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Complete genome sequence of two tomato-infecting begomoviruses in Venezuela: evidence of a putative novel species and a novel recombinant strain

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Abstract

At least six begomovirus species have been reported infecting tomato in Venezuela. In this study the complete genomes of two tomato-infecting begomovirus isolates (referred to as Trujillo-427 and Zulia-1084) were cloned and sequenced. Both isolates showed the typical genome organization of New World bipartite begomoviruses, with DNA-A genomic components displaying 88.8% and 90.3% similarity with established begomoviruses, for isolates Trujillo-427 and Zulia-1084, respectively. In accordance to the guidelines for begomovirus species demarcation, the Trujillo-427 isolate represents a putative new species and the name “Tomato wrinkled mosaic virus” is proposed. Meanwhile, Zulia-1084 represents a putative new strain classifiable within species Tomato chlorotic leaf distortion virus, for which a recombinant origin is suggested.

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Acknowledgements

This study was partly supported by Grant FONACIT-G-2000001610 of the Venezuelan Ministry of Higher Education, Science and Technology. G.R. is a Move-In Louvain postdoctoral fellow of the Université catholique de Louvain. A.G. holds a Chargé de Recherches fellowship from the National Fund for Scientific Research (FNRS).

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Authors and Affiliations

  1. Earth and Life Institute, Applied Microbiology, Phytopathology, Université catholique de Louvain, Croix du Sud 2-L07.05.03, 1348, Louvain-la-Neuve, Belgium

    Gustavo Romay & Claude Bragard

  2. Unidad Técnica Fitosanitaria, La Universidad del Zulia (LUZ), Maracaibo, 4005, Estado Zulia, Venezuela

    Dorys T. Chirinos & Francis Geraud-Pouey

  3. Laboratorio de Entomología, Facultad de Ciencias Agrarias, Universidad Agraria del Ecuador, Guayaquil, Ecuador

    Dorys T. Chirinos

  4. Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Applied Microbiology, Université catholique de Louvain, Croix du Sud 2-L7.05.12, 1348, Louvain-la-Neuve, Belgium

    Annika Gillis & Jacques Mahillon

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  1. Gustavo Romay
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  2. Dorys T. Chirinos
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  3. Francis Geraud-Pouey
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  4. Annika Gillis
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  5. Jacques Mahillon
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  6. Claude Bragard
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Correspondence to Claude Bragard.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: F. Murilo Zerbini.

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Romay, G., Chirinos, D.T., Geraud-Pouey, F. et al. Complete genome sequence of two tomato-infecting begomoviruses in Venezuela: evidence of a putative novel species and a novel recombinant strain. Arch Virol 163, 555–558 (2018). https://doi.org/10.1007/s00705-017-3611-y

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  • DOI: https://doi.org/10.1007/s00705-017-3611-y

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