Abstract
Orf or contagious ecthyma is a neglected and economically important zoonotic disease caused by a dermatotropic parapoxvirus that commonly affects domestic small ruminants. Although orf is globally distributed, there is a paucity of information on the disease in many African countries. Here, a suspected severe outbreak of orf in goats at a farm in Lusaka was investigated. Orf virus (ORFV) infection was confirmed by PCR amplification of viral DNA (RNA polymerase, B2L and virus interferon-resistance genes) in clinical samples. Some detected genes were sequenced and phylogenetically analyzed. This is the first report on molecular characterization of ORFV in goats in Zambia.
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Acknowledgements
The researchers would like to thank the University of Zambia, School of Veterinary Medicine for providing support staff, laboratory and clinical space. This work was supported by the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID) and the Japan Agency for Medical Research and Development (AMED)/Japan International Cooperation Agency (JICA) within the framework of the Science and Technology Research Partnership for Sustainable Development (SATREPS).
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Simulundu, E., Mtine, N., Kapalamula, T.F. et al. Genetic characterization of orf virus associated with an outbreak of severe orf in goats at a farm in Lusaka, Zambia (2015). Arch Virol 162, 2363–2367 (2017). https://doi.org/10.1007/s00705-017-3352-y
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DOI: https://doi.org/10.1007/s00705-017-3352-y