Skip to main content

Advertisement

SpringerLink
Log in

Identification and genetic characterization of a porcine hepe-astrovirus (bastrovirus) in the United States

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Here we describe the identification and genetic characterization of a porcine hepe-astrovirus, or bastrovirus, obtained from feces from pigs in the United States. The genome of the new bastrovirus is 5,955 nt long and contains two open reading frames (ORFs). ORF1 encodes a protein containing three domains, viral methyltransferase, RNA helicase and RNA-dependent RNA polymerase (RdRp), and is closely related to the RdRp of hepatitis E virus. The ORF2 protein shares similarities with the astrovirus capsid precursor protein. Although structural features of bastroviruses may resemble those of astroviruses, distinct characteristics of the newly identified bastrovirus include the presence of an RNA helicase domain in ORF1 and the lack of ORF1b. In addition to genetic characterization, screening of 368 porcine samples (oral fluids, oral swabs or fecal swabs) collected in the United States (US) using a porcine-bastrovirus-specific real-time PCR assay revealed that 31% of those samples were positive. These results suggest a broad distribution of bastroviruses in the swine population in the US. This represents the first description of bastrovirus in swine in the US.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Cortez V, Meliopoulos VA, Karlsson EA et al (2017) Astrovirus Biology and Pathogenesis. Annu Rev Virol. https://doi.org/10.1146/annurev-virology-101416-041742

    Article  PubMed  Google Scholar 

  2. Shimizu M, Shirai J, Narita M, Yamane T (1990) Cytopathic astrovirus isolated from porcine acute gastroenteritis in an established cell line derived from porcine embryonic kidney. J Clin Microbiol 28:201–206

    CAS  PubMed  PubMed Central  Google Scholar 

  3. ICTV (2018) Virus taxonomy: 2018 release. ICTV 1:18

    Google Scholar 

  4. Xiao CT, Giménez-Lirola LG, Gerber PF et al (2013) Identification and characterization of novel porcine astroviruses (PAstVs) with high prevalence and frequent co-infection of individual pigs with multiple PAstV types. J Gen Virol. https://doi.org/10.1099/vir.0.048744-0

    Article  PubMed  PubMed Central  Google Scholar 

  5. Padmanabhan A, Hause BM (2016) Detection and characterization of a novel genotype of porcine astrovirus 4 from nasal swabs from pigs with acute respiratory disease. Arch Virol. https://doi.org/10.1007/s00705-016-2937-1

    Article  PubMed  Google Scholar 

  6. Boros Á, Albert M, Pankovics P et al (2017) Outbreaks of neuroinvasive astrovirus associated with encephalomyelitis, weakness, and paralysis among weaned pigs. Emerg Infect Dis, Hungary. https://doi.org/10.3201/eid2312.170804

    Book  Google Scholar 

  7. Arruda B, Arruda P, Hensch M et al (2017) Porcine astrovirus type 3 in central nervous system of swine with polioencephalomyelitis. Emerg Infect Dis 23:2097. https://doi.org/10.3201/eid2312.170703

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Blomström A-L, Ley C, Jacobson M (2014) Astrovirus as a possible cause of congenital tremor type AII in piglets? Acta Vet Scand 56:82. https://doi.org/10.1186/s13028-014-0082-y

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Jiang B, Monroe SS, Koonin EV et al (1993) RNA sequence of astrovirus: distinctive genomic organization and a putative retrovirus-like ribosomal frameshifting signal that directs the viral replicase synthesis. Proc Natl Acad Sci 90:10539–10543. https://doi.org/10.1073/pnas.90.22.10539

    Article  CAS  PubMed  Google Scholar 

  10. Mendez E, Arias, CF. Astroviridae. (2013) In: Knipe DM, Howley PM (eds.) Fields Virology. Chapter 21. 6th ed. Lippincott Williams and Wilkins. p 609

  11. Oude Munnink BB, Cotten M, Canuti M et al (2016) A novel astrovirus-like RNA virus detected in human stool. Virus Evol 2:1. https://doi.org/10.1093/ve/vew005

    Article  Google Scholar 

  12. Yinda CK, Ghogomu SM, Conceição-Neto N et al (2018) Cameroonian fruit bats harbor divergent viruses, including rotavirus H, bastroviruses, and picobirnaviruses using an alternative genetic code. Virus Evol 4:1. https://doi.org/10.1093/ve/vey008

    Article  Google Scholar 

  13. Reuter G, Boros Á, Tóth Z et al (2018) Detection of a novel RNA virus with hepatitis E virus-like non-structural genome organization in amphibian, agile frog (Rana dalmatina) tadpoles. Infect Genet Evol 65:112–116. https://doi.org/10.1016/j.meegid.2018.07.029

    Article  CAS  PubMed  Google Scholar 

  14. Hause BM, Collin EA, Anderson J et al (2015) Bovine rhinitis viruses are common in US cattle with bovine respiratory disease. PLoS One 10:e121998. https://doi.org/10.1371/journal.pone.0121998

    Article  CAS  Google Scholar 

  15. Meier-Stephenson V, Mrozowich T, Pham M, Patel TR (2018) DEAD-box helicases: the Yin and Yang roles in viral infections. Biotechnol Genet Eng Rev 34:3–32. https://doi.org/10.1080/02648725.2018.1467146

    Article  CAS  PubMed  Google Scholar 

  16. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  Google Scholar 

  17. Kumar S, Stecher G, Tamura K, Dudley J (2016) MEGA7: molecular evolutionary genetics analysis version 70 for bigger datasets downloaded from. Mol Biol Evol 30:2725–2729. https://doi.org/10.1093/molbev/msw054

    Article  CAS  Google Scholar 

Download references

Funding

This study was funded by the USDA National Institute of Food and Agriculture (Grants SD00H517-14 and SD00R518-14).

Author information

Authors and Affiliations

  1. Animal Disease Research and Diagnostic Laboratory (ADRDL), Department of Veterinary and Biomedical Sciences, South Dakota State University, Box 2175, 1155 N Campus Drive, SAR118, Brookings, SD, 57007, USA

    Fernando V. Bauermann, Alaine R. Buysse, Lok R. Joshi & Diego G. Diel

  2. Department of Veterinary Pathobiology, Oklahoma State University, 250 McElroy Hall, Stillwater, OK, 74074, USA

    Fernando V. Bauermann

  3. Cambridge Technologies, 1525 Bioscience Drive, Worthington, MN, 56187, USA

    Ben Hause

Authors
  1. Fernando V. Bauermann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ben Hause
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alaine R. Buysse
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lok R. Joshi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Diego G. Diel
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Diego G. Diel.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Handling Editor: Andrew Edwin Firth.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bauermann, F.V., Hause, B., Buysse, A.R. et al. Identification and genetic characterization of a porcine hepe-astrovirus (bastrovirus) in the United States. Arch Virol 164, 2321–2326 (2019). https://doi.org/10.1007/s00705-019-04313-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-019-04313-x

Search

Navigation