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Dexamethasone treatment decreases replication of viral hemorrhagic septicemia virus in Epithelioma papulosum cyprini cells

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Abstract

The expression of Mx1 in EPC cells after treatment with poly(I:C) or infection with viral hemorrhagic septicemia virus (VHSV) was significantly suppressed by treatment with dexamethasone. However, the titer of VHSV did not increase but instead decreased after dexamethasone treatment. This suggests that dexamethasone not only downregulates type I IFN but also affects certain factors that are necessary for VHSV replication. An important effect of HSP90 on replication of RNA viruses and downregulation of HSP90 by glucocorticoids have been reported. In this study, dexamethasone downregulated HSP90α expression in EPC cells that were stimulated with poly(I:C) or infected with VHSV. Furthermore, cells treated with an HSP90 inhibitor, geldanamycin, showed significantly decreased titers of VHSV, suggesting that HSP90 may be an important host component involved in VHSV replication, and HSP90 inhibition might be one of the causes for the observed reduction in viral titer caused by dexamethasone treatment.

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References

  1. Tordo N, Benmansour A, Calisher C, Dietzgen RG, Fang R-X, Jackson AO, Kurath G, Nadin-Davis S, Tesh RB, Walker PJ (2005) Family Rhabdoviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy: eighth report of the international committee on taxonomy of viruses. Elsevier, San Diego, pp 623–644

    Google Scholar 

  2. Schlotfeldt HJ, Ahne W (1988) Epizootics in brown trout (Salmo trutta fario) caused by VHSV-F1. J Appl Ichthyol 4:147–148

    Article  Google Scholar 

  3. Schlotfeldt HJ, Ahne W, Vestergard-Jorgensen PE, Glende W (1991) Occurrence of viral haemorrhagic septicaemia in turbot (Scophthalmus maximus)—a natural outbreak. EAFP Bull 11:105–107

    Google Scholar 

  4. Mortensen HF, Heuer OE, Lorenzen N, Otte L, Olesen NJ (1999) Isolation of viral haemorrhagic septicaemia virus (VHSV) from wild marine fish species in the Baltic Sea, Kattegat, Skagerrak and the North Sea. Virus Res 63:95–106

    Article  CAS  PubMed  Google Scholar 

  5. Isshiki T, Nagano T, Miyazaki T (2003) Susceptibility of various marine fish species to viral hemorrhagic septicemia virus isolated from Japanese flounder. Fish Pathol 38:113–115

    Article  Google Scholar 

  6. Skall HF, Olesen NJ, Mellergaard S (2005) Viral hemorrhagic septicemia virus in marine fish and its implications for fish farming—a review. J Fish Dis 28:509–529

    Article  CAS  PubMed  Google Scholar 

  7. Einer-Jensen KE, Ahrens P, Forsberg R, Lorenzen N (2004) Evolution of the fish rhabdovirus viral haemorrhagic septicaemia virus. J Virol 85:1167–1179

    Article  CAS  Google Scholar 

  8. Snow M, Bain N, Black J, Taupin V, Cunningham CO, King JA, Skall HF, Raynard RS (2004) Genetic population structure of marine viral haemorrhagic septicaemia virus (VHSV). Dis Aquat Org 61:11–21

    Article  CAS  PubMed  Google Scholar 

  9. Kim WS, Kim SR, Kim D, Kim JO, Park MA, Kitamura SI, Kim HY, Kim DH, Han HJ, Jung SJ, Oh MJ (2009) An outbreak of VHSV (viral hemorrhagic septicemia virus) infection in farmed olive flounder Paralichthys olivaceus in Korea. Aquaculture 296:165–168

    Article  Google Scholar 

  10. Haller O, Kochs G, Weber F (2006) The interferon response circuit: induction and suppression by pathogenic viruses. Virology 344:119–130

    Article  CAS  PubMed  Google Scholar 

  11. Randall RE, Goodbourn S (2008) Interferons and viruses: an interplay between induction, signalling, antiviral responses and virus countermeasures. J Gen Virol 89(Pt 1):1–47

    Article  CAS  PubMed  Google Scholar 

  12. Zou J, Secombes CJ (2011) Teleost fish interferons and their role in immunity. Dev Comp Immunol 35:1376–1387

    Article  CAS  PubMed  Google Scholar 

  13. Faul EJ, Lyles DS, Schnell MJ (2009) Interferon response and viral evasion by members of the family rhabdoviridae. Viruses 1:832–851

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Purcell MK, Laing KJ, Winton JR (2012) Immunity to fish rhabdoviruses. Viruses 4:140–166

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Takami I, Kwon SR, Nishizawa T, Yoshimizu M (2010) Protection of Japanese flounder Paralichthys olivaceus from viral hemorrhagic septicemia (VHS) by Poly(I:C) immunization. Dis Aquat Organ 89:109–115

    Article  CAS  PubMed  Google Scholar 

  16. Flammer JR, Dobrovolna J, Kennedy MA, Chinenov Y, Glass CK, Ivashkiv LB, Rogatsky I (2010) The type I interferon signaling pathway is a target for glucocorticoid inhibition. Mol Cell Biol 30:4564–4574

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Lovy J, Speare DJ, Stryhn H, Wright GM (2008) Effects of dexamethasone on host innate and adaptive immune responses and parasite development in rainbow trout Oncorhynchus mykiss infected with Loma salmonae. Fish Shellfish Immunol 24:649–658

    Article  CAS  PubMed  Google Scholar 

  18. Salas-Leiton E, Coste O, Asensio E, Infante C, Cañavate JP, Manchado M (2012) Dexamethasone modulates expression of genes involved in the innate immune system, growth and stress and increases susceptibility to bacterial disease in Senegalese sole (Solea senegalensis Kaup, 1858). Fish Shellfish Immunol 32:769–778

    Article  CAS  PubMed  Google Scholar 

  19. Hara Y, Shiraishi A, Kobayashi T, Kadota Y, Shirakata Y, Hashimoto K, Ohashi Y (2009) Alteration of TLR3 pathways by glucocorticoids may be responsible for immunosusceptibility of human corneal epithelial cells to viral infections. Mol Vis 15:937–948

    CAS  PubMed  PubMed Central  Google Scholar 

  20. Du T, Zhou G, Roizman B (2012) Induction of apoptosis accelerates reactivation of latent HSV-1 in ganglionic organ cultures and replication in cell cultures. PNAS 109:14616–14621

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Parks WP, Scolnick EM, Kozikowski EH (1974) Dexamethasone stimulation of murine mammary tumor virus expression: a tissue culture source of virus. Science 184:158–160

    Article  CAS  PubMed  Google Scholar 

  22. Indik S, Günzburg WH, Kulich P, Salmons B, Rouault F (2007) Rapid spread of mouse mammary tumor virus in cultured human breast cells. Retrovirology 4:73

    Article  PubMed  PubMed Central  Google Scholar 

  23. Solodushko V, Bitko V, Fouty B (2009) Dexamethasone and mifepristone increase retroviral infectivity through different mechanisms. Am J Physiol Lung Cell Mol Physiol 297:L538–L545

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Lancz G, Whitaker-Dowling P, Marsh YV, Bradley G, Eppstein DA, Hackney JF, Hulick-Swardstrom B (1990) Inhibition of vesicular stomatitis virus replication in dexamethasone-treated L929 cells. Proc Soc Exp Biol Med 193:190–196

    Article  CAS  PubMed  Google Scholar 

  25. Giguere V, Hollenberg SM, Rosenfeld MG, Evans RM (1986) Functional domains of the human glucocorticoid receptor. Cell 46:645–652

    Article  CAS  PubMed  Google Scholar 

  26. Scheinman RI, Cogswell PC, Lofquist AK, Baldwin AS Jr (1995) Role of transcriptional activation of IκBα in mediation of immunosuppression by glucocorticoids. Science 270:283–286

    Article  CAS  PubMed  Google Scholar 

  27. Cadepond F, Schweizer-Groyer G, Segard-Maurel I, Jibard N, Hollenberg SM, Giguere V, Evans RM, Baulieu EE (1991) Heat shock protein 90 as a critical factor in maintaining glucocorticosteroid receptor in a nonfunctional state. J Biol Chem 266:5834–5841

    CAS  PubMed  Google Scholar 

  28. Pratt WB, Morishima Y, Murphy M, Harrell M (2006) Chaperoning of glucocorticoid receptors. Handb Exp Pharmacol 172:111–138

    Article  CAS  Google Scholar 

  29. Connor JH, McKenzie MO, Parks GD, Lyles DS (2007) Antiviral activity and RNA polymerase degradation following Hsp90 inhibition in a range of negative strand viruses. Virology 362:109–119

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Chase G, Deng T, Fodor E, Leung BW, Mayer D, Schwemmle M, Brownlee G (2008) Hsp90 inhibitors reduce influenza virus replication in cell culture. Virology 377:431–439

    Article  CAS  PubMed  Google Scholar 

  31. Smith DR, McCarthy S, Chrovian A, Olinger G, Stossel A, Geisbert TW, Hensley LE, Connor JH (2010) Inhibition of heat-shock protein 90 reduces Ebola virus replication. Antiviral Res 87:187–194

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Geller R, Vignuzzi M, Andino R, Frydman J (2007) Evolutionary constraints on chaperone-mediated folding provide an antiviral approach refractory to development of drug resistance. Genes Dev 21:195–205

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Geller R, Taguwa S, Frydman J (2012) Broad action of Hsp90 as a host chaperone required for viral replication. Biochim Biophys Acta 1823:698–706

    Article  CAS  PubMed  Google Scholar 

  34. Stahl M, Retzlaff M, Nassal M, Beck J (2007) Chaperone activation of the hepadnaviral reverse transcriptase for template RNA binding is established by the Hsp70 and stimulated by the Hsp90 system. Nucleic Acids Res 35:6124–6136

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Momose F, Naito T, Yano K, Sugimoto S, Morikawa Y, Nagata K (2002) Identification of Hsp90 as a stimulatory host factor involved in influenza virus RNA synthesis. J Biol Chem 277:45306–45314

    Article  CAS  PubMed  Google Scholar 

  36. Newton R (2010) Molecular mechanisms of glucocorticoids action: what is important? Thorax 55:603–613

    Article  Google Scholar 

  37. Lindenstrom T, Buchmann K (1998) Dexamethasone treatment increases susceptibility of rainbow trout, Oncorhynchus mykiss (Walbaum), to infections with Gyrodactylus derjavini Mikailov. J Fish Dis 21:29–38

    Article  CAS  Google Scholar 

  38. Nielsen CV, Buchmann K (2003) Increased susceptibility of Atlantic salmon Salmo salar to infections with Gyrodactylus derjavini induced by dexamethasone bath treatment. J Helminthol 77:65–68

    Article  CAS  PubMed  Google Scholar 

  39. Gadan K, Marjara IS, Sundh H, Sundell K, Evensen Ø (2012) Slow release cortisol implants result in impaired innate immune responses and higher infection prevalence following experimental challenge with infectious pancreatic necrosis virus in Atlantic salmon (Salmo salar) parr. Fish Shellfish Immunol 32:637–644

    Article  CAS  PubMed  Google Scholar 

  40. Castro R, Zou J, Secombes CJ, Martin SAM (2011) Cortisol modulates the induction of inflammatory gene expression in a rainbow trout macrophage cell line. Fish Shellfish Immunol 30:215–223

    Article  CAS  PubMed  Google Scholar 

  41. Vijayan MM, Raptis S, Sathiyaa R (2003) Cortisol treatment affects glucocorticoid receptor and glucocorticoid-responsive genes in the liver of rainbow trout. Gen Comp Endocrinol 132:256–263

    Article  CAS  PubMed  Google Scholar 

  42. Sathiyaa R, Campbell T, Vijayan MM (2001) Cortisol modulates HSP90 mRNA expression in primary cultures of trout hepatocytes. Comp Biochem Physiol B Biochem Mol Biol 129:679–685

    Article  CAS  PubMed  Google Scholar 

  43. Yang K, Shi H, Qi R, Sun S, Tang Y, Zhang B, Wang C (2006) Hsp90 regulates activation of interferon regulatory factor 3 and TBK-1 stabilization in Sendai virus-infected cells. Mol Biol Cell 17:1461–1471

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

This research was a part of the project titled ‘Development of Fish Vaccines and Human Resource Training’, funded by the Ministry of Oceans and Fisheries, Korea.

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Authors and Affiliations

  1. Graduate School of Integrated Bioindustry, Sejong University, Seoul, 05006, South Korea

    Min Sun Kim

  2. Incheon Airport Regional Office, National Fishery Products Quality Management Service, Incheon, 22382, South Korea

    Su Jin Lee

  3. Department of Aquatic Life Medicine, Pukyong National University, Busan, 48513, South Korea

    Seung Hyuk Choi & Ki Hong Kim

  4. Department of Aquatic Life and Medical Sciences, Sun Moon University, Asan, 31460, Chungnam, South Korea

    Yue Jai Kang

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Correspondence to Ki Hong Kim.

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All applicable institutional guidelines for the care and use of animals were followed.

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Kim, M.S., Lee, S.J., Choi, S.H. et al. Dexamethasone treatment decreases replication of viral hemorrhagic septicemia virus in Epithelioma papulosum cyprini cells. Arch Virol 162, 1387–1392 (2017). https://doi.org/10.1007/s00705-017-3248-x

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