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The dengue virus nonstructural protein 1 (NS1) increases NF-κB transcriptional activity in HepG2 cells

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Abstract

Dengue virus nonstructural protein 1 (NS1) is a glycoprotein involved in viral RNA replication. NS1 associates with host cell proteins and can be found in lipid raft domains on the host cell surface, suggesting an involvement in signal transduction events. In this work, we observed that NS1 expression in HepG2 cells increases nuclear translocation of NF-κB p65 protein, which was paralleled by DNA-protein complex formation. Luciferase assays showed an increase in NF-κB transcriptional activities in NS1-expressing cells when compared to parental cells. NS1 may enhance NF-κB function in host cells and contribute to the pathogenesis of dengue.

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Acknowledgments

The authors are grateful to Angela S. Lopes, Ilda M.V. Gama, João R. dos Santos, Helena R. C. Araújo, Fernanda Gambogi, Juliana P.V. Silva and Camila R. C. Nogueira for their secretarial/technical assistance. This work was supported by grants from FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and INCT Dengue (Institute for Science and Technology in Dengue).

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Authors and Affiliations

  1. Grupo de Transdução de Sinal, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil

    Breno M. Silva, Flávia G. G. Leite & Cláudio A. Bonjardim

  2. Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil

    Alessandra C. Gomes-Ruiz, Flávio G. da Fonseca, Paulo C. P. Ferreira & Erna G. Kroon

  3. Imunofarmacologia, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

    Lirlândia P. Sousa & Mauro M. Teixeira

  4. Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

    Lirlândia P. Sousa

  5. Laboratório de Entomologia Médica, Centro de Pesquisas René Rachou, FIOCRUZ, Av. Augusto de Lima, Belo Horizonte, Minas Gerais, 1715 30.190-002, Brazil

    Breno M. Silva & Paulo F. P. Pimenta

Authors
  1. Breno M. Silva
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  2. Lirlândia P. Sousa
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  3. Alessandra C. Gomes-Ruiz
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  4. Flávia G. G. Leite
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  5. Mauro M. Teixeira
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  6. Flávio G. da Fonseca
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  7. Paulo F. P. Pimenta
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  8. Paulo C. P. Ferreira
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  9. Erna G. Kroon
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  10. Cláudio A. Bonjardim
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Corresponding author

Correspondence to Breno M. Silva.

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Silva, B.M., Sousa, L.P., Gomes-Ruiz, A.C. et al. The dengue virus nonstructural protein 1 (NS1) increases NF-κB transcriptional activity in HepG2 cells. Arch Virol 156, 1275–1279 (2011). https://doi.org/10.1007/s00705-011-0969-0

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  • DOI: https://doi.org/10.1007/s00705-011-0969-0

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