Skip to main content

Advertisement

SpringerLink
Log in

Molecular analysis and serological survey of Tembusu virus infection in Zhejiang, China, 2010-2016

  • Original Article
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Tembusu virus (TMUV) is an important pathogen that causes acute egg drop syndrome in poultry. To investigate the epidemiological status of Tembusu virus in Zhejiang province, we collected clinical samples and sera from the local area from 2010 to 2016. A total of 41 out of the 88 collected tissue samples were identified as TMUV-infected by RT-PCR and were confirmed by sequencing. Six TMUV strains were isolated from TMUV-positive samples, and their complete genome sequences were determined. In addition, 19 E gene sequences amplified from RT-PCR-positive samples were determined. Sequence identity values among the 19 E genes and reference E sequences ranged from 96.8% to 100.0%, and they ranged from 97.3% to 99.9% when comparing the six genome sequences and references. Nineteen sites with amino acid mutations were identified in the E protein of nineteen strains, and these were at positions that are usually conserved in other TMUV strains. Antibodies to TMUV in serum samples were detected by indirect ELISA using recombinant EDIII (domain III of the E protein) as the antigen. The results showed that TMUV-specific antibodies were widely present in duck populations, with positive rates of 17.38%, 21.99%, 26.68%, and 17.79% in 2013, 2014, 2015 and 2016, respectively. The data from this study provide a good understanding of the epidemiology of TMUV infections in Zhejiang, China.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Cao Z et al (2011) Tembusu virus in ducks, china. Emerg Infect Dis 17(10):1873–1875

    Article  Google Scholar 

  2. Yan P et al (2011) An infectious disease of ducks caused by a newly emerged Tembusu virus strain in mainland China. Virology 417(1):1–8

    Article  CAS  Google Scholar 

  3. Su J et al (2011) Duck egg-drop syndrome caused by BYD virus, a new Tembusu-related flavivirus. PLos One 6(3):e18106

    Article  CAS  Google Scholar 

  4. Homonnay ZG et al (2014) Tembusu-like flavivirus (Perak virus) as the cause of neurological disease outbreaks in young Pekin duck. Avian Pathol 43(6):552–560

    Article  Google Scholar 

  5. Platt GS, Al E (1975) Arbovirus infections in Sarawak, October 1968–February 1970. Tembusu and Sindbis virus isolations from mosquitoes. Ann Trop Med Parasitol 69(1):65–71

    Article  CAS  Google Scholar 

  6. Pandey BD et al (1999) Identification of a flavivirus isolated from mosquitos in Chiang Mai Thailand. Southeast Asian J Trop Med Public Health 30(1):161–165

    CAS  PubMed  Google Scholar 

  7. Kono Y et al (2000) Encephalitis and retarded growth of chicks caused by Sitiawan virus, a new isolate belonging to the genus Flavivirus. Am J Trop Med Hyg 63(1–2):94–101

    Article  CAS  Google Scholar 

  8. Lei W et al (2017) The genetic characteristics and evolution of Tembusu virus. Vet Microbiol 201:32–41

    Article  Google Scholar 

  9. Dai L, Li Z, Tao P (2015) Evolutionary analysis of Tembusu virus: evidence for the emergence of a dominant genotype. Infect Genet Evol 32:124–129

    Article  Google Scholar 

  10. Yu K et al (2013) Structural, antigenic, and evolutionary characterizations of the envelope protein of newly emerging Duck Tembusu Virus. PLos One 8(8):e71319

    Article  CAS  Google Scholar 

  11. Mackenzie JS, Gubler DJ, Petersen LR (2004) Emerging flaviviruses: the spread and resurgence of Japanese encephalitis, West Nile and dengue viruses. Nat Med 10(12):98–109

    Article  Google Scholar 

  12. Tang Y et al (2015) Isolation and genetic characterization of a Tembusu virus strain isolated from mosquitoes in Shandong, China. Transbound Emerg Dis 62(2):209–216

    Article  CAS  Google Scholar 

  13. Zhang Y et al (2015) Evidence of possible vertical transmission of Tembusu virus in ducks. Vet Microbiol 179(3–4):149–154

    Article  Google Scholar 

  14. Li X et al (2015) Airborne transmission of a novel Tembusu virus in ducks. J Clin Microbiol 53(8):2734–2736

    Article  CAS  Google Scholar 

  15. Huang X et al (2013) Identification and molecular characterization of a novel flavivirus isolated from geese in China. Res Vet Sci 94(3):774–780

    Article  CAS  Google Scholar 

  16. Guang-Hua FU et al. (2014) Genome sequence and phylogenetic analysis of Tembusu viruses isolated from chicken. Chin J Vet Sci 34(9):1418–1422

    Google Scholar 

  17. Liu P et al (2012) Genomic and antigenic characterization of the newly emerging Chinese duck egg-drop syndrome flavivirus: genomic comparison with Tembusu and Sitiawan viruses. J Gen Virol 93(10):2158–2170

    Article  CAS  Google Scholar 

  18. Tang Y et al (2013) Characterization of a Tembusu Virus isolated from naturally infected house sparrows (Passer domesticus) in Northern China. Transbound Emerg Dis 60(2):152–158

    Article  CAS  Google Scholar 

  19. Tang Y et al (2013) Tembusu virus in human, China. Transbound Emerg Dis 60(3):193–196

    Article  CAS  Google Scholar 

  20. Li C et al (2016) Epitope identification and application for diagnosis of duck Tembusu virus infections in ducks. Viruses 8(11):306

    Article  Google Scholar 

  21. Fritz R, Stiasny K, Heinz FX (2008) Identification of specific histidines as pH sensors in flavivirus membrane fusion. J Cell Biol 183(2):353–361

    Article  CAS  Google Scholar 

  22. Li C et al (2016) Identification of a new broadly cross-reactive epitope within domain III of the duck Tembusu virus E protein. Sci Rep 6:36288

    Article  CAS  Google Scholar 

  23. Davis CW et al (2006) The location of asparagine-linked glycans on West Nile virions controls their interactions with CD209 (dendritic cell-specific ICAM-3 grabbing nonintegrin). J Biol Chem 281(48):37183–37194

    Article  CAS  Google Scholar 

  24. Hanna SL et al (2005) N-linked glycosylation of west nile virus envelope proteins influences particle assembly and infectivity. J Virol 79(21):13262–13274

    Article  CAS  Google Scholar 

  25. Wang D et al (2016) Preparation and antigenicity analysis of monoclonal antibody against the truncated E protein of avian Tembusu virus. Chin Vet Sci 7:805–813 (in Chineses)

    Google Scholar 

Download references

Funding

This study was funded by National Key Technology R & D Program of China (Grant number 2015BAD12B01).

Author information

Author notes

  1. Xiaozhi Huang and Huibo Qiu contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, People’s Republic of China

    Xiaozhi Huang, Huibo Qiu, Xing Peng, Wenbin Zhao, Kaikun Mo, Yan Yan, Min Liao & Jiyong Zhou

  2. Yuyao Municipal Institute of Poultry Disease, Yuyao, 315400, People’s Republic of China

    Xinhao Lu

Authors
  1. Xiaozhi Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Huibo Qiu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xing Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wenbin Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xinhao Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kaikun Mo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yan Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Min Liao
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Jiyong Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Min Liao.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The animal treatments were approved and performed in accordance with the animal ethics guidelines and approved procedures of the Committee on the Ethics of Animal Care and Use of Zhejiang University (ZJU20170388).

Additional information

Handling Editor: Tatjana Avsic-Zupanc.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary data associated with this article can be found in Table S 1-5. (DOC 169 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Huang, X., Qiu, H., Peng, X. et al. Molecular analysis and serological survey of Tembusu virus infection in Zhejiang, China, 2010-2016. Arch Virol 163, 3225–3234 (2018). https://doi.org/10.1007/s00705-018-3994-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-018-3994-4

Search

Navigation