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Molecular analysis of the low-temperature Escherichia coli phage vB_EcoS_NBD2

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Abstract

A novel low-temperature Escherichia coli phage vB_EcoS_NBD2 was isolated in Lithuania from agricultural soil. With an optimum temperature for plating around 20 °C, vB_EcoS_NBD2 efficiently produced plaques on Escherichia coli NovaBlue (DE3) at a temperature range of 10–30 °C, yet failed to plate at temperatures above 35 °C. Phage vB_EcoS_NBD2 virions have a siphoviral morphology with an isometric head (65 nm in diameter), and a non-contractile flexible tail (170 nm). The 51,802-bp genome of vB_EcoS_NBD2 has a G + C content of 49.8%, and contains 87 probable protein-encoding genes as well as 1 gene for tRNASer. Comparative sequence analysis revealed that 22 vB_EcoS_NBD2 ORFs encode unique proteins that have no reliable identity to database entries. Based on homology to biologically defined proteins and/or proteomics analysis, 36 vB_EcoS_NBD2 ORFs were given a putative functional annotation, including 20 genes coding for morphogenesis-related proteins and 13 associated with DNA metabolism. Phylogenetic analysis revealed that vB_EcoS_NBD2 belongs to the subfamily Tunavirinae, but cannot be assigned to any genus currently recognized by ICTV.

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Acknowledgments

This work was supported by the Research Council of Lithuania (Project No. SIT-7/2015).

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Author notes

  1. Laura Kaliniene, Lidija Truncaitė and Eugenijus Šimoliūnas contributed equally to this work.

Authors and Affiliations

  1. Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio al. 7, 10257, Vilnius, Lithuania

    Laura Kaliniene, Lidija Truncaitė, Eugenijus Šimoliūnas, Aurelija Zajančkauskaitė, Monika Vilkaitytė & Rolandas Meškys

  2. Proteomics Center, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio al. 7, 10257, Vilnius, Lithuania

    Algirdas Kaupinis

  3. Centre for Physical Sciences and Technology, Sauletekio al. 3, 10257, Vilnius, Lithuania

    Martynas Skapas

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  1. Laura Kaliniene
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Correspondence to Laura Kaliniene.

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Kaliniene, L., Truncaitė, L., Šimoliūnas, E. et al. Molecular analysis of the low-temperature Escherichia coli phage vB_EcoS_NBD2. Arch Virol 163, 105–114 (2018). https://doi.org/10.1007/s00705-017-3589-5

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  • DOI: https://doi.org/10.1007/s00705-017-3589-5

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