Abstract
Hippeastrum chlorotic ringspot virus (HCRV) is a novel tospovirus that was identified in Yunnan Province, China, in 2013. We have sequenced the HCRV L gene, which is 8909 nt long and encodes the RNA-dependent RNA polymerase (2873 amino acids, 330.8 kDa). The HCRV L protein shared highest similarity (89.4 %) with that of tomato yellow ring virus. The L protein contains a negative-sense RNA virus RNA-directed RNA polymerase motif and an endonuclease domain at the N-terminus. Combined with our previous reports of the S and M RNAs, the genome sequence of HCRV is now completed.
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Acknowledgments
This work was supported by the National Science Foundation of China (No. 31260451 & 30960224), a Project on Social Development by the Department of Science and Technology of Yunnan Province, National Undergraduate Training Programs for Innovation and Entrepreneurship (201210676006), and Yunnan Undergraduate Training Programs for Innovation and Entrepreneurship (DC0001).
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The authors have no conflicts of interest to declare.
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Y. Xu and S. Lou contributed equally to this work.
The nucleotide sequence data reported in this manuscript have been deposited at the EMBL under accession No. HG763861.
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Xu, Y., Lou, Sg., Li, Qf. et al. Molecular characterization of the hippeastrum chlorotic ringspot virus L segment and its protein. Arch Virol 159, 2805–2807 (2014). https://doi.org/10.1007/s00705-014-2100-9
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DOI: https://doi.org/10.1007/s00705-014-2100-9