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Genetic diversity in the 3′-terminal region of papaya ringspot virus (PRSV-W) isolates from watermelon in Oklahoma

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Abstract

The 3′-terminal region (1191 nt) containing part of the NIb gene, complete coat protein (CP) and poly-A tail of 64 papaya ringspot virus (PRSV-W) isolates collected during 2008-2009 from watermelon in commercial fields of four different counties of Oklahoma were cloned and sequenced. Nucleotide and amino acid sequence identities ranged from 95.2-100% and 97.1-100%, respectively, among the Oklahoman PRSV-W isolates. Phylogenetic analysis showed that PRSW-W isolates clustered according to the locations where they were collected within Oklahoma, and each cluster contained two subgroups. All subgroups of Oklahoman PRSV-W isolates were on separate branches when compared to 35 known isolates originating from other parts of the world, including the one reported previously from the USA. This study helps in our understanding about the genetic diversity of PRSV-W isolates infecting cucurbits in Oklahoma.

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References

  1. Ali A, Mohammad O, Khattab A (2011) Distribution of viruses infecting cucurbit crops and isolation of potential new virus-like sequences from weeds in Oklahoma. Plant Dis. doi:10.1094/PDIS-05-11-0419

  2. Atreya CD, Raccah B, Pirone TP (1990) A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus. Virology 178:161–165

    Article  PubMed  CAS  Google Scholar 

  3. Attasart P, Charoensilp G, Kertbundit S, Panyim S, Juricek M (2002) Nucleotide sequence of a Thai isolate of Papaya ringspot virus type W. Acta Virol 46:241–246

    Google Scholar 

  4. Bateson MF, Henderson J, Chaleeprom W, Gibbs AG, Dale JL (1994) Papaya ringspotPotyvirus: isolates variability and the origin of PRSV type (Australia). J Gen Virol 75:3547–3553

    Article  PubMed  CAS  Google Scholar 

  5. Bateson MF, Lines RE, Revill P, Chaleeprom W, Ha CV, Gibbs AJ, Dale JL (2002) On the evolution and molecular epidemiology of the potyvirus Papaya ringspot virus. J Gen Virol 83:2575–2585

    PubMed  CAS  Google Scholar 

  6. Bousalem M, Douzery EJE, Fargette D (2000) High genetic diversity, distant phylogenetic relationship and interspecies recombination events among natural populations of Yam mosaic virus: a contribution to understanding potyvirus evolution. J Gen Virol 81:243–255

    PubMed  CAS  Google Scholar 

  7. Chin M, Rojas Y, Moret J, Fermin G, Tennant P, Gonsalves D (2007) Varying genetic diversity of Papaya ringspot virus isolates from two time-separated outbreaks in Jamaica and Venezuela. Arch Virol 152:2101–2106

    Article  PubMed  CAS  Google Scholar 

  8. Davis MJ, Ying Z (1999) Genetic diversity of the Papaya ringspot virus in Florida. Proc Fla State Hortic Soc 112:194–196

    Google Scholar 

  9. Fernandez-Rodriguez T, Rubio L, Carballo O, Marys E (2008) Genetic variation of Papaya ringspot virus in Venezuela. Arch Virol 153:343–349

    Article  PubMed  CAS  Google Scholar 

  10. Frenkel MJ, Ward CW, Shukla DD (1989) The use 3 non-coding nucleotide sequences in the taxonomy of potyviruses: Application to Watermelon mosaic virus 2 and Soybean mosaic virus. J Gen Virol 68:297–304

    Google Scholar 

  11. Gal-On A, Antignus Y, Rosner A, Raccah B (1992) A Zucchini yellow mosaic virus coat protein gene mutation restores aphid transmissibility but has no effect on multiplication. J Gen Virol 73:2183–2187

    Article  PubMed  CAS  Google Scholar 

  12. Gonsalves D, Ishii M (1980) Purification and serology of Papaya ringspot virus. Phytopathology 70:1028–1032

    Article  CAS  Google Scholar 

  13. Gonsalves D (1998) Control of Papaya ringspot virus in papaya: a case study. Ann Rev Phytopathol 36:415–437

    Article  CAS  Google Scholar 

  14. Hema MV, Prasad DT (2004) Comparison of the coat protein of a south Indian strain of PRSV with other strains from different geographical locations. J Plant Pathol 86:35–42

    CAS  Google Scholar 

  15. Huttinga A (1975) Properties of viruses of the potyvirus group. 3. A comparison of buoyant density, S. value, partical morphology and molecular weight of the coat protein subunit of 10 viruses and virus isolates. Neth J Plant Pathol 81:58–63

    Article  CAS  Google Scholar 

  16. Inoue-Nagata AK, Franco CDM, Martin DP, Rezende JAM, Ferreira GB, Dutra LS, Nagata T (2007) Genome analysis of a sever and a mild isolate of Papaya ringspot virus-type W found in Brazil. Virus Genes 35:119–127

    Article  PubMed  CAS  Google Scholar 

  17. Jadao AS, Buriola JE, Rezende JAM (2010) First report of Papaya ringspot virus type W and Zucchini yellow mosaic virus infecting Trichosanthescucumerina in Brazil. Plant Dis 94:789

  18. Jain RK, Pappu HR, Pappu SS, Varma AV, Ram RD (1998) Molecular characterization of Papaya ringspot virus isolates from India. Ann Appl Biol 32:413–425

    Article  Google Scholar 

  19. Jain RK, Nasiruddin KM, Sharma J, Pant RP, Varma A (2004) First report of occurrence of Papaya ringspot virus in Bangladesh. Plant Dis 88:221

    Article  Google Scholar 

  20. Jain RK, Sharma J, Sivakumar AS, Sharma PK, Byadgi AS, Verma AK, Varma A (2004) Variability in the coat protein gene of Papaya ringspot virus isolates from multiple locations in India. Arch Virol 149:2435–2442

    Article  PubMed  CAS  Google Scholar 

  21. Jensen DD (1949) Papaya ringspot virus and its insect vector relationship. Phytopathology 39:212–220

    Google Scholar 

  22. Lima RCA, Souza JR MT, Pio-Riberro G, Lima JA (2002) Sequences of the coat protein gene from Brazilian isolates of Papaya ringspot virus. Fitopatol Bras 27:174–180

    Article  CAS  Google Scholar 

  23. Lu YW, Shen WT, Zhou P, Tang QJ, Niu YM, Peng M, Xiong Z (2008) Complete genomic sequence of Papaya ringspot virus isolate from Hainan Island, China. Arch Virol 153:991–993

    Article  PubMed  CAS  Google Scholar 

  24. Mangrauthia SK, Parameswari B, Jain RK, Praveau S (2008) Role of genetic recombination in the molecular architecture of Papaya ringspot virus. Biochem Genet 46:835–846

    Google Scholar 

  25. Noa-Carrazana JC, Gonzalez D, Ruiz-Castro BS, Pinero D, Silva-Rosales L (2006) Distribution of Papaya ringspot virus and Papaya mosaic virus in papaya plants (Carcia papaya) in Mexico. Plant Dis 90:1004–1011

    Article  Google Scholar 

  26. Noa-Carrazana JC, Gonzalez D, Silva-Rosales L (2007) Molecular characterization of a sever isolate of Papaya ringspot virus in Mexico and its relation with other isolates. Virus genes 35:109–117

    Article  PubMed  CAS  Google Scholar 

  27. Olarte Castillo XA, Fermin G, Tabima J, Rojas Y, Tennant PF, Fuchs M, Sierra R, Bernal AJ, Restrepo S (2011) Phylogeography and molecular epidemiology of Papaya ringspot virus. Virus Res 159:132–140

    Google Scholar 

  28. Pinel AN, Guessan P, Bousalem M, Fargette D (2000) Molecular variability of geographically distinct isolates of Rice yellow mottle virus in Africa. Arch Virol 145:1621–1638

    Article  PubMed  CAS  Google Scholar 

  29. Purcifull DE, Hiebert E (1979) Serological distinction of Watermelon mosaic virus isolates. Phytopathology 69:112–116

    Article  Google Scholar 

  30. Purcifull DE, Edwardson JR, Hiebert E, Gonsalves D (1984) Papaya ringspot virus. CMI/AAB Description of plant viruses no 292

  31. Quemada H, Hostis LB, Gonsalves D, Reardon IM, Hendrickson R, Hiebert EL, Sieu LC, Slightom JL (1990) The nucleotide sequences of the 3-terminal region of Papaya ringspot virus strain W and P. J Gen Virol 71:203–210

    Article  PubMed  CAS  Google Scholar 

  32. Riechmann JL, lain S, Garcia JA (1992) Highlights and prospects of potyvirus molecular biology. J Gen Virol 73:1–16

    Article  PubMed  CAS  Google Scholar 

  33. Shukla DD, Ward CW (1989) Structure of potyvirus coat proteins and its application in the taxonomy of the potyvirus group. Adv Virus Res 36:273–314

    Google Scholar 

  34. Silva-Rosales L, Ruiz-Castro S, Teliz-Ortiz D, Noa-Carrazena JC (2000) Coat protein sequence comparison of three Mexican isolates of Papaya ringspot virus with other geographical isolates reveal a close relationship to American and Australian isolates. Arch Virol 145:835–843

    Article  PubMed  CAS  Google Scholar 

  35. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Google Scholar 

  36. Tomlinson JA (1987) Epidemiology and control of viruses’ diseases of vegetables. Ann Appl Biol 110:661–681

    Article  Google Scholar 

  37. Tripathi S, Suzuki JY, Ferreira SA, Gonsalves D (2008) Papaya ringspot virus-P: characteristics, pathogenicity, sequence variability and control. Mol Plant Pathol 9:269–280

    Google Scholar 

  38. Wang C, Bau H, Yeh S (1994) Comparison of the nuclear inclusion b protein and coat protein genes of five Papaya ringspot virus strains distinct in geographic origin and pathogenicity. Phytopathology 84:1205–1210

    Article  CAS  Google Scholar 

  39. Wang CH, Yeh SD (1997) Divergence and conservative of the genomic RNAs of Taiwan and Hawaii strains of Papaya ringspot virus. Arch Virol 142:271–285

    Article  PubMed  CAS  Google Scholar 

  40. Wei J, Liu D, Li X, Zhou P (2007) Variation in the coat protein gene of Papaya ringspot virus isolates from multiple locations of China. J Integr Plant Biol 49:1062–1069

    Article  CAS  Google Scholar 

  41. Yeh SD, Gonsalves D (1984) Evaluation of induced mutants of Papaya ring spot virus for control by cross protection. Phytopathology 74:1086–1091

    Article  Google Scholar 

  42. Yeh SD, Jan FJ, Chiange CH, Doonge TJ, Chen MC, Chung PH, Bau HJ (1992) Complete nucleotide sequence and genetic organization of Papaya ring spot virus RNA. J Gen Virol 73:2531–2541

    Google Scholar 

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Acknowledgments

We thank Peggy Hill for careful review of this paper. We are also thankful to Lynn Brandenberger at Oklahoma State University (OSU) Stillwater, Sue Gray, Tulsa County OSU Extension, and Benny Bruton at USDA-ARS, Lane, Oklahoma, for their consistent support during the collection of samples. This work was supported partly by Oklahoma NSF EPSCOR award number EPS-0447262 and Office of Research and Sponsored Program, The University of Tulsa, Grant number 2021211474.

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  1. Department of Biological Science, The University of Tulsa, 800 S. Tucker Dr, Tulsa, OK, 74104, USA

    Osama A. Abdalla & Akhtar Ali

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  1. Osama A. Abdalla
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  2. Akhtar Ali
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Correspondence to Akhtar Ali.

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Abdalla, O.A., Ali, A. Genetic diversity in the 3′-terminal region of papaya ringspot virus (PRSV-W) isolates from watermelon in Oklahoma. Arch Virol 157, 405–412 (2012). https://doi.org/10.1007/s00705-011-1184-8

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