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Genomic characterization of a bovine viral diarrhea virus subtype 1i in Brazil

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Abstract

Bovine viral diarrhea virus 1 (BVDV-1) belongs to the genus Pestivirus within the family Flaviviridae. Based on the 5’ untranslated region (UTR) sequence, BVDV-1 can be divided into at least 17 subtypes (1a though 1q). BVDV-1i is an uncommon subtype that has been reported in the United Kingdom and Uruguay. Here, we report the complete genome sequence of the first subtype 1i BVDV-1 (strain ACM/BR/2016) isolated from cattle in southern Brazil. The genome is 12,231 nt in length and contains a single ORF that encodes a polyprotein of 3,896 amino acids, flanked by 5’ and 3’UTRs of 325 and 220 nt, respectively. Phylogenetic inferences based on the whole genome, the 5’UTR, and the Npro region showed that strain ACM/BR/2016 is closely related to previously characterized BVDV-1i members. Its 5’UTR shares the highest nucleotide identity (90.5%) with BVDV-1i strains from United Kingdom, and its Npro is most closely related to that of a Uruguayan strain (90.6%). To the best of our knowledge, this is the first BVDV-1i strain from which the whole genome has been completely sequenced and characterized. The complete genome of a BVDV-1i will help future studies on pestivirus evolution and heterogeneity.

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Acknowledgements

The authors thank Setor de Patologia Veterinária (Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul) for providing isolates, and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and PROPESQ/UFRGS for supporting this study.

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Authors and Affiliations

  1. Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves 9090, Porto Alegre, RS, CEP 91540-000, Brazil

    Ana Cristina S. Mósena, Matheus N. Weber, Samuel P. Cibulski, Simone Silveira, Mariana S. Silva & Cláudio W. Canal

  2. Laboratório de Biologia Molecular, Instituto de Pesquisas Veterinárias Desidério Finamor, Fundação Estadual de Pesquisa Agropecuária, Eldorado do Sul, RS, Brazil

    Ana Cristina S. Mósena & Fabiana Q. Mayer

Authors
  1. Ana Cristina S. Mósena
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  2. Matheus N. Weber
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  3. Samuel P. Cibulski
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  4. Simone Silveira
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  5. Mariana S. Silva
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  6. Fabiana Q. Mayer
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  7. Cláudio W. Canal
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Correspondence to Cláudio W. Canal.

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The authors whose names are listed below certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria, educational grants, participation in speakers’ bureaus, membership employment, consultancies, stock ownership, or other equity interest, and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript. We have no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Mósena, A.C.S., Weber, M.N., Cibulski, S.P. et al. Genomic characterization of a bovine viral diarrhea virus subtype 1i in Brazil. Arch Virol 162, 1119–1123 (2017). https://doi.org/10.1007/s00705-016-3199-7

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  • DOI: https://doi.org/10.1007/s00705-016-3199-7

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