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Sorghum mastrevirus-associated alphasatellites: new geminialphasatellites associated with an African streak mastrevirus infecting wild Poaceae plants on Reunion Island

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Abstract

Nine complete nucleotide sequences of geminialphasatellites (subfamily Geminialphasatellitinae, family Alphasatellitidae) recovered from the wild Poaceae Sorghum arundinaceum collected in Reunion are described and analyzed. While the helper geminivirus was identified as an isolate of maize streak virus (genus Mastrevirus, family Geminiviridae), the geminialphasatellite genomes were most closely related to, and shared ~63% identity with, clecrusatellites. Even though the geminialphasatellite molecules lack an adenine rich-region, they have the typical size of geminialphasatellites, encode a replication-associated protein in the virion sense, and have probable stem-loop structures at their virion-strand origins of replication. According to the proposed geminialphasatellite species and genus demarcation thresholds (88% and 70% nucleotide identity, respectively), the genomes identified here represent a new species (within a new genus) for which we propose the name “Sorghum mastrevirus-associated alphasatellite” (genus “Sorgasalphasatellite”).

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References

  1. Rosario K, Padilla-Rodriguez M, Kraberger S, Stainton D, Martin DP, Breitbart M, Varsani A (2013) Discovery of a novel mastrevirus and alphasatellite-like circular DNA in dragonflies (Epiprocta) from Puerto Rico. Virus Res 171(1):231–237

    Article  CAS  Google Scholar 

  2. Kumar J, Kumar J, Singh SP, Tuli R (2014) Association of satellites with a mastrevirus in natural infection: complexity of wheat dwarf India virus disease. J Virol 88(12):7093–7104

    Article  Google Scholar 

  3. Hamza M, Tahir MN, Mustafa R, Kamal H, Khan MZ, Mansoor S, Briddon RW, Amin I (2018) Identification of a dicot infecting mastrevirus along with alpha-and betasatellite associated with leaf curl disease of spinach (Spinacia oleracea) in Pakistan. Virus Res 256:174–182

    Article  CAS  Google Scholar 

  4. Briddon RW, Martin DP, Roumagnac P, Navas-Castillo J, Fiallo-Olivé E, Moriones E, Lett JM, Zerbini FM, Varsani A (2018) Alphasatellitidae: A new family with two subfamilies for the classification of geminivirus-and nanovirus-associated alphasatellites. Arch Virol 163(9):2587–2600

    Article  CAS  Google Scholar 

  5. Lozano G, Trenado HP, Fiallo-Olive E, Chirinos D, Geraud-Pouey F, Briddon RW, Navas-Castillo J (2016) Characterization of NON-coding DNA Satellites associated with Sweepoviruses (Genus Begomovirus, Geminiviridae)—definition of a distinct class of Begomovirus-associated satellites. Front Microbiol 7:162

    PubMed  PubMed Central  Google Scholar 

  6. Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran S, Thierer T, Ashton B, Meintjes P, Drummond A (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647–1649

    Article  Google Scholar 

  7. Muhire BM, Varsani A, Martin DP (2014) SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PLoS One 9:9

    Google Scholar 

  8. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  Google Scholar 

  9. Koonin EV, Ilyina TV (1992) Geminivirus replication proteins are related to prokaryotic plasmid rolling circle DNA replication initiator proteins. J Gen Virol 73:2763

    Article  CAS  Google Scholar 

  10. Laufs J, Schumacher S, Geisler N, Jupin I, Gronenborn B (1995) Identification of the nicking tyrosine of geminivirus Rep protein. FEBS Lett 377:258–262

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was funded by the European Union (ERDF, contract GURDT I2016-1731-0006632), the Agropolis Fondation (Labex Agro – Montpellier, E-SPACE project number 1504-004), the Conseil Régional de la Réunion and CIRAD. SC is a recipient of a PhD fellowship from CIRAD and the Agropolis Fondation (E-SPACE). This work was conducted on the Plant Protection Platform (3P, IBISA).

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Authors and Affiliations

  1. CIRAD, UMR PVBMT, 97410, Saint-Pierre, La Réunion, France

    Sohini Claverie, Murielle Hoareau, Pierre Lefeuvre & Jean-Michel Lett

  2. Université de La Réunion, UMR PVBMT, 97410, Saint-Pierre, La Réunion, France

    Sohini Claverie

  3. The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, AZ, USA

    Arvind Varsani

  4. Structural Biology Research Unit, Department of Clinical Laboratory Sciences, University of Cape Town, Rondebosch, Cape Town, South Africa

    Arvind Varsani

  5. CIRAD, UMR BGPI, 34398, Montpellier, France

    Denis Filloux & Philippe Roumagnac

  6. BGPI, Université de Montpellier, CIRAD, INRA, Montpellier SupAgro, 34398, Montpellier, France

    Denis Filloux & Philippe Roumagnac

  7. Computational Biology Division, Department of Integrative Biomedical Sciences, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Observatory, South Africa

    Darren P. Martin

Authors
  1. Sohini Claverie
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  2. Arvind Varsani
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  3. Murielle Hoareau
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  4. Denis Filloux
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  5. Philippe Roumagnac
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  6. Darren P. Martin
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  7. Pierre Lefeuvre
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  8. Jean-Michel Lett
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Correspondence to Jean-Michel Lett.

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Claverie, S., Varsani, A., Hoareau, M. et al. Sorghum mastrevirus-associated alphasatellites: new geminialphasatellites associated with an African streak mastrevirus infecting wild Poaceae plants on Reunion Island. Arch Virol 165, 1925–1928 (2020). https://doi.org/10.1007/s00705-020-04685-5

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  • DOI: https://doi.org/10.1007/s00705-020-04685-5

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