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Comparison of complete polyprotein sequences of two isolates of salmon alphavirus (SAV) type I and their behaviour in a salmonid cell line

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Abstract

Salmon pancreas disease virus is an alphavirus (family Togaviridae) affecting mainly Atlantic salmon (Salmo salar L.). Both polyprotein sequences of the Scottish isolate (SAV4640) were determined and compared with those of Irish isolate SAVF93-125. High amino acid sequence similarity (99.4 %) was found. Six amino acid deletions were found in the E2 gene of SAV4640. SAVF93-125 demonstrated a high viral load in culture despite high Mx expression. Approximately 50 % of cells infected with SAVF93-125 exhibited a cytopathic effect by day 8. SAV4640 successfully entered the cells, inducing 10,500-fold higher Mx expression at day 2 compared to SAVF93-25; however, no replication was observed based on results of the nsP1 qRT-PCR.

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Acknowledgments

ZL was supported by the British Council (IAESTE). JB was supported by the Canary Islands Government (PI042002/153).

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Authors and Affiliations

  1. Marine Scotland Science, 375 Victoria Road, Aberdeen, AB11 9DB, Scotland, UK

    Iveta Matejusova, Katherine Lester, Ziduo Li, Jimena Bravo, Fiona Bland & Bertrand Collet

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  1. Iveta Matejusova
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  2. Katherine Lester
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  3. Ziduo Li
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  4. Jimena Bravo
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  5. Fiona Bland
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  6. Bertrand Collet
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Correspondence to Iveta Matejusova.

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Matejusova, I., Lester, K., Li, Z. et al. Comparison of complete polyprotein sequences of two isolates of salmon alphavirus (SAV) type I and their behaviour in a salmonid cell line. Arch Virol 158, 2143–2146 (2013). https://doi.org/10.1007/s00705-013-1689-4

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  • DOI: https://doi.org/10.1007/s00705-013-1689-4

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