Skip to main content

Advertisement

SpringerLink
Log in

Identification of a serotype-independent linear epitope of foot-and-mouth disease virus

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Foot-and-mouth disease (FMD), caused by foot-and-mouth disease virus (FMDV), is a highly contagious infectious disease that affects domestic and wild cloven-hoofed animals worldwide. VP2 is a structural protein of FMDV. In this study, an FMDV serotype-independent monoclonal antibody (MAb), 10B10, against the viral capsid protein VP2 was generated, and a series of GST fusion proteins expressing a truncated peptide of VP2 was subjected to Western blot analysis using MAb 10B10. Their results indicated that the peptide 8TLLEDRILT16 of VP2 is the minimal requirement of the epitope recognized by MAb 10B10. Importantly, this linear epitope was highly conserved among all seven serotypes of FMDV in a sequence alignment analysis. Subsequent alanine-scanning mutagenesis analysis revealed that the residues Thr8 and Asp12 of the epitope were crucial for MAb-10B10 binding. Furthermore, Western blot analysis also revealed that the MAb 10B10-directed epitope could be recognized by positive sera from FMDV-infected cattle. The discovery that MAb 10B10 recognizes a serotype-independent linear epitope of FMDV suggests potential applications for this MAb in the development of serotype-independent tests for FMDV.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Reference

  1. Bachrach HL (1968) Foot-and-mouth disease. Annu Rev Microbiol 22:201–244

    Article  CAS  PubMed  Google Scholar 

  2. Barteling SJ (2002) Development and performance of inactivated vaccines against foot and mouth disease. Rev Sci Tech 21(3):577–588

    Article  CAS  PubMed  Google Scholar 

  3. Curry S, Fry E, Blakemore W, Abughazaleh R, Jackson T, King A, Lea S, Newman J, Stuart D (1997) Dissecting the roles of VP0 cleavage and RNA packaging in picornavirus capsid stabilization: the structure of empty capsids of foot-and-mouth disease virus. J Virol 71:9743–9752

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Denisova GF, Denisov DA, Yeung J, Loeb MB, Diamond MS, Bramson JL (2008) A novel computer algorithm improves antibody epitope prediction using affinity-selected mimotopes: a case study using monoclonal antibodies against the West Nile virus E protein. Mol immunol 46(1):125–134

    Article  CAS  PubMed  Google Scholar 

  5. Drake JW, Holland JJ (1999) Mutation rates among RNA viruses. Proc Natl Acad Sci USA 96:13910–13913

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Freiberg B, Höhlich B, Haas B, Saalmüller A, Pfaff E, Marquardt O (2001) Type-independent detection of foot-and-mouth disease virus by monoclonal antibodies that bind to amino-terminal residues of capsid protein VP2. J Virol Methods 92(2):199–205

    Article  CAS  PubMed  Google Scholar 

  7. Grubman MJ, Baxt B (2004) Foot-and-mouth disease. Clin Microbiol Rev 17:465–493

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Levy R, Forsyth CM, LaPorte SL, Geren IN, Smith LA, Marks JD (2007) Fine and domain-level epitope mapping of botulinum neurotoxin type A neutralizing antibodies by yeast surface display. J Mol Biol 365(1):196–210

    Article  CAS  PubMed  Google Scholar 

  9. Liang W, Zhou G, Liu W, Yang B, Li C, Wang H, Yang D, Ma W, Yu L (2016) Identification of a conserved linear neutralizing epitope recognized by monoclonal antibody 9A9 against serotype A foot-and-mouth disease virus. Arch Virol 161:2705–2716

    Article  CAS  PubMed  Google Scholar 

  10. Liu W, Yang B, Wang M, Liang W, Wang H, Yang D, Ma W, Zhou G, Yu L (2017) Identification of a conserved conformational epitope in the VP2 protein of foot-and-mouth disease virus. Arch Virol 162:1877–1885

    Article  CAS  PubMed  Google Scholar 

  11. May C, Doody JF, Abdullah R, Balderes P, Xu X, Chen CP, Zhu Z, Shapiro L, Kussie P, Hicklin DJ, Liao F, Bohlen P (2005) Identification of a transiently exposed VE-cadherin epitope that allows for specific targeting of an antibody to the tumor neovasculature. Blood 105(11):4337–4344

    Article  CAS  PubMed  Google Scholar 

  12. Pereira HG (1981) Foot-and-mouth disease. Academic Press Inc., London

    Google Scholar 

  13. Saiz JC, Gonzalez MJ, Borca MV, Sobrino F, Moore DM (1991) Identification of neutralizing antigenic sites on VP1 and VP2 of type A5 foot-and-mouth disease virus, defined by neutralization-resistant variants. J Virol 65(5):2518–2524

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Shen X, Parks RJ, Montefiori DC, Kirchherr JL, Keele BF, Decker JM, Blattner WA, Gao F, Weinhold KJ, Hicks CB, Greenberg ML, Hahn BH, Shaw GM, Haynes BF, Tomaras GD (2009) In vivo gp41 antibodies targeting the 2F5 monoclonal antibody epitope mediate human immunodeficiency virus type 1 neutralization breadth. J Virol 83(8):3617–3625

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Sukupolvi-Petty S, Austin SK, Engle M, Brien JD, Dowd KA, Williams KL, Johnson S, Rico-Hesse R, Harris E, Pierson TC, Fremont DH, Diamond MS (2010) Structure and function analysis of therapeutic monoclonal antibodies against dengue virus type 2. J Virol 84(18):9227–9239

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Yang M, Clavijo A, Suarez-Banmann R, Avalo R (2007) Production and characterization of two serotype independent monoclonal antibodies against foot-and-mouth disease virus. Vet Immunol Immunopathol 115:126–134

    Article  CAS  PubMed  Google Scholar 

  17. Yang M, Goolia M, Xu W, Bittner H, Clavijo A (2013) Development of a quick and simple detection methodology for foot-and-mouth disease virus serotypes O, A and Asia 1 using a generic RapidAssay Device. Virol J 10:125

    Article  PubMed  PubMed Central  Google Scholar 

  18. Yu Y, Wang H, Zhao L, Zhang C, Jiang Z, Li Y (2011) Fine mapping of a foot-and-mouth disease virus epitope recognized by serotype-independent monoclonal antibody 4B2. J Microbiol 49:94–101

    Article  PubMed  Google Scholar 

  19. Zheng H, Lian K, Yang F, Jin Y, Zhu Z, Guo J, Cao W, Liu H, He J, Zhang K, Li D, Liu X (2015) Cross-protective efficacy of engineering serotype A foot-and-mouth disease virus vaccine against the two pandemic strains in swine. Vaccine 33:5772–5778

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by a grant from The National Key Research and Development Program of China (no. 2016YFD0501505).

Author information

Authors and Affiliations

  1. Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 678 Haping Road Xiangfang District, Harbin, 150069, People’s Republic of China

    Baolin Yang, Mingxia Wang, Wenming Liu, Zhiqiang Xu, Haiwei Wang, Decheng Yang, Guohui Zhou & Li Yu

  2. Institute of Veterinary Medicine, Xinjiang Academy of Animal Science, 151 Eastern Kelamayi Street, Ürümqi, 830000, People’s Republic of China

    Wenge Ma

Authors
  1. Baolin Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mingxia Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wenming Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhiqiang Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Haiwei Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Decheng Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Wenge Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Guohui Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Li Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Guohui Zhou.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

Animal welfare and experimental procedures were carried out strictly according to the American Physiological Society’s Guiding Principles for the Care and Use of Animals and were approved by the Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences. The animal ethics committee approval number is SYXK (Hei) 2012–2067. Mice were acclimatized for one week prior to the beginning of the experiment, were bred in clean and spacious animal facilities, and were handled humanely according to the rules described by the Animal Ethics Procedures and Guidelines of the People’s Republic of China and the Animal Ethics Committee of HVRI, Chinese Academy of Agricultural Sciences [SYXK (Hei) 2012–2067]. The mice were euthanized by exsanguination under deep anesthesia (intramuscular injection of chlorpromazine at 2–6 mg/kg) at the end of the experiment.

Additional information

Handling Editor: Tim Skern.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yang, B., Wang, M., Liu, W. et al. Identification of a serotype-independent linear epitope of foot-and-mouth disease virus. Arch Virol 162, 3875–3880 (2017). https://doi.org/10.1007/s00705-017-3544-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-017-3544-5

Keywords

Search

Navigation