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Complete genome sequence of a bovine ephemeral fever virus JT02L strain in mainland China

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Abstract

In this study, we report the complete genome sequence of bovine ephemeral fever virus (BEFV) JT02L, which has been used in our laboratory, in mainland China, for more than a decade. The genome is 14941 nucleotide (nt), comprising a leader sequence of 50 nt, nucleoprotein (N) gene of 1328 nt, phosphoprotein (P) gene of 858 nt, matrix protein (M) gene of 691 nt, glycoprotein (G) gene of 1897 nt, non-structural glycoprotein (GNS) gene of 1785 nt, α1α2 gene of 638 nt, β gene of 460 nt, γ gene of 400 nt, large multi-functional enzyme (L) gene of 6470 nt and a trailer sequence of 73 nt. Individual genes are separated by intergenic regions (IGRs) of 26, 44, 47, 51, 37, 39, 68 and -21 nt respectively. The overall organization is similar to an Australian BEFV isolate BB7721 but demonstrates some distinctive features including longer α3 and β open reading frames, intact termination/polyadenylation (TTP) sequence downstream of the β open reading frame and a longer β-γ IGR integrated with a 38 nt AT-rich fragment. To our knowledge, this is the first report describing the complete genome of a BEFV strain of East Asian lineage, which may facilitate studies on genomic diversity among geographic strains of BEFV in China and the world.

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Acknowledgements

This study was supported by Special Funds for Agro-Scientific Research in the Public Interest (201303035); ASTIP, CAAS, and NBCITS, MOA (CARS-38).

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Authors and Affiliations

  1. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, 730046, Gansu, People’s Republic of China

    Shandian Gao, Junzheng Du, Zhancheng Tian, Qingli Niu, Fuying Zheng, Dexuan Huang, Biao Kang, Jianxun Luo, Guangyuan Liu & Hong Yin

  2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, People’s Republic of China

    Hong Yin

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  1. Shandian Gao
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  2. Junzheng Du
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  3. Zhancheng Tian
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  4. Qingli Niu
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  5. Fuying Zheng
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  6. Dexuan Huang
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  7. Biao Kang
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  8. Jianxun Luo
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  9. Guangyuan Liu
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  10. Hong Yin
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Correspondence to Hong Yin.

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Gao, S., Du, J., Tian, Z. et al. Complete genome sequence of a bovine ephemeral fever virus JT02L strain in mainland China. Arch Virol 162, 3555–3558 (2017). https://doi.org/10.1007/s00705-017-3520-0

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  • DOI: https://doi.org/10.1007/s00705-017-3520-0

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