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Pathogen-derived resistance using a viral nucleocapsid gene confers only partial non-durable protection in peanut against peanut bud necrosis virus

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Abstract

Genetic engineering of peanut (Arachis hypogaea L.) using the gene encoding for the nucleocapsid protein (N gene) of peanut bud necrosis virus (PBNV; genus Tospovirus, family Bunyaviridae) was used to impart resistance to bud necrosis disease in peanut (PBND), a disease for which no durable resistance is available in the existing germplasm. Over 200 transgenic lines of peanut var. JL 24 were developed for which integration and expression of the transgenes was confirmed by PCR, Southern hybridization, RT-PCR and western blot analysis. The T1 and T2 generation transgenic plants were assayed through virus challenge in the greenhouse by using mechanical sap inoculation at 1:100 and 1:50 dilutions of PBNV, and they showed varying levels of disease incidence and intensity. Greenhouse and field evaluation with T2 generation plants indicated somewhat superior performance of the three transgenic events that showed considerable reduction in disease incidence. However, only one of these events showed over 75 % reduction in disease incidence when compared to the untransformed control, indicating partial and non-durable resistance to PBND using the viral N-gene.

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Acknowledgments

We thank T. Swami Krishna, D. Pandary, Md. Yousuf, and C. Lakshminarayana for excellent technical assistance during this study. SCR would like to thank the Jawaharlal Nehru Technological University, Hyderabad, for providing an opportunity to register for the Ph.D. program.

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Author notes

  1. S. Chander Rao

    Present address: Directorate of Oilseeds Research (DOR), Rajendranagar, Hyderabad, 500 030, India

  2. P. Lava Kumar

    Present address: International Institute of Tropical Agriculture (IITA), Oyo Road, PMB 5320, Ibadan, Nigeria

Authors and Affiliations

  1. Genetic Transformation Laboratory, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, 502 324, India

    P. Bhatnagar-Mathur, A. Sudarshan Reddy & Kiran K. Sharma

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  1. S. Chander Rao
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  2. P. Bhatnagar-Mathur
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  3. P. Lava Kumar
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  4. A. Sudarshan Reddy
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  5. Kiran K. Sharma
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Correspondence to Kiran K. Sharma.

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Rao, S.C., Bhatnagar-Mathur, P., Kumar, P.L. et al. Pathogen-derived resistance using a viral nucleocapsid gene confers only partial non-durable protection in peanut against peanut bud necrosis virus. Arch Virol 158, 133–143 (2013). https://doi.org/10.1007/s00705-012-1483-8

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