Abstract
Human urine has been poorly investigated with regard to infection with human immunodeficiency virus (HIV). Here, we have studied the anti-infective functional properties of human urine against HIV. The effect of fresh urine pools on CCR5- and CXCR4-tropic HIV-1 was evaluated by using four in vitro mucosal models: reduction of infectivity of urine-treated HIV-1 particles, HIV-1 attachment to immature monocyte-derived dendritic cells (iMDDC), transfer of HIV-1 particles from iMDDC to autologous CD4 T cells, and HIV-1 transcytosis through epithelial cells. Human urine partially disrupted both CCR5- and CXCR4-tropic HIV-1 particles, moderately decreased the adsorption of HIV-1 on dendritic cells, and partially decreased the transfer of HIV-1 particles from dendritic cells to autologous T cells. These findings demonstrate partial inactivation of HIV infectivity and suggest that voiding urine after coitus could play a potential role in reducing the risk of HIV infection by both mechanically flushing out and neutralizing the infectivity of HIV-1 particles present in the genital tract.
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Grant support was received from the Agence Nationale de Recherches sur le SIDA et les Hépatites Virales (ANRS) and Institut National de la Santé et de la Recherche Médicale (INSERM), France.
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Auvert, B., Jenabian, MA., Saidi, H. et al. Partial inactivation of CCR5- and CXCR4- tropic HIV-1 by human urine. Arch Virol 156, 2181–2186 (2011). https://doi.org/10.1007/s00705-011-1114-9
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DOI: https://doi.org/10.1007/s00705-011-1114-9