Abstract
A novel virulent phage named JL1 against Escherichia coli O157:H7 was isolated from raw sewage. It was found that JL1 has an icosahedral head and a long flexible non-contractile tail. The complete genome of JL1 is composed of a linear double-stranded DNA of 43,457 base pairs in length, with 54.77 % G+C content and 60 putative open reading frames. Morphology and bioinformatics analysis revealed that phage JL1 is a member of the family Siphoviridae of the order Caudovirales. It is different from previously reported phages of E. coli O157:H7 but is homologous to Sodalis phage SO-1, Shigella phage EP23, Escherichia phage HK578 and Escherichia phage SSL-2009a.
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Acknowledgment
This work was supported by the Technical Center of Jilin Entry-Exit Inspection and Quarantine Bureau (JLCIQ) of People’s Republic China, and by grants from the General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China (Project Code: No. 2012IK163).
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Pan, F., Wu, H., Liu, J. et al. Complete genome sequence of Escherichia coli O157:H7 lytic phage JL1. Arch Virol 158, 2429–2432 (2013). https://doi.org/10.1007/s00705-013-1727-2
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DOI: https://doi.org/10.1007/s00705-013-1727-2