Abstract
By screening a collection of Fusarium spp. for the presence of dsRNA, the Fusarium redolens strain A63-1 was found harboring a pattern of multiple dsRNA bands when analyzed by agarose gel electrophoresis. Using NextSeq Illumina sequencing, the full sequences of eight dsRNA molecules were determined, compared to databases, and gathered into a new viral genome. This novel virus shares similarities with mycoviruses that were recently grouped in the proposed family “Polymycoviridae”. Hence, the name “Fusarium redolens polymycovirus 1” is proposed for this virus. Each viral dsRNA contains only one ORF, except dsRNA 7, which has an additional one. Based on amino acid sequence similarities, the functions of the proteins encoded by dsRNA 1–4 can be hypothesized. On the other hand, the putative proteins encoded by dsRNA 5–8 exhibit no relevant homology to known proteins. In this report, the full genome sequence of this new virus is presented along with a primary bioinformatics analysis.
Similar content being viewed by others
References
Hollings M (1962) Viruses associated with a die-back disease of cultivated mushroom. Nature 196:962–965
Suzuki N (2017) Frontiers in fungal virology. J Gen Plant Pathol 83(6):419–423
Ghabrial S, Castón J, Jiang D, Nibert M, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479–480:356–368
Xie J, Jiang D (2014) New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Ann Rev Phytopathol 52:45–68
Sharma M, Guleria S, Singh K, Chauhan A, Kulshrestha S (2018) Mycovirus associated hypovirulence, a potential method for biological control of Fusarium species. Virus Dis 29(2):134–140
Aoki T, O’Donnell K, Geiser DM (2014) Systematics of key phytopathogenic Fusarium species: current status and future challenges. J Gen Plant Pathol 80(3):189–201
Douglas AP, Chen SC, Slavin MA (2016) Emerging infections caused by non-Aspergillus filamentous fungi. Clin Microbiol Infect 22(8):670–680
Cho W, Lee K, Yu J, Son M, Kim K (2013) Insight into mycoviruses infecting Fusarium species. Adv Virus Res 86:273–288
Scauflaire J, Mahieu O, Louvieaux J, Foucart G, Renard F, Munaut F (2011) Biodiversity of Fusarium species in ears and stalks of maize plants in Belgium. Eur J Plant Pathol 131:59–66
Leslie JF, Summerell BA (2006) The Fusarium laboratory manual. Wiley, Amsterdam
Khankhum S, Escalante C, Rodrigues de Souto E, Valverde R (2016) Extraction and electrophoretic analysis of large dsRNAs from desiccated plant tissues infected with plant viruses and biotrophic fungi. Eur J Plant Pathol 147(2):431–441
Afgan E, Baker D, Batut B, van den Beek M, Bouvier D, Cech M, Chilton J, Clements D, Coraor N, Grüning B, Guerler A, Hillman-Jackson J, Hiltemann S, Jalili V, Rasche H, Soranzo N, Goecks J, Taylor J, Nekrutenko A, Blankenberg D (2018) The Galaxy platform for accessible, reproductible and collaborative biomedical analyses: 2018 update. Nucl Acids Res 46(W1):537–544
Bolger AM, Lohse M, Usadel B (2014) Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 15(15):2114–2120
Kim D, Langmead B, Salzberg SL (2015) HISAT: a fast spliced aligner with low memory requirements. Nat Methods 12(4):357–360
Grabherr M, Haas B, Yassour M, Levin J, Thompson D, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng Q, Chen Z, Mauceli E, Birren BW, Nusbaum C, Lindblad-Toh K, Friedman N, Regev A, Hacohen N, Gnirke A, Rhind N, di Palma F (2011) Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol 29(7):644–652
Cock P, Chilton J, Grüning B, Johnson J, Soranzo N (2015) NCBI BLAST+ integrated into Galaxy. Gigascience 4:39
Okonechnikov K, Golosova O, Fursov M, U. team (2012) Unipro UGENE: a unified bioinformatics toolkit. Bioinformatics 28(8):1166–1167
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evolut 35:1547–1549
Jia H, Dong K, Zhou L, Wang G, Hong N, Jiang D, Xu W (2016) A dsRNA virus with filamentous viral particles. Nat Commun 8(1):168
Kotta-Loizou I, Coutts RHA (2017) Studies on the virome of the entomopathogenic fungus Beauveria bassiana reveal novel dsRNA elements and mild hypervirulence. PLOS Pathogens 13(1):e1006183
Kanhayuwa L, Kotta-Loizou I, Özkan S, Gunning AP, Coutts RHA (2015) A novel mycovirus from Aspergillus fumigatus contains four unique dsRNAs as its genome and is infectious as dsRNA. Proc Natl Acad Sci 112(29):9100–9105
Zhai L, Xiang J, Zhang M, Fu M, Yang Z, Hong N, Wang G (2016) Characterization of a novel double-stranded RNA mycovirus conferring hypovirulence from the phytopathogenic fungus Botryosphaeria dothidea. Virology 493:75–85
Author information
Authors and Affiliations
Corresponding author
Additional information
Handling Editor: Robert H.A. Coutts.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Mahillon, M., Decroës, A., Liénard, C. et al. Full genome sequence of a new polymycovirus infecting Fusarium redolens. Arch Virol 164, 2215–2219 (2019). https://doi.org/10.1007/s00705-019-04301-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-019-04301-1