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Merkel cell polyomavirus and human papillomavirus infections in cervical disease in Iranian women

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Abstract

Human papillomavirus (HPV) infection is a necessary cause of cervical neoplasia. Concomitant infection with other infectious agents has been demonstrated to be a cofactor for HPV-related cervical carcinogenesis. The present investigation aimed to determine the prevalence of HPV and Merkel cell polyomavirus (MCPyV) infections and to evaluate the role of MCPyV as a co-factor for HPV-related cervical carcinogenesis in Iranian women. From 2011 to 2013, a total of 112 cervical samples were examined. Forty-five samples (40.2 %) were positive for HPV. MCPyV was found in 37 samples (33 %). Both HPV and MCPyV were present in 14 samples (12.5 %). MCPyV was seen in 30 % of squamous cell carcinomas, 37.5 % of adenocarcinomas, and 16.7 % of undifferentiated carcinomas. The MCPyV large T antigen (LT-Ag) DNA load was determined as the viral copy number per cell. The median MCPyV LT-Ag copy number in positive women was 0.049 × 10−3 per cell (range 0.0006 × 10−3-4.558 × 10−3 copies per cell). In comparison with other types of cervical cancer, the MCPyV LT-Ag load was higher in adenocarcinomas (0.1024 × 10−3 copies per cell). A logistic regression model adjusted to HPV positivity and age revealed no statistically significant association between MCPyV infection and cervical cancer (OR, 1.12; 95 % CI, 0.07-16.83). More studies should be conducted to clarify the role of MCPyV in cervical carcinogenesis.

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Acknowledgments

The authors would like to thank the directors and staff of the Cancer Institute of Imam Hospital Complex affiliated to Tehran University of Medical Sciences for their assistance in sample collection. This work was supported through a grant from the Tehran University of Medical Sciences (Project code: 92-01-30-21080).

Conflict of interest

None to declare.

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Authors and Affiliations

  1. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Mostafa Salehi-Vaziri & Farzin Sadeghi

  2. Department of Immunology and Microbiology, School of Medicine, Babol University of Medical Sciences, Babol, Iran

    Farzin Sadeghi

  3. Department of Epidemiology, School of Health, Arak University of Medical Sciences, Arak, Iran

    Amir Alamsi-Hashiani

  4. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Amir Alamsi-Hashiani

  5. Department of Pathology, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran

    Hayedeh Haeri

  6. Department of Virology, School of Medicine, Iran University of Medical Sciences, Junction of Shahid Hemmat and Shahid Chamran Expressways, P.O. Box 1449614535, Tehran, Iran

    Seyed Hamidreza Monavari & Hossein Keyvani

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  1. Mostafa Salehi-Vaziri
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  2. Farzin Sadeghi
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  6. Hossein Keyvani
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Correspondence to Hossein Keyvani.

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Salehi-Vaziri, M., Sadeghi, F., Alamsi-Hashiani, A. et al. Merkel cell polyomavirus and human papillomavirus infections in cervical disease in Iranian women. Arch Virol 160, 1181–1187 (2015). https://doi.org/10.1007/s00705-015-2368-4

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  • DOI: https://doi.org/10.1007/s00705-015-2368-4

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