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Detection, prevalence and analysis of emerging porcine parvovirus infections

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Abstract

A number of newly identified porcine parvoviruses had been described during the last decade, but the presence and prevalence of these viruses are unknown in Hungary and only partly known for Europe. The present study was conducted to detect and measure the prevalence of these viruses, namely porcine parvovirus (PPV) 2, PPV3, PPV4, porcine bocavirus (PBoV) 1, PBoV2, PBo-likeV and the 6V and 7V parvoviruses. The prevalence of PPV1 and porcine circovirus type 2 (PCV2) was also investigated. Faecal samples, blood serum samples, organ tissues, foetuses and semen were collected from different swine herds in Hungary and tested by polymerase chain reaction methods specific for the different viruses. The results indicated that all of the examined parvoviruses were present in Hungary, hence in Europe. The prevalence was 18.1% for PCV2, 0.5 % for PPV1, 6.4% for PPV2, 9.7% for PPV3, 6.4% for PPV4, 1.5% for PBo-likeV, 4.8% for PBoV1 and PBoV2 and 1.8% for 6V and 7V. Based on the analysis of partial PPV4 and PBo-likeV sequences, these viruses showed a high degree of sequence conservation, whereas PPV3 and the majority of PPV2, PBoV1, PBoV2, 6V and 7V sequences showed higher variability. Possible sites of recombination were also identified between PBoV1 and PBoV2 genomes.

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Acknowledgments

This paper was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Science, grant NKB15945 from the Szent István University and OTKA research grant PD100405.

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Authors and Affiliations

  1. Faculty of Veterinary Science, Department of Microbiology and Infectious Diseases, Szent István University, Hungária krt. 23–25, 1143, Budapest, Hungary

    Attila Cságola, Márta Lőrincz, Kata Tombácz & Tamás Tuboly

  2. Faculty of Veterinary Science, Large Animals Clinic, Szent István University, Dóra major, 2225, Üllő, Hungary

    Imre Biksi

  3. Department of Infectious Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Manastur 3-5, 400372, Cluj-Napoca, Romania

    Dániel Cadar

Authors
  1. Attila Cságola
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  2. Márta Lőrincz
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  3. Dániel Cadar
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  4. Kata Tombácz
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  5. Imre Biksi
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  6. Tamás Tuboly
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Corresponding author

Correspondence to Tamás Tuboly.

Additional information

Nucleotide sequence data reported here are available in the GenBank database under the following accession numbers: JN400849 (PBo-likeV), JN400850-N400861 (PPV2), JN400862-JN400868 (PPV3), JN400869 (PPV4), JN400870-JN400875 (PBoV), JN400876-JN400879 (6V7V).

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Cságola, A., Lőrincz, M., Cadar, D. et al. Detection, prevalence and analysis of emerging porcine parvovirus infections. Arch Virol 157, 1003–1010 (2012). https://doi.org/10.1007/s00705-012-1257-3

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  • DOI: https://doi.org/10.1007/s00705-012-1257-3

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