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Role of conserved cysteine residues in the CAIC motif of the SU glycoprotein in the maturation and fusion activity of bovine leukaemia virus

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Abstract

The surface (SU) and transmembrane (TM) glycoproteins of many retroviruses are linked by disulphide bonds, and the interaction of SU with a cellular receptor results in disulphide bond isomerisation triggered by the CXXC motif in SU. This reaction leads to the fusion of viral and host cell membranes. In this work, we show that the cysteine at amino acid position 212 in the CAIC motif of the SU glycoprotein of bovine leukaemia virus has a free thiol group. A C-to-A mutation at position 212, either individually or in combination with a C-to-A mutation at position 215, was found to inhibit the maturation process, suggesting its involvement in the formation of the covalent bond with TM.

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Acknowledgements

This research did not receive any specific grants from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, Perugia, Italy

    Anna Serroni, Katia Forti & Antonio De Giuseppe

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  1. Anna Serroni
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  2. Katia Forti
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  3. Antonio De Giuseppe
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Correspondence to Katia Forti.

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Serroni, A., Forti, K. & De Giuseppe, A. Role of conserved cysteine residues in the CAIC motif of the SU glycoprotein in the maturation and fusion activity of bovine leukaemia virus. Arch Virol 164, 2309–2314 (2019). https://doi.org/10.1007/s00705-019-04294-x

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  • DOI: https://doi.org/10.1007/s00705-019-04294-x

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