins of D2-1/19 and isolates of grapevine virus E, grapevine virus I, and grapevine virus L (JPEG 1159 kb)

Supplementary material 5 (DOCX 15 kb)

Supplementary material 6 (DOCX 12 kb)

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Lotos, L., Ruiz-García, A.B., Panailidou, P. et al. The complete genome sequence of a divergent grapevine virus I isolate naturally infecting grapevine in Greece. Arch Virol 165, 3003–3006 (2020). https://doi.org/10.1007/s00705-020-04762-9

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  • DOI: https://doi.org/10.1007/s00705-020-04762-9

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