Abstract
To investigate canine kobuvirus (CaKoV) infection in southwest China, 107 fecal samples were collected from dogs with obvious diarrhea in Sichuan and Chongqing regions, China. CaKoV infection was detected in 54 diarrheic samples (50.46%) by RT-PCR targeting a partial fragment (504 bp) of the 3D gene. Comparison of these partial 3D gene sequences from 14 of these CaKoV-positive samples show 95.4%–99.0% nucleotide (nt) identity within this group, and nt identities ranging from 93.1% to 98.2% with previously reported CaKoV 3D gene sequences. Additionally, we amplified five VP1 gene sequences and analyzed the inferred phylogeny. Amino acid (aa) identities of the five VP1 gene sequences were 81.5%–89.4% with those previously reported. Furthermore, one complete CaKoV genome was successfully obtained from a positive sample and designated SMCD-59/CHN/2015. This genome consisted of 8,184 nt, and shared 92.9%–96.6% nt identity (97.6%–98.1% aa identity) with other reported CaKoV genomes. This study provides proof that CaKoV circulates in diarrheic dogs in southwest China, and that these viruses exhibit unique genetic characteristics.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Khamrin P, Maneekarn N, Okitsu S, Ushijima H (2014) Epidemiology of human and animal kobuviruses. Virusease 25(2):195–200
Kapoor A, Simmonds P, Dubovi EJ, Qaisar N, Henriquez JA, Medina J, Shields S, Lipkin WI (2011) Characterization of a canine homolog of human aichivirus. J Virol 85(21):11520–11525
Li L, Pesavento PA, Shan T, Leutenegger CM, Wang C, Delwart E (2011) Viruses in diarrhoeic dogs include novel kobuviruses and sapoviruses. J Gen Virol 92:2534–2541
Carmona-Vicente N, Buesa J, Brown PA, Merga JY, Darby AC, Stavisky J, Sadler L, Gaskell RM, Dawson S, Radford AD (2013) Phylogeny and prevalence of kobuviruses in dogs and cats in the UK. Vet Microbiol 164(3–4):246–252
Di Martino B, Di Felice E, Ceci C, Di Profio F, Marsilio F (2013) Canine kobuviruses in diarrhoeic dogs in Italy. Vet Microbiol 166(1–2):246–249
Oem JK, Choi JW, Lee MH, Lee KK, Choi KS (2014) Canine kobuvirus infections in Korean dogs. Arch Virol 159(10):2751–2755
Choi S, Lim SI, Kim YK, Cho YY, Song JY, An DJ (2014) Phylogenetic analysis of astrovirus and kobuvirus in Korean dogs. J Vet Med Sci 76(3):1141–1145
Olarte-Castillo XA, Heeger F, Mazzoni CJ, Greenwood AD, Fyumagwa R, Moehlman PD, Hofer H, East ML (2015) Molecular characterization of canine kobuvirus in wild carnivores and the domestic dog in Africa. Virology 477:89–97
Soma T, Matsubayashi M, Sasai K (2016) Detection of kobuvirus RNA in Japanese domestic dogs. J Vet Med Sci 78(11):1731–1735
Li C, Wei S, Guo D, Wang Z, Geng Y, Wang E, Zhao X, Su M, Wang X, Sun D (2015) Prevalence and phylogenetic analysis of canine kobuviruses in diarrhoetic dogs in northeast China. J Vet Med Sci 78(1):7–11
Kong N, Zuo Y, Wang Z, Yu H, Zhou EM, Shan T, Tong G (2016) Molecular characterization of new described kobuvirus in dogs with diarrhea in China. Springerplus 5(1):2047
Di Martino B, Di Profio F, Melegari I, Robetto S, Di Felice E, Orusa R, Marsilio F (2014) Molecular evidence of kobuviruses in free-ranging red foxes (Vulpes vulpes). Arch Virol 159(7):1803–1806
Ribeiro J, Headley SA, Diniz JA, Pereira AH, Lorenzetti E, Alfieri AA, Alfieri AF (2017) Extra-intestinal detection of canine kobuvirus in a puppy from Southern Brazil. Arch Virol 162(3):867–872
Reuter G, Boros Ákos, Pankovics P (2011) Kobuviruses—a comprehensive review. Rev Med Virol 21(1):32–41
Yamashita T, Ito M, Kabashima Y, Tsuzuki H, Fujiura A, Sakae K (2003) Isolation and characterization of a new species of kobuvirus associated with cattle. J Gen Virol 84:3069–3077
Chen L, Zhu L, Zhou YC, Xu ZW, Guo WZ, Yang WY (2013) Molecular and phylogenetic analysis of the porcine kobuvirus VP1 region using infected pigs from Sichuan Province, China. Virol J 10:281
Zhu L, Wang X, Ren J, Kotecha A, Walter TS, Yuan S, Yamashita T, Tuthill TJ, Fry EE, Rao Z, Stuart DI (2016) Structure of human Aichi virus and implications for receptor binding. Nat Microbiol 1(11):16150
Klima M, Chalupska D, Różycki B, Humpolickova J, Rezabkova L, Silhan J, Baumlova A, Dubankova A, Boura E (2017) Kobuviral non-structural 3A proteins act as molecular harnesses to hijack the host ACBD3 protein. Structure 25(2):219–230
Fujita K, Krishnakumar SS, Franco D, Paul AV, London E, Wimmer E (2007) Membrane topography of the hydrophobic anchor sequence of poliovirus 3a and 3ab proteins and the functional effect of 3a/3ab membrane association upon RNA replication. Biochemistry 46(17):5185–5199
Acknowledgements
This work was funded by the 13th Five-Year Plan National Science and Technology Support Program (Grant number 2016YFD0500907). And, we also thank Steven M. Thompson, from Liwen Bianji, Edanz Editing China (http://www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Handling Editor: Diego G. Diel.
Rights and permissions
About this article
Cite this article
Li, M., Yan, N., Wang, M. et al. Prevalence and genomic characteristics of canine kobuvirus in southwest China. Arch Virol 163, 459–466 (2018). https://doi.org/10.1007/s00705-017-3648-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-017-3648-y