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Genotype constellation of a rotavirus A field strain with an uncommon G8P[11] genotype combination in a rotavirus-vaccinated dairy cattle herd

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Abstract

In this report we describe the genotype constellation of a bovine rotavirus A (RVA) strain with an uncommon G8P[11] genotype combination. The RVA/Cow-wt/BRA/Y136/2017/G8P[11] strain was classified as G8-P[11]-I2-R5-C2-M2-A3-N2-T9-E2-H3. Phylogenetic analysis based on the VP7 gene showed that the Y136 strain and a human G8P[1] strain comprise a putative new (VII) lineage for the G8 genotype. In addition, two other genotypes, R5 (VP1) and T9 (NSP3), were identified in the constellation of Y136 that are rarely found in RVA strains of bovine origin. The immunological pressure caused by regular vaccination of cows might be responsible for the selection of heterologous RVA strains.

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References

  1. Abe M, Ito N, Masatani T, Nakagawa K, Yamaoka S, Kanamaru Y, Suzuki H, Shibano K, Arashi Y, Sugiyama M (2011) Whole genome characterization of new bovine rotavirus G21P[29] and G24P[33] strains provides evidence for interspecies transmission. J Gen Virol 92:952–960

    CAS  PubMed  Google Scholar 

  2. Agbemabiese CA, Nakagomi T, Doan YH, Nakagomi O (2015) Whole genomic constellation of the first human G8 rotavirus strain detected in Japan. Infect Genet Evol 35:184–193

    CAS  PubMed  Google Scholar 

  3. Alfieri AA, Parazzi ME, Takiuchi E, Medici KC, Alfieri AF (2006) Frequency of group A rotavirus in diarrhoeic calves in Brazilian cattle herds, 1998–2002. Trop Anim Health Prod 38:521–526

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Alfieri AF, Alfieri AA, Barreiros MA, Leite JP, Richtzenhain LJ (2004) G and P genotypes of group A rotavirus strains circulating in calves in Brazil, 1996–1999. Vet Microbiol 99:167–173

    PubMed  Google Scholar 

  5. Badaracco A, Garaicoechea L, Rodriguez D, Uriarte EL, Odeon A, Bilbao G, Galarza R, Abdala A, Fernandez F, Parreno V (2012) Bovine rotavirus strains circulating in beef and dairy herds in Argentina from 2004 to 2010. Vet Microbiol 158:394–399

    CAS  PubMed  Google Scholar 

  6. Banyai K, Kemenesi G, Budinski I, Foldes F, Zana B, Marton S, Varga-Kugler R, Oldal M, Kurucz K, Jakab F (2017) Candidate new rotavirus species in Schreiber’s bats, Serbia. Infect Genet Evol 48:19–26

    PubMed  Google Scholar 

  7. Barreiros MA, Alfieri AF, Medici KC, Leite JP, Alfieri AA (2004) G and P genotypes of group A rotavirus from diarrhoeic calves born to cows vaccinated against the NCDV (P[1], G6) rotavirus strain. J Vet Med 51:104–109

    CAS  Google Scholar 

  8. Browning GF, Snodgrass DR, Nakagomi O, Kaga E, Sarasini A, Gerna G (1992) Human and bovine serotype G8 rotaviruses may be derived by reassortment. Arch Virol 125:121–128

    CAS  PubMed  Google Scholar 

  9. Cashman O, Lennon G, Sleator RD, Power E, Fanning S, O’Shea H (2010) Changing profile of the bovine rotavirus G6 population in the south of Ireland from 2002 to 2009. Vet Microbiol 146:238–244

    CAS  PubMed  Google Scholar 

  10. Doan YH, Nakagomi T, Nakagomi O (2012) Repeated circulation over 6 years of intergenogroup mono-reassortant G2P[4] rotavirus strains with genotype N1 of the NSP2 gene. Infect Genet Evol 12:1202–1212

    PubMed  Google Scholar 

  11. Dulgheroff AC, Silva GA, Naveca FG, Oliveira AG, Domingues AL (2016) Diversity of group A rotavirus genes detected in the Triangulo Mineiro region, Minas Gerais, Brazil. Braz J Microbiol 47:731–740

    PubMed  PubMed Central  Google Scholar 

  12. Falcone E, Tarantino M, Di Trani L, Cordioli P, Lavazza A, Tollis M (1999) Determination of bovine rotavirus G and P serotypes in italy by PCR. J Clin Microbiol 37:3879–3882

    CAS  PubMed  PubMed Central  Google Scholar 

  13. Fritzen JTT, Lorenzetti E, Oliveira MV, Bon VR, Ayres H, Alfieri AF, Alfieri AA (2019) Cross-sectional study of the G and P genotypes of rotavirus A field strains circulating in regularly vaccinated dairy cattle herds. Trop Anim Health Prod 51:887–892

    PubMed  Google Scholar 

  14. Fritzen JTT, Oliveira MV, Lorenzetti E, Miyabe FM, Viziack MP, Rodrigues CA, Ayres H, Alfieri AF, Alfieri AA (2019) Longitudinal surveillance of rotavirus A genotypes circulating in a high milk yield dairy cattle herd after the introduction of a rotavirus vaccine. Vet Microbiol 230:260–264

    PubMed  PubMed Central  Google Scholar 

  15. Fukai K, Sakai T, Hirose M, Itou T (1999) Prevalence of calf diarrhea caused by bovine group A rotavirus carrying G serotype 8 specificity. Vet Microbiol 66:301–311

    CAS  PubMed  Google Scholar 

  16. Fukai K, Onoda H, Itou T, Sato M, Miura Y, Sakai T (2004) Genetic and serological characterization of novel serotype G8 bovine group A rotavirus strains isolated in Japan. J Vet Med Sci 66:1413–1416

    PubMed  Google Scholar 

  17. Gomez MM, Volotao EM, de Mendonca MC, Tort LF, da Silva MF, Leite JP (2010) Detection of uncommon rotavirus A strains P[8]G8 and P[4]G8 in the city of Rio de Janeiro, 2002. J Med Virol 82:1272–1276

    CAS  PubMed  Google Scholar 

  18. Gouvea V, Glass RI, Woods P, Taniguchi K, Clark HF, Forrester B, Fang ZY (1990) Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol 28:276–282

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Hoshino Y, Honma S, Jones RW, Santos N, Nakagomi O, Nakagomi T, Kapikian AZ, Thouless ME (2006) A rotavirus strain isolated from pig-tailed macaque (Macaca nemestrina) with diarrhea bears a P6[1]:G8 specificity. Virology 345:1–12

    CAS  PubMed  Google Scholar 

  20. ICTV (2020) International Committee on Taxonomy of Viruses

  21. Jere KC, Esona MD, Ali YH, Peenze I, Roy S, Bowen MD, Saeed IK, Khalafalla AI, Nyaga MM, Mphahlele J, Steele D, Seheri ML (2014) Novel NSP1 genotype characterised in an African camel G8P[11] rotavirus strain. Infect Genet Evol 21:58–66

    CAS  PubMed  Google Scholar 

  22. Karayel I, Feher E, Marton S, Coskun N, Banyai K, Alkan F (2017) Putative vaccine breakthrough event associated with heterotypic rotavirus infection in newborn calves, Turkey, 2015. Vet Microbiol 201:7–13

    CAS  PubMed  PubMed Central  Google Scholar 

  23. Komoto S, Pongsuwanna Y, Tacharoenmuang R, Guntapong R, Ide T, Higo-Moriguchi K, Tsuji T, Yoshikawa T, Taniguchi K (2016) Whole genomic analysis of bovine group A rotavirus strains A5-10 and A5-13 provides evidence for close evolutionary relationship with human rotaviruses. Vet Microbiol 195:37–57

    CAS  PubMed  Google Scholar 

  24. Louge Uriarte EL, Badaracco A, Matthijnssens J, Zeller M, Heylen E, Manazza J, Mino S, Van Ranst M, Odeon A, Parreno V (2014) The first caprine rotavirus detected in Argentina displays genomic features resembling virus strains infecting members of the Bovidae and Camelidae. Vet Microbiol 171:189–197

    CAS  PubMed  Google Scholar 

  25. Luchs A, Timenetsky Mdo C (2014) G8P[6] rotaviruses isolated from Amerindian children in Mato Grosso do Sul, Brazil, during 2009: close relationship of the G and P genes with those of bovine and bat strains. J Gen Virol 95:627–641

    CAS  PubMed  Google Scholar 

  26. Martella V, Ciarlet M, Banyai K, Lorusso E, Cavalli A, Corrente M, Elia G, Arista S, Camero M, Desario C, Decaro N, Lavazza A, Buonavoglia C (2006) Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain. Virology 346:301–311

    CAS  PubMed  Google Scholar 

  27. Martinez M, Phan TG, Galeano ME, Russomando G, Parreno V, Delwart E, Parra GI (2014) Genomic characterization of a rotavirus G8P[1] detected in a child with diarrhea reveal direct animal-to-human transmission. Infect Genet Evol 27:402–407

    CAS  PubMed  Google Scholar 

  28. Matthijnssens J, Ciarlet M, Heiman E, Arijs I, Delbeke T, McDonald SM, Palombo EA, Iturriza-Gomara M, Maes P, Patton JT, Rahman M, Van Ranst M (2008) Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains. J Virol 82:3204–3219

    CAS  PubMed  PubMed Central  Google Scholar 

  29. Matthijnssens J, Ciarlet M, Rahman M, Attoui H, Banyai K, Estes MK, Gentsch JR, Iturriza-Gomara M, Kirkwood CD, Martella V, Mertens PP, Nakagomi O, Patton JT, Ruggeri FM, Saif LJ, Santos N, Steyer A, Taniguchi K, Desselberger U, Van Ranst M (2008) Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments. Arch Virol 153:1621–1629

    CAS  PubMed  PubMed Central  Google Scholar 

  30. Matthijnssens J, Bilcke J, Ciarlet M, Martella V, Banyai K, Rahman M, Zeller M, Beutels P, Van Damme P, Van Ranst M (2009) Rotavirus disease and vaccination: impact on genotype diversity. Future Microbiol 4:1303–1316

    CAS  PubMed  Google Scholar 

  31. Matthijnssens J, Potgieter CA, Ciarlet M, Parreno V, Martella V, Banyai K, Garaicoechea L, Palombo EA, Novo L, Zeller M, Arista S, Gerna G, Rahman M, Van Ranst M (2009) Are human P[14] rotavirus strains the result of interspecies transmissions from sheep or other ungulates that belong to the mammalian order Artiodactyla? J Virol 83:2917–2929

    CAS  PubMed  PubMed Central  Google Scholar 

  32. Matthijnssens J, Ciarlet M, McDonald SM, Attoui H, Banyai K, Brister JR, Buesa J, Esona MD, Estes MK, Gentsch JR, Iturriza-Gomara M, Johne R, Kirkwood CD, Martella V, Mertens PP, Nakagomi O, Parreno V, Rahman M, Ruggeri FM, Saif LJ, Santos N, Steyer A, Taniguchi K, Patton JT, Desselberger U, Van Ranst M (2011) Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG). Arch Virol 156:1397–1413

    CAS  PubMed  PubMed Central  Google Scholar 

  33. Medeiros TNS, Lorenzetti E, Alfieri AF, Alfieri AA (2015) Phylogenetic analysis of a G6P[5] bovine rotavirus strain isolated in a neonatal diarrhea outbreak in a beef cattle herd vaccinated with G6P[1] and G10P[11] genotypes. Arch Virol 160:447–451

    Google Scholar 

  34. Medeiros TNS, Lorenzetti E, Alfieri AF, Alfieri AA (2019) G and P genotype profiles of rotavirus A field strains circulating in beef and dairy cattle herds in Brazil, 2006–2015. Compar Immunol Microbiol Infect Dis 64:90–98

    Google Scholar 

  35. Medici MC, Abelli LA, Martinelli M, Dettori G, Chezzi C (2008) Molecular characterization of VP4, VP6 and VP7 genes of a rare G8P[14] rotavirus strain detected in an infant with gastroenteritis in Italy. Virus Res 137:163–167

    CAS  PubMed  Google Scholar 

  36. Montenegro FM, Correia JB, Rodrigues Falbo A, Dove W, Nakagomi T, Nakagomi O, Cuevas LE, Cunliffe NA, Hart CA (2007) Anticipating rotavirus vaccines in Brazil: detection and molecular characterization of emerging rotavirus serotypes G8 and G9 among children with diarrhoea in Recife, Brazil. J Med Virol 79:335–340

    PubMed  Google Scholar 

  37. Nakagomi T, Chang BR, Nakagomi O (2009) Rotavirus hospitalization and molecular epidemiology in northern Japan, 1987–1996. Vaccine 27(Suppl 5):F93–96

    PubMed  Google Scholar 

  38. Okada N, Matsumoto Y (2002) Bovine rotavirus G and P types and sequence analysis of the VP7 gene of two G8 bovine rotaviruses from Japan. Vet Microbiol 84:297–305

    CAS  PubMed  Google Scholar 

  39. Okitsu S, Hikita T, Thongprachum A, Khamrin P, Takanashi S, Hayakawa S, Maneekarn N, Ushijima H (2018) Detection and molecular characterization of two rare G8P[14] and G3P[3] rotavirus strains collected from children with acute gastroenteritis in Japan. Infect Genet Evol 62:95–108

    CAS  PubMed  Google Scholar 

  40. Papp H, Laszlo B, Jakab F, Ganesh B, De Grazia S, Matthijnssens J, Ciarlet M, Martella V, Banyai K (2013) Review of group A rotavirus strains reported in swine and cattle. Vet Microbiol 165:190–199

    CAS  PubMed  PubMed Central  Google Scholar 

  41. Rocha TG, Silva FD, Gregori F, Alfieri AA, Buzinaro MD, Fagliari JJ (2017) Longitudinal study of bovine rotavirus group A in newborn calves from vaccinated and unvaccinated dairy herds. Trop Anim Health Prod 49:783–790

    PubMed  PubMed Central  Google Scholar 

  42. Rosa ESML, Pires De Carvalho I, Gouvea V (2002) 1998-1999 rotavirus seasons in Juiz de Fora, Minas Gerais, Brazil: detection of an unusual G3P[4] epidemic strain. J Clin Microbiol 40:2837–2842

    Google Scholar 

  43. Santos N, Lima RC, Pereira CF, Gouvea V (1998) Detection of rotavirus types G8 and G10 among Brazilian children with diarrhea. J Clin Microbiol 36:2727–2729

    CAS  PubMed  PubMed Central  Google Scholar 

  44. Santos VS, Nobrega FA, Soares MWS, Moreira RD, Cuevas LE, Gurgel RQ (2018) Rotavirus genotypes circulating in Brazil before and after the national rotavirus vaccine program: a review. Pediatr Infect Dis J 37:e63–e65

    PubMed  Google Scholar 

  45. Sharma S, Paul VK, Bhan MK, Ray P (2009) Genomic characterization of nontypeable rotaviruses and detection of a rare G8 strain in Delhi, India. J Clin Microbiol 47:3998–4005

    CAS  PubMed  PubMed Central  Google Scholar 

  46. Taniguchi K, Urasawa T, Pongsuwanna Y, Choonthanom M, Jayavasu C, Urasawa S (1991) Molecular and antigenic analyses of serotypes 8 and 10 of bovine rotaviruses in Thailand. J Gen Virol 72(Pt 12):2929–2937

    CAS  PubMed  Google Scholar 

  47. Taniguchi K, Urasawa T, Urasawa S (1993) Independent segregation of the VP4 and the VP7 genes in bovine rotaviruses as confirmed by VP4 sequence analysis of G8 and G10 bovine rotavirus strains. J Gen Virol 74(Pt 6):1215–1221

    CAS  PubMed  Google Scholar 

  48. Usonis V, Ivaskeviciene I, Desselberger U, Rodrigo C, Pediatric REC (2012) The unpredictable diversity of co-circulating rotavirus types in Europe and the possible impact of universal mass vaccination programmes on rotavirus genotype incidence. Vaccine 30:4596–4605

    PubMed  Google Scholar 

  49. Volotao EM, Soares CC, Maranhao AG, Rocha LN, Hoshino Y, Santos N (2006) Rotavirus surveillance in the city of Rio de Janeiro-Brazil during 2000–2004: detection of unusual strains with G8P[4] or G10P[9] specificities. J Med Virol 78:263–272

    CAS  PubMed  Google Scholar 

  50. Ward ML, Mijatovic-Rustempasic S, Roy S, Rungsrisuriyachai K, Boom JA, Sahni LC, Baker CJ, Rench MA, Wikswo ME, Payne DC, Parashar UD, Bowen MD (2016) Molecular characterization of the first G24P[14] rotavirus strain detected in humans. Infect Genet Evol 43:338–342

    CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

The authors thank the following Brazilian Institutes for financial support: National Council for Scientific and Technological Development (CNPq), the Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES), Araucária Foundation (FAP/PR), and National Institute of Science and Technology for Dairy Production Chain (INCT–LEITE, grant number 465725/2014–7). Alfieri, A.A. and Alfieri, A.F. are recipients of CNPq fellowships.

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Authors and Affiliations

  1. Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid-Campus Universitário, PO Box 10011, Londrina, Paraná, 86057-970, Brazil

    Juliana T. T. Fritzen, Marcos V. Oliveira, Elis Lorenzetti, Alice F. Alfieri & Amauri A. Alfieri

  2. Post Graduate Program in Animal Health and Production, Universidade Pitágoras Unopar, Arapongas, Paraná, Brazil

    Elis Lorenzetti

  3. Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Brazil

    Elis Lorenzetti & Alice F. Alfieri

  4. National Institute of Science and Technology for Dairy Production Chain (INCT–LEITE), Universidade Estadual de Londrina, Londrina, Brazil

    Amauri A. Alfieri

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  1. Juliana T. T. Fritzen
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Correspondence to Amauri A. Alfieri.

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Fritzen, J.T.T., Oliveira, M.V., Lorenzetti, E. et al. Genotype constellation of a rotavirus A field strain with an uncommon G8P[11] genotype combination in a rotavirus-vaccinated dairy cattle herd. Arch Virol 165, 1855–1861 (2020). https://doi.org/10.1007/s00705-020-04675-7

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